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[177663_at]

Description F35H8.4 /REP_DB=WormBase Gene ID /WP=CE00982 /TR=Q20083 /GB=CAA85326.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 16      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 13
(-3)
A 		47.6
(-0.3)
A 		22.5
(-2.3)
A 		90.8
(3)
A 		78
B 36.5
(-1.2)
P 		27.1
(-3)
A 		59.3
(3)
A 		41.2
(-0.4)
A 		32
C 22.2
(-3)
A 		34.4
(-2.1)
A 		39
(-1.7)
A 		102.4
(3)
A 		80
avr 23.9
(-3) 		36.4
(-1.6) 		40.3
(-1.2) 		78.1
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 17.2
(-3)
A 		19.1
(-2.9)
A 		20
(-2.8)
A 		24.6
(-2.6)
A 		93.2
(1.2)
P 		126.9
(3)
P 		110
B 32.5
(-1.3)
A 		19.1
(-2.1)
A 		3.8
(-3)
A 		14.1
(-2.4)
A 		63.4
(0.6)
P 		103.7
(3)
P 		100
C 17.9
(-3)
A 		57.8
(-1.6)
P 		34.4
(-2.4)
M 		75.6
(-1)
A 		141.8
(1.4)
P 		188.1
(3)
P 		170
avr 22.5
(-2.8) 		32
(-2.4) 		19.4
(-3) 		38.1
(-2.1) 		99.5
(1) 		139.6
(3) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 4.8
(-3)
A 		10.3
(0.9)
A 		5.9
(-2.2)
A 		13.3
(3)
A 		9
B 11.6
(1.2)
P 		6.7
(-3)
A 		13.7
(3)
A 		8.8
(-1.2)
A 		7
C 7.3
(-3)
A 		9.9
(-1.4)
A 		13.6
(0.8)
A 		17.3
(3)
A 		10
avr 7.9
(-3) 		9
(-1.7) 		11.1
(0.7) 		13.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 3.2
(-3)
A 		6.8
(-2.2)
A 		4.4
(-2.7)
A 		6.2
(-2.4)
A 		28.1
(2.4)
P 		30.9
(3)
P 		28
B 11.3
(-1.3)
A 		6.4
(-2.1)
A 		0.9
(-3)
A 		3.7
(-2.6)
A 		25.8
(1)
P 		38.7
(3)
P 		38
C 6.7
(-3)
A 		17.6
(-1.8)
P 		8.8
(-2.8)
M 		15.2
(-2.1)
A 		45.9
(1.2)
P 		63
(3)
P 		56
avr 7.1
(-2.6) 		10.3
(-2.1) 		4.7
(-3) 		8.4
(-2.4) 		33.3
(1.3) 		44.2
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177663_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3941
Sequence Type Exemplar sequence
Representative Public ID F35H8.4 NCBI
Target Description F35H8.4 /REP_DB=WormBase Gene ID /WP=CE00982 /TR=Q20083 /GB=CAA85326.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063655(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063655 NCBI Caenorhabditis elegans F35H8.4 (F35H8.4) mRNA, complete cds. 9/11 None
SNAP00000035510 ENSEMBL cdna:SNAP chromosome:CEL140:II:9559444:9559850:-1 9/11 None
F35H8.4 ENSEMBL cdna:known chromosome:CEL140:II:9559444:9559850:-1 gene:F35H8.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035514 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9559440-9559847(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H8.4
Entrez Gene 185326 Entrez gene
SwissProt Q20083 EMBL-EBI
WormBase CE35868 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA85326 Hypothetical protein F35H8.4 [Caenorhabditis elegans] ref|NP_496056.2| F35H8.4 [Caenorhabditis elegans] 2.0E-52
blast CAE60281 Hypothetical protein CBG03864 [Caenorhabditis briggsae] 6.0E-27
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496056.2 1 29-51
F35H8.4 1 29-51
SNAP00000035510 1 29-51
Sequence
Target Sequence 
>C. ELEGANS:177663_AT
caaccaatgtaacgatgatgtcagtgaaaagtttaacgactacggaatcagatattgagg
ttgaaaattttctagtttttcgaattattgatccttgggggtttcaggatggtccaaacc
caacgccaccatatgtctacatccttatcgaagtcggatacatcgttttgttcgtaatag
tgacgtatttgatgagaaaatacaactttatgaagacagacgatgacgcagttacaaaac
accatcgtcgccattttgaaatgatgaaacgtcagcaggaagatttgatcaccaggcaga
aatccaatgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACCAATGTAACGATGATGTCAGT 405 185 20 Antisense
GTTTAACGACTACGGAATCAGATAT 185 447 50 Antisense
TTCGAATTATTGATCCTTGGGGGTT 372 683 98 Antisense
TCCTTGGGGGTTTCAGGATGGTCCA 388 579 111 Antisense
GCCACCATATGTCTACATCCTTATC 123 281 144 Antisense
ATGTCTACATCCTTATCGAAGTCGG 313 49 152 Antisense
AAGTCGGATACATCGTTTTGTTCGT 586 153 170 Antisense
GAAGACAGACGATGACGCAGTTACA 91 341 231 Antisense
ACACCATCGTCGCCATTTTGAAATG 320 109 258 Antisense
GCAGGAAGATTTGATCACCAGGCAG 331 309 294 Antisense
GATCACCAGGCAGAAATCCAATGAG 230 421 306 Antisense
Sequence Source Affymetrix Proprietary Database