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[177671_at]

Description K03D3.2 /REP_DB=WormBase Gene ID /WP=CE16208 /TR=O45640 /GB=CAB05239.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 71      4grp_total > 03      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 23.8
(-2.2)
P 		3.2
(-3)
A 		8.4
(-2.8)
A 		154.4
(3)
P 		151
B 17.7
(-2.5)
A 		13.5
(-2.7)
A 		8.1
(-3)
A 		120.3
(3)
P 		112
C 29.6
(-2)
A 		21.2
(-2.3)
A 		3.2
(-3)
A 		160.1
(3)
P 		157
avr 23.7
(-2.3) 		12.6
(-2.7) 		6.6
(-3) 		144.9
(3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 39
(-2.8)
A 		49.3
(-2)
P 		65.2
(-0.8)
P 		115.7
(3)
P 		79.2
(0.2)
P 		36.5
(-3)
A 		79
B 26.4
(-2.7)
P 		25.2
(-2.8)
P 		77.4
(0.3)
P 		124.4
(3)
P 		100.5
(1.6)
P 		21.3
(-3)
A 		103
C 32.7
(-2.4)
A 		32.9
(-2.4)
M 		85.4
(1.6)
P 		103.5
(3)
P 		77
(1)
P 		25
(-3)
A 		79
avr 32.7
(-2.6) 		35.8
(-2.4) 		76
(0.3) 		114.5
(3) 		85.6
(1) 		27.6
(-3) 		87
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 8.7
(-0.8)
P 		0.7
(-3)
A 		2.2
(-2.6)
A 		22.6
(3)
P 		22
B 5.7
(-2)
A 		3.3
(-2.6)
A 		1.9
(-3)
A 		25.6
(3)
P 		24
C 9.8
(-1)
A 		6.1
(-1.8)
A 		1.1
(-3)
A 		27
(3)
P 		26
avr 8.1
(-1.4) 		3.4
(-2.6) 		1.7
(-3) 		25.1
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 7.2
(-3)
A 		17.5
(-0.2)
P 		14.2
(-1.1)
P 		29.4
(3)
P 		23.9
(1.5)
P 		8.9
(-2.5)
A 		22
B 9.2
(-2.8)
P 		8.4
(-2.9)
P 		18.8
(-1)
P 		32.9
(1.6)
P 		40.8
(3)
P 		7.9
(-3)
A 		33
C 12.1
(-1.7)
A 		10
(-2.4)
M 		21.7
(1.8)
P 		20.8
(1.5)
P 		24.9
(3)
P 		8.4
(-3)
A 		17
avr 9.5
(-2.7) 		12
(-2) 		18.2
(-0.3) 		27.7
(2.4) 		29.9
(3) 		8.4
(-3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177671_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8669
Sequence Type Exemplar sequence
Representative Public ID K03D3.2 NCBI
Target Description K03D3.2 /REP_DB=WormBase Gene ID /WP=CE16208 /TR=O45640 /GB=CAB05239.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070543(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070543 NCBI Caenorhabditis elegans K03D3.2 (K03D3.2) mRNA, complete cds. 11/11 None
SNAP00000040352 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16328096:16328575:-1 11/11 None
GENEFINDER00000040360 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16328096:16328575:-1 11/11 None
K03D3.2 ENSEMBL cdna:known chromosome:CEL140:IV:16328096:16328575:-1 gene:K03D3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16328101-16328581(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K03D3.2
Entrez Gene 186933 Entrez gene
SwissProt O45640 EMBL-EBI
WormBase CE16208 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05239 Hypothetical protein K03D3.2 [Caenorhabditis elegans] ref|NP_502944.1| K03D3.2 [Caenorhabditis elegans] 8.0E-69
blast AAC68965 Hypothetical protein F58E2.2 [Caenorhabditis elegans] ref|NP_500354.1| F58E2.2 [Caenorhabditis elegans] 2.0E-52
Sequence
Target Sequence 
>C. ELEGANS:177671_AT
gtttttccttattctatttctagcaactatttcttattcgtcccaattaattgccgaatc
tgaattggtggatttcgttgtagctggatcgaactatgactataggacagatttcaccgg
gggagagaagacaattggaataaaaagattgttgaatggcctggaagatgacgatgataa
atggcaattatattatttttgtgcaaaagccaaaggcgcgaataagaagaactgtggatc
gtgggttgatggaaagggaaacaaaatcaaaggagaagtcacccttaaagcaactctcaa
gggcaagaatgtcgtcctgggcccagtttttgttagagatgcaggacgttatgctagcat
tttcgaggattcgaaacagcaaacttcggttgtccaggttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTTTCCTTATTCTATTTCTAGCA 255 449 15 Antisense
TAGCAACTATTTCTTATTCGTCCCA 558 651 35 Antisense
TCGTCCCAATTAATTGCCGAATCTG 143 603 52 Antisense
GGTGGATTTCGTTGTAGCTGGATCG 183 499 81 Antisense
AGGACAGATTTCACCGGGGGAGAGA 506 53 118 Antisense
GAAGAACTGTGGATCGTGGGTTGAT 598 339 240 Antisense
GAAGTCACCCTTAAAGCAACTCTCA 364 335 289 Antisense
CAAGGGCAAGAATGTCGTCCTGGGC 106 183 312 Antisense
TCGTCCTGGGCCCAGTTTTTGTTAG 354 603 326 Antisense
TATGCTAGCATTTTCGAGGATTCGA 460 655 364 Antisense
GCAAACTTCGGTTGTCCAGGTTCGA 185 317 393 Antisense
Sequence Source Affymetrix Proprietary Database