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[177645_at] 7TM receptor, sre family

Description T13F2.5 /REP_DB=WormBase Gene ID /WP=CE13627 /TR=Q94048 /GB=CAB03356.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 05      4grp_highest_8_8 > 61      4grp_high_8_8 > 18      6grp_total > 15      4grp_total > 30      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 290
1 2 3 4 range
A 363.3
(3)
P 		154.9
(-1.4)
P 		79.8
(-3)
P 		259.8
(0.8)
P 		284
B 264.7
(3)
P 		159.4
(-1.1)
P 		111.5
(-3)
P 		227
(1.5)
P 		153
C 370.1
(3)
P 		189.1
(-1.2)
P 		113.7
(-3)
P 		324.2
(1.9)
P 		256
avr 332.7
(3) 		167.8
(-1.3) 		101.7
(-3) 		270.3
(1.4) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 262
(3)
P 		182.5
(0.4)
P 		183.5
(0.5)
P 		134.5
(-1.1)
P 		136.6
(-1)
P 		75.8
(-3)
A 		186
B 210.8
(2.9)
P 		211.8
(3)
P 		176.7
(1.1)
P 		112.9
(-2.5)
P 		140.9
(-0.9)
P 		103.4
(-3)
P 		108
C 241
(3)
P 		189.1
(0.9)
P 		122.2
(-1.9)
P 		153.7
(-0.6)
P 		98.2
(-2.9)
P 		95.8
(-3)
P 		145
avr 237.9
(3) 		194.5
(1.2) 		160.8
(-0.2) 		133.7
(-1.3) 		125.2
(-1.6) 		91.7
(-3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 132.3
(3)
P 		33.6
(-2.3)
P 		20.9
(-3)
P 		38
(-2.1)
P 		111
B 84.4
(3)
P 		39.4
(-1.6)
P 		25.8
(-3)
P 		48.4
(-0.7)
P 		59
C 122.5
(3)
P 		54.4
(-1.9)
P 		39.6
(-3)
P 		54.7
(-1.9)
P 		83
avr 113.1
(3) 		42.5
(-2) 		28.8
(-3) 		47
(-1.7) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 48.3
(0.9)
P 		64.9
(3)
P 		40
(-0.2)
P 		34.1
(-1)
P 		41.2
(-0.1)
P 		18.5
(-3)
A 		46
B 73.4
(3)
P 		70.5
(2.6)
P 		42.9
(-1.2)
P 		29.9
(-3)
P 		57.2
(0.8)
P 		38.6
(-1.8)
P 		44
C 89.3
(3)
P 		57.7
(-0.2)
P 		31.1
(-3)
P 		30.9
(-3)
P 		31.8
(-2.9)
P 		32.1
(-2.9)
P 		58
avr 70.3
(3) 		64.4
(2.1) 		38
(-1.8) 		31.6
(-2.7) 		43.4
(-1) 		29.7
(-3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177645_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8853
Sequence Type Exemplar sequence
Representative Public ID T13F2.5 NCBI
Target Description T13F2.5 /REP_DB=WormBase Gene ID /WP=CE13627 /TR=Q94048 /GB=CAB03356.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069345(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069345 NCBI Caenorhabditis elegans Serpentine Receptor, class E (epsilon) family member (sre-4) (sre-4) mRNA, complete cds. 11/11 None
GENEFINDER00000043485 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9781288:9786064:-1 11/11 None
T13F2.5 ENSEMBL cdna:known chromosome:CEL140:IV:9781017:9784652:-1 gene:T13F2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9781629-9784562(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM receptor, sre family
Gene Symbol sre-4
Ensembl T13F2.5
Entrez Gene 188477 Entrez gene
SwissProt Q94048 EMBL-EBI
WormBase CE38108 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7606 sensory perception of chemical stimulus inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03356 Hypothetical protein T13F2.5 [Caenorhabditis elegans] ref|NP_501746.3| Serpentine Receptor, class E (epsilon) family member (sre-4) [Caenorhabditis elegans] 0.0
blast CAB03356 Hypothetical protein T13F2.5 [Caenorhabditis elegans] ref|NP_501746.3| Serpentine Receptor, class E (epsilon) family member (sre-4) [Caenorhabditis elegans] 1.0E-150
blast CAE69016 Hypothetical protein CBG15014 [Caenorhabditis briggsae] 1.0E-113
blast CAE69016 Hypothetical protein CBG15014 [Caenorhabditis briggsae] 1.0E-102
blast CAE69018 Hypothetical protein CBG15016 [Caenorhabditis briggsae] 6.0E-42
blast CAE69018 Hypothetical protein CBG15016 [Caenorhabditis briggsae] 1.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR004151 EMBL-EBI C. elegans Sre G protein-coupled chemoreceptor 3.4E-21
Pfam IPR004151 EMBL-EBI C. elegans Sre G protein-coupled chemoreceptor 2.5E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501746.3 7 41-63,75-97,112-134,155-177,181-203,245-264,274-296
T13F2.5 7 41-63,75-97,112-134,155-177,181-203,245-264,274-296
GENEFINDER00000043485 6 84-106,121-143,164-186,190-212,254-273,283-305
Sequence
Target Sequence 
>C. ELEGANS:177645_AT
tggtgtattcttttttgttctttgcactaacagtcaatatcatccaaatttaacatggct
tttaaaaaatctagttgctcaatatttcttctcgatggcatgccgcctatttcaaattta
ttttcaatatggaattgacgatgaaagcggtttaaaatcaaattggtttttcatttttgt
tacattcagtaggaattgcttgatattcactgctctctatttttccccgtttgtcatgat
ggagagattgtatgcttctattcacattggagactacgagaacaaaccacgtcattatgt
tggctatacgctttctctatttttgtatctttggtctctaatgataggattaacatacgc
ctttgaaatcatacctacttacattcacgtatcctgcttggtatctatcaacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTGTATTCTTTTTTGTTCTTTGC 220 553 84 Antisense
GTTGCTCAATATTTCTTCTCGATGG 492 439 157 Antisense
TCGATGGCATGCCGCCTATTTCAAA 78 603 175 Antisense
TGATATTCACTGCTCTCTATTTTTC 220 565 284 Antisense
TATTTTTCCCCGTTTGTCATGATGG 374 661 301 Antisense
GAGATTGTATGCTTCTATTCACATT 391 397 327 Antisense
TATGTTGGCTATACGCTTTCTCTAT 103 657 379 Antisense
TCTATTTTTGTATCTTTGGTCTCTA 199 615 399 Antisense
ATACGCCTTTGAAATCATACCTACT 476 23 438 Antisense
ACCTACTTACATTCACGTATCCTGC 330 85 456 Antisense
GTATCCTGCTTGGTATCTATCAACC 339 455 472 Antisense
Sequence Source Affymetrix Proprietary Database