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[177606_at]

Description F25H2.4 /REP_DB=WormBase Gene ID /WP=CE09649 /TR=Q93568 /GB=CAB02093.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 98      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 88      6grp_total > 97      4grp_total > 48      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1116
1 2 3 4 range
A 936.5
(-3)
P 		1594.1
(0.5)
P 		2052.9
(3)
P 		1347.7
(-0.8)
P 		1116
B 1070.4
(-3)
P 		1739.1
(1.8)
P 		1900.3
(3)
P 		1414.8
(-0.5)
P 		830
C 960.8
(-3)
P 		1605.1
(2.4)
P 		1671.5
(3)
P 		1269.6
(-0.4)
P 		711
avr 989.2
(-3) 		1646.1
(1.5) 		1874.9
(3) 		1344
(-0.6) 		886

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 880
1 2 3 4 5 6 range
A 1383.1
(-0.3)
P 		1200.7
(-1.8)
P 		1062.3
(-3)
P 		1300.8
(-1)
P 		1527.3
(0.9)
P 		1773.7
(3)
P 		711
B 1221.9
(-0.8)
P 		1023.9
(-3)
P 		1224.2
(-0.8)
P 		1280.1
(-0.1)
P 		1453.1
(1.8)
P 		1558.3
(3)
P 		534
C 1059.7
(-2.2)
P 		939.7
(-3)
P 		1231.6
(-1)
P 		1308.9
(-0.5)
P 		1479.7
(0.7)
P 		1819.4
(3)
P 		880
avr 1221.6
(-1.5) 		1054.8
(-3) 		1172.7
(-1.9) 		1296.6
(-0.8) 		1486.7
(0.9) 		1717.1
(3) 		662
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 385
1 2 3 4 range
A 341
(-0.5)
P 		346
(-0.4)
P 		536.7
(3)
P 		197.3
(-3)
P 		339
B 341.3
(-1.3)
P 		429.5
(2.5)
P 		440.3
(3)
P 		301.5
(-3)
P 		139
C 318
(-1.3)
P 		461.5
(1)
P 		582.6
(3)
P 		214.1
(-3)
P 		369
avr 333.4
(-1) 		412.3
(0.7) 		519.9
(3) 		237.6
(-3) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 378
1 2 3 4 5 6 range
A 255
(-2.4)
P 		427
(2.1)
P 		231.3
(-3)
P 		329.9
(-0.4)
P 		460.9
(3)
P 		431.9
(2.2)
P 		230
B 425.2
(-0.4)
P 		340.8
(-2.1)
P 		297.3
(-3)
P 		339.1
(-2.1)
P 		590.4
(3)
P 		581.1
(2.8)
P 		293
C 392.8
(-0.8)
P 		286.7
(-2.6)
P 		313.5
(-2.1)
P 		263.2
(-3)
P 		478.8
(0.7)
P 		609.1
(3)
P 		346
avr 357.7
(-1.2) 		351.5
(-1.4) 		280.7
(-3) 		310.7
(-2.3) 		510
(2.3) 		540.7
(3) 		260
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177606_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2388
Sequence Type Exemplar sequence
Representative Public ID F25H2.4 NCBI
Target Description F25H2.4 /REP_DB=WormBase Gene ID /WP=CE09649 /TR=Q93568 /GB=CAB02093.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060359(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060359 NCBI Caenorhabditis elegans F25H2.4 (F25H2.4) mRNA, complete cds. 11/11 None
SNAP00000042617 ENSEMBL cdna:SNAP chromosome:CEL140:I:10560100:10561046:1 11/11 None
GENEFINDER00000042637 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10560100:10561046:1 11/11 None
F25H2.4 ENSEMBL cdna:known chromosome:CEL140:I:10560100:10561091:1 gene:F25H2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10560030-10560977(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H2.4
Entrez Gene 172938 Entrez gene
SwissProt Q93568 EMBL-EBI
WormBase CE09649 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02093 Hypothetical protein F25H2.4 [Caenorhabditis elegans] ref|NP_492760.1| F25H2.4 [Caenorhabditis elegans] 1.0E-99
blast CAE58973 Hypothetical protein CBG02246 [Caenorhabditis briggsae] 1.0E-92
blast AAL68373 SD01117p [Drosophila melanogaster] 2.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR005366 EMBL-EBI Protein of unknown function UPF0172 2.8E-15
Sequence
Target Sequence 
>C. ELEGANS:177606_AT
tcatccttcactgcctgaaatacccggcaaagggtgtttttggactgctgattggaaaca
agaaaggagacaaagtcacggtaacaagttgtgttcctctttgccatgaatccactccac
tcgcacctcctctggaacttgccacagccctcgtccatggaaagtttggtgcctccctcg
tcggcgtctatttctcaaatgcaactccatcggacacttctctcaacgtttatgccactc
gactcgccgacagaatatcttctgttacctcttcggcagcgattctcgtacaagtgatga
acgaaagactggtgtcagattgtgaacaagaccgcctcgtggcatatgaaaaagatggag
atagttggaaagaaacgaaaacgattttccaaggttccaacttcttgcgtggccttcaag
ctgttattcagaagaagttgtaccgagagctttccgatttcgaaaaccatttggacaatc
ccgagttcgacttcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCCTTCACTGCCTGAAATACCC 695 619 56 Antisense
CACAGCCCTCGTCCATGGAAAGTTT 418 195 198 Antisense
GTCGGCGTCTATTTCTCAAATGCAA 39 473 235 Antisense
AAATGCAACTCCATCGGACACTTCT 66 121 252 Antisense
TTCTCTCAACGTTTATGCCACTCGA 302 693 273 Antisense
TCGACTCGCCGACAGAATATCTTCT 281 601 294 Antisense
TCTTCGGCAGCGATTCTCGTACAAG 347 617 325 Antisense
AACAAGACCGCCTCGTGGCATATGA 344 135 380 Antisense
CCAAGGTTCCAACTTCTTGCGTGGC 181 273 444 Antisense
GTTGTACCGAGAGCTTTCCGATTTC 629 437 492 Antisense
TGGACAATCCCGAGTTCGACTTCTA 52 549 527 Antisense
Sequence Source Affymetrix Proprietary Database