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[177646_at]

Description ZK809.5 /REP_DB=WormBase Gene ID /WP=CE03833 /TR=Q23600 /GB=CAA92639.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 76      4grp_high_8_8 > 19      6grp_highest_nonorm > 82      6grp_total > 66      4grp_total > 20      6grp_highest_sub > Da      6grp_high1_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 280
1 2 3 4 range
A 338.1
(-2.4)
P 		317.9
(-3)
P 		401.4
(-0.5)
A 		518.9
(3)
P 		201
B 356.4
(0)
P 		386.6
(3)
A 		325.6
(-3)
M 		364.6
(0.8)
P 		61
C 371.1
(3)
P 		346.4
(1.9)
A 		239
(-3)
P 		296.6
(-0.4)
P 		132
avr 355.2
(-0.2) 		350.3
(-0.6) 		322
(-3) 		393.4
(3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 348.3
(-2)
P 		324.7
(-3)
P 		336.4
(-2.5)
P 		384.7
(-0.6)
P 		364.7
(-1.4)
P 		473.1
(3)
P 		148
B 377.9
(-2.5)
P 		374.7
(-2.7)
P 		413
(-0.4)
P 		370.3
(-3)
P 		370.4
(-3)
P 		467.4
(3)
P 		97
C 295
(-3)
P 		376
(0.2)
P 		328.7
(-1.7)
P 		375.8
(0.2)
P 		320.6
(-2)
P 		447.3
(3)
P 		152
avr 340.4
(-3) 		358.5
(-2.1) 		359.4
(-2.1) 		376.9
(-1.2) 		351.9
(-2.4) 		462.6
(3) 		122
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 123.1
(3)
P 		69
(-3)
P 		104.9
(1)
A 		76
(-2.2)
P 		54
B 113.7
(3)
P 		95.5
(0.1)
A 		75.4
(-3)
M 		77.7
(-2.6)
P 		38
C 122.8
(3)
P 		99.6
(1.1)
A 		83.3
(-0.3)
P 		50
(-3)
P 		73
avr 119.9
(3) 		88
(-0.7) 		87.9
(-0.7) 		67.9
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 64.2
(-3)
P 		115.5
(3)
P 		73.3
(-1.9)
P 		97.6
(0.9)
P 		110.1
(2.4)
P 		115.2
(3)
P 		51
B 131.5
(-0.4)
P 		124.7
(-0.9)
P 		100.3
(-2.8)
P 		98.1
(-3)
P 		150.5
(1.1)
P 		174.3
(3)
P 		76
C 109.3
(-0.3)
P 		114.7
(0.2)
P 		83.7
(-2.3)
P 		75.6
(-3)
P 		103.8
(-0.7)
P 		149.7
(3)
P 		74
avr 101.7
(-1.4) 		118.3
(0.2) 		85.8
(-3) 		90.4
(-2.5) 		121.5
(0.5) 		146.4
(3) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177646_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8489
Sequence Type Exemplar sequence
Representative Public ID ZK809.5 NCBI
Target Description ZK809.5 /REP_DB=WormBase Gene ID /WP=CE03833 /TR=Q23600 /GB=CAA92639.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028454(11), NM_001028453(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028454 NCBI Caenorhabditis elegans ZK809.5b (ZK809.5) mRNA, complete cds. 11/11 None
NM_001028453 NCBI Caenorhabditis elegans ZK809.5a (ZK809.5) mRNA, complete cds. 11/11 None
SNAP00000011188 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11656727:11659169:1 11/11 None
GENEFINDER00000011198 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11656289:11659169:1 11/11 None
ZK809.5b ENSEMBL cdna:known chromosome:CEL140:IV:11656700:11659368:1 gene:ZK809.5 11/11 None
ZK809.5a ENSEMBL cdna:known chromosome:CEL140:IV:11656727:11659169:1 gene:ZK809.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11656294-11659175(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK809.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD89754 Hypothetical protein ZK809.5b [Caenorhabditis elegans] ref|NP_001023625.1| ZK809.5b [Caenorhabditis elegans] 0.0
blast CAA92639 Hypothetical protein ZK809.5a [Caenorhabditis elegans] ref|NP_001023624.1| ZK809.5a [Caenorhabditis elegans] 0.0
blast CAE62077 Hypothetical protein CBG06100 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 2.4E-5
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 2.4E-5
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 2.4E-5
Sequence
Target Sequence 
>C. ELEGANS:177646_AT
gagggattcgagtgatttctccacctgcaatgaaacgaagtcctgtagtcagaccagaaa
gtcccgaagttattctagaatctgaaaaaattgaagtgaaaattgagcagccgccggtga
agacaattgagccggaactgacggaatttgaaaaacgtatgcaagctcgtagtgcagcga
aagatgatacagatcggaggaatcgaattcttcaacttgccacggatccggaaacttcca
tcgtaaaaccgaaatcacctgaaccaacagaggaacctgcaaaaccaacgtctgctgata
atccgatgttcgccggagttgatcctgttatggccgagtacatgaagaaagttcttgcgg
ccagaaatcaaaatcaaacaactttgtctgctcaaccaaccatccaagtcgctccagtcg
tcgttccaccgaaaagcacagaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGGATTCGAGTGATTTCTCCACC 394 401 1736 Antisense
GATTTCTCCACCTGCAATGAAACGA 456 427 1749 Antisense
AACGAAGTCCTGTAGTCAGACCAGA 392 103 1769 Antisense
GCAGCCGCCGGTGAAGACAATTGAG 118 315 1842 Antisense
GAATCGAATTCTTCAACTTGCCACG 452 327 1935 Antisense
CCACGGATCCGGAAACTTCCATCGT 197 273 1955 Antisense
GAACCTGCAAAACCAACGTCTGCTG 156 347 2008 Antisense
GTCTGCTGATAATCCGATGTTCGCC 255 471 2025 Antisense
GGAGTTGATCCTGTTATGGCCGAGT 35 517 2050 Antisense
AAACAACTTTGTCTGCTCAACCAAC 263 129 2111 Antisense
GTCGTTCCACCGAAAAGCACAGAAG 10 473 2155 Antisense
Sequence Source Affymetrix Proprietary Database