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[177628_s_at]

Description ZK858.5 /REP_DB=WormBase Gene ID /WP=CE15429 /TR=Q94421 /GB=CAB02140.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 73      4grp_high_8_8 > 03      6grp_highest_nonorm > 71      6grp_total > 74      4grp_total > 20      6grp_high1_sub > 03_04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 420
1 2 3 4 range
A 368
(3)
P 		237.4
(-1.4)
P 		188.4
(-3)
A 		338.1
(2)
P 		180
B 344.4
(-0.8)
P 		324.7
(-1.1)
A 		196.1
(-3)
A 		608.7
(3)
P 		413
C 385
(-0.5)
P 		342.2
(-1.5)
A 		278.3
(-3)
P 		529.5
(3)
P 		251
avr 365.8
(0.2) 		301.4
(-1.2) 		220.9
(-3) 		492.1
(3) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 334
1 2 3 4 5 6 range
A 181.8
(-3)
P 		362.2
(0.2)
P 		348.3
(-0)
P 		516.2
(3)
P 		316.2
(-0.6)
P 		296.7
(-0.9)
P 		334
B 281.8
(-3)
P 		340.8
(2.1)
P 		308.1
(-0.7)
P 		315.7
(-0.1)
P 		351.3
(3)
P 		313.3
(-0.3)
P 		70
C 332.6
(-1.8)
P 		296.9
(-3)
P 		394.5
(0.4)
P 		299.4
(-2.9)
P 		305
(-2.7)
P 		468.9
(3)
P 		172
avr 265.4
(-3) 		333.3
(0.6) 		350.3
(1.6) 		377.1
(3) 		324.2
(0.2) 		359.6
(2.1) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 134
(3)
P 		51.5
(-2.8)
P 		49.3
(-3)
A 		49.5
(-3)
P 		85
B 109.8
(1.6)
P 		80.2
(-0.5)
A 		45.4
(-3)
A 		129.7
(3)
P 		84
C 127.4
(3)
P 		98.4
(-1.6)
A 		97
(-1.8)
P 		89.3
(-3)
P 		38
avr 123.7
(3) 		76.7
(-1.7) 		63.9
(-3) 		89.5
(-0.4) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 33.5
(-3)
P 		128.8
(2.9)
P 		75.9
(-0.4)
P 		130.9
(3)
P 		95.4
(0.8)
P 		72.2
(-0.6)
P 		97
B 98.1
(-0.9)
P 		113.4
(0.4)
P 		74.8
(-3)
P 		83.6
(-2.2)
P 		142.7
(3)
P 		116.8
(0.7)
P 		68
C 123.3
(0.9)
P 		90.6
(-1.1)
P 		100.4
(-0.5)
P 		60.2
(-3)
P 		98.7
(-0.6)
P 		157
(3)
P 		97
avr 85
(-2.8) 		110.9
(2.2) 		83.7
(-3) 		91.6
(-1.5) 		112.3
(2.4) 		115.3
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177628_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1950
Sequence Type Exemplar sequence
Representative Public ID ZK858.5 NCBI
Target Description ZK858.5 /REP_DB=WormBase Gene ID /WP=CE15429 /TR=Q94421 /GB=CAB02140.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060049(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060049 NCBI Caenorhabditis elegans ZK858.5 (ZK858.5) mRNA, complete cds. 10/11 None
SNAP00000011877 ENSEMBL cdna:SNAP chromosome:CEL140:I:9143971:9146480:-1 11/11 None
GENEFINDER00000011887 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9143971:9146480:-1 10/11 None
ZK858.5 ENSEMBL cdna:known chromosome:CEL140:I:9143971:9146480:-1 gene:ZK858.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9143901-9146411(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK858.5
Entrez Gene 172738 Entrez gene
SwissProt Q94421 EMBL-EBI
WormBase CE34235 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02140 Hypothetical protein ZK858.5 [Caenorhabditis elegans] ref|NP_492450.2| ZK858.5 [Caenorhabditis elegans] 0.0
blast CAE67045 Hypothetical protein CBG12452 [Caenorhabditis briggsae] 1.0E-154
blast CAE67045 Hypothetical protein CBG12452 [Caenorhabditis briggsae] 1.0E-147
Protein Domains
Database ID Description E-Value
Pfam IPR007829 EMBL-EBI TM2 8.5E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492450.2 6 10-30,37-56,100-122,129-148,168-190,210-227
ZK858.5 6 10-30,37-56,100-122,129-148,168-190,210-227
SNAP00000011877 5 10-30,37-56,100-122,127-149,169-188
GENEFINDER00000011887 6 10-30,37-56,100-122,129-148,168-190,210-227
Sequence
Target Sequence 
>C. ELEGANS:177628_S_AT
atggccagtttctcatcaatgttcattatgttctggtcatcattattcctgatgctcgtt
tgtgtaatcctactgggatgctcgagaaaagttgcagataagcaggtaactgccactcgt
cctggtacattccgagaaactatatctgttggaagtctgattagagatcgtatttttgat
gcgaaaaaagttcacagttttttcgaaggaaatccaataattgaatatcattcaaaatcg
gatatcaaaaacaaaaatggagaaaaaacactaaaaaatagttcattttggtaccaagta
tggagtggagagcttttcgatgaactcactggagcagcacatctcacgaaaattgattgg
attgaattaacaactacattcatcgtagatgtactacggtctgaagctcgtgttattgat
ggatcttcaactattgaaccatttaaatgggcactttggcgtaattatcttattcatcgt
ttctcactggatccattgatttctgatgatcgcctccgcactgaatgcaaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCCAGTTTCTCATCAATGTTCA 516 53 496 Antisense
ATGTTCTGGTCATCATTATTCCTGA 151 47 523 Antisense
GATGCTCGTTTGTGTAATCCTACTG 677 409 546 Antisense
TAAGCAGGTAACTGCCACTCGTCCT 505 637 594 Antisense
CCACTCGTCCTGGTACATTCCGAGA 301 275 608 Antisense
GAGAGCTTTTCGATGAACTCACTGG 614 393 803 Antisense
CTCACTGGAGCAGCACATCTCACGA 208 229 820 Antisense
GTACTACGGTCTGAAGCTCGTGTTA 612 459 886 Antisense
TGGGCACTTTGGCGTAATTATCTTA 680 547 943 Antisense
CGTTTCTCACTGGATCCATTGATTT 632 247 973 Antisense
TCGCCTCCGCACTGAATGCAAAAAA 341 599 1005 Antisense
Sequence Source Affymetrix Proprietary Database