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[177642_at]

Description K03D3.1 /REP_DB=WormBase Gene ID /WP=CE16207 /TR=O45639 /GB=CAB05238.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 23      4grp_total > 27      6grp_cgc > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 10.5
(-1.8)
A 		31.5
(3)
A 		5.5
(-3)
A 		16.4
(-0.5)
A 		26
B 29.9
(1.2)
A 		38.4
(2.7)
A 		40.5
(3)
A 		4.5
(-3)
A 		36
C 30.9
(-1.3)
A 		21
(-2.3)
A 		13.1
(-3)
A 		77.6
(3)
A 		65
avr 23.8
(-1.1) 		30.3
(1.9) 		19.7
(-3) 		32.8
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 32.7
(3)
A 		2.1
(-3)
A 		30.9
(2.6)
A 		25.1
(1.5)
A 		31
(2.7)
A 		8
(-1.8)
A 		31
B 21.4
(-0.6)
A 		26.6
(1)
A 		33.3
(3)
A 		25.4
(0.6)
A 		13.4
(-3)
A 		16.1
(-2.2)
A 		20
C 33
(3)
A 		31.1
(2.6)
A 		8.1
(-2.3)
A 		5
(-3)
A 		6.9
(-2.6)
A 		10.6
(-1.8)
A 		28
avr 29
(3) 		19.9
(-0.1) 		24.1
(1.3) 		18.5
(-0.6) 		17.1
(-1.1) 		11.6
(-3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 3.8
(-0.3)
A 		6.8
(3)
A 		1.4
(-3)
A 		2.4
(-1.9)
A 		5
B 9.5
(3)
A 		9.5
(3)
A 		9.4
(2.9)
A 		1
(-3)
A 		9
C 10.2
(1)
A 		6
(-2)
A 		4.6
(-3)
A 		13.1
(3)
A 		9
avr 7.8
(3) 		7.4
(2.1) 		5.1
(-3) 		5.5
(-2.1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 6
(0.7)
A 		0.7
(-3)
A 		6.7
(1.1)
A 		6.4
(0.9)
A 		9.4
(3)
A 		1.9
(-2.2)
A 		9
B 7.5
(0.6)
A 		8.9
(3)
A 		8.1
(1.6)
A 		6.7
(-0.8)
A 		5.4
(-3)
A 		6
(-2)
A 		4
C 12.2
(3)
A 		9.5
(1.6)
A 		2.1
(-2.4)
A 		1
(-3)
A 		2.2
(-2.4)
A 		3.6
(-1.6)
A 		11
avr 8.6
(3) 		6.4
(0.3) 		5.6
(-0.8) 		4.7
(-1.9) 		5.7
(-0.6) 		3.8
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177642_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8864
Sequence Type Exemplar sequence
Representative Public ID K03D3.1 NCBI
Target Description K03D3.1 /REP_DB=WormBase Gene ID /WP=CE16207 /TR=O45639 /GB=CAB05238.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070544(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070544 NCBI Caenorhabditis elegans Serpentine Receptor, class Z family member (srz-74) (srz-74) mRNA, complete cds. 11/11 None
K03D3.1 ENSEMBL cdna:known chromosome:CEL140:IV:16329420:16331020:1 gene:K03D3.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040355 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16329425-16331026(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srz-74
Ensembl K03D3.1
Entrez Gene 186932 Entrez gene
SwissProt O45639 EMBL-EBI
WormBase CE16207 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05238 Hypothetical protein K03D3.1 [Caenorhabditis elegans] ref|NP_502945.1| Serpentine Receptor, class Z family member (srz-74) [Caenorhabditis elegans] 1.0E-169
blast AAC25815 Serpentine receptor, class z protein 79 [Caenorhabditis elegans] ref|NP_500359.1| Serpentine Receptor, class Z family member (srz-79) [Caenorhabditis elegans] 3.0E-72
blast B88650 protein C09G12.4 [imported] - Caenorhabditis elegans 2.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 4.1E-105
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502945.1 7 24-46,91-113,117-136,157-174,189-211,232-254,264-286
K03D3.1 7 24-46,91-113,117-136,157-174,189-211,232-254,264-286
Sequence
Target Sequence 
>C. ELEGANS:177642_AT
tacctcctcactgaagcatttcacctgatcacatttcttcttgcacttcaaagattcctg
atctactttttcccgccaggcgccgaaaaatatgttacggctgtccaagactttatcaac
aagcaaatttggaagctctatttagtaatgtttttaaaggaggttgtactaattatagtg
tattcgcttaccgagtttaacaaggaagaaggtctcacacattttggaatctattttatt
acagttgtattggttctttatgcattgttgtttctgtctacagtattctacataccaata
ataataagcgctaggagactgtcacatgtggattctgctcagcagtgtatggtccaaaag
tatatctactggcagacattgacagttttcatatgcaaattgcaatccgtctttctgtgc
atttaccttttcataagggccagttctatcaccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCTCCTCACTGAAGCATTTCACC 537 649 346 Antisense
TCACCTGATCACATTTCTTCTTGCA 111 627 366 Antisense
ATATGTTACGGCTGTCCAAGACTTT 391 21 435 Antisense
GAAGGTCTCACACATTTTGGAATCT 417 335 553 Antisense
ATGCATTGTTGTTTCTGTCTACAGT 477 43 605 Antisense
ATAAGCGCTAGGAGACTGTCACATG 545 25 649 Antisense
TGTCACATGTGGATTCTGCTCAGCA 606 557 665 Antisense
TCTGCTCAGCAGTGTATGGTCCAAA 641 599 679 Antisense
TGCAAATTGCAATCCGTCTTTCTGT 262 577 739 Antisense
CTTTCTGTGCATTTACCTTTTCATA 339 223 756 Antisense
TCATAAGGGCCAGTTCTATCACCGA 144 619 776 Antisense
Sequence Source Affymetrix Proprietary Database