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[177582_s_at]

Description F52G2.2 /REP_DB=WormBase Gene ID /WP=CE17848 /TR=Q9XUE3 /GB=CAB05207.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 67      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 83      6grp_total > 68      4grp_total > 14      6grp_highest_sub > Da      6grp_cgc > 39      6grp_high1_sub > Da      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 229
1 2 3 4 range
A 323.4
(0.9)
P 		221.2
(-2)
P 		187.7
(-3)
P 		396.7
(3)
P 		209
B 364.6
(3)
P 		168.2
(-3)
P 		193.3
(-2.2)
P 		352.2
(2.6)
P 		196
C 371.7
(3)
P 		224.6
(-3)
P 		241
(-2.3)
P 		366.8
(2.8)
P 		147
avr 353.2
(2.3) 		204.7
(-3) 		207.3
(-2.9) 		371.9
(3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 375
1 2 3 4 5 6 range
A 326.8
(-2.5)
P 		382.9
(-1.6)
P 		297.9
(-3)
P 		404.5
(-1.3)
P 		372.1
(-1.8)
P 		673
(3)
P 		375
B 369.8
(-1.6)
P 		362.9
(-1.8)
P 		297.9
(-3)
P 		367.8
(-1.7)
P 		353.4
(-1.9)
P 		614.9
(3)
P 		317
C 506.1
(2.6)
P 		350.5
(-3)
P 		366.7
(-2.4)
P 		350.7
(-3)
P 		418.7
(-0.5)
P 		516.4
(3)
P 		166
avr 400.9
(-1.3) 		365.4
(-2) 		320.8
(-3) 		374.3
(-1.9) 		381.4
(-1.7) 		601.4
(3) 		281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 117.7
(3)
P 		48
(-3)
P 		49.1
(-2.9)
P 		58.1
(-2.1)
P 		70
B 116.3
(3)
P 		41.5
(-3)
P 		44.8
(-2.7)
P 		75
(-0.3)
P 		75
C 123
(3)
P 		64.6
(-2.7)
P 		84
(-0.8)
P 		61.9
(-3)
P 		61
avr 119
(3) 		51.4
(-3) 		59.3
(-2.3) 		65
(-1.8) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 60.2
(-3)
P 		136.2
(1.4)
P 		64.9
(-2.7)
P 		102.6
(-0.5)
P 		112.3
(0)
P 		163.8
(3)
P 		104
B 128.7
(-0.8)
P 		120.8
(-1.1)
P 		72.3
(-3)
P 		97.4
(-2)
P 		143.6
(-0.3)
P 		229.3
(3)
P 		157
C 187.6
(3)
P 		106.9
(-1.1)
P 		93.3
(-1.8)
P 		70.5
(-3)
P 		135.5
(0.3)
P 		172.9
(2.2)
P 		117
avr 125.5
(-0.4) 		121.3
(-0.6) 		76.8
(-3) 		90.2
(-2.3) 		130.5
(-0.1) 		188.7
(3) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177582_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9378
Sequence Type Exemplar sequence
Representative Public ID F52G2.2 NCBI
Target Description F52G2.2 /REP_DB=WormBase Gene ID /WP=CE17848 /TR=Q9XUE3 /GB=CAB05207.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070209(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070209 NCBI Caenorhabditis elegans RNAi Spreading Defective (see also sid) family member (rsd-2) (rsd-2) mRNA, complete cds. 11/11 None
SNAP00000029679 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13516541:13526239:-1 11/11 None
GENEFINDER00000029686 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13522193:13532770:-1 11/11 None
F52G2.2 ENSEMBL cdna:known chromosome:CEL140:IV:13521938:13539584:-1 gene:F52G2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13522069-13539590(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rsd-2
Ensembl F52G2.2
Entrez Gene 178322 Entrez gene
SwissProt Q9XUE3 EMBL-EBI
WormBase CE17848 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05207 Hypothetical protein F52G2.2 [Caenorhabditis elegans] ref|NP_502610.1| RNAi Spreading Defective (see also sid) family member (rsd-2) [Caenorhabditis elegans] 0.0
blast AAZ73241 RNA-decapping protein [Caenorhabditis elegans] 0.0
blast CAE58589 Hypothetical protein CBG01756 [Caenorhabditis briggsae] 1.0E-178
blast CAE58588 Hypothetical protein CBG01755 [Caenorhabditis briggsae] 2.0E-30
blast CAE58588 Hypothetical protein CBG01755 [Caenorhabditis briggsae] 3.0E-30
blast CAB05205 Hypothetical protein F52G2.3 [Caenorhabditis elegans] ref|NP_502611.2| F52G2.3 [Caenorhabditis elegans] 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR007722 EMBL-EBI Dcp2, box A 2.3E-65
Pfam IPR000086 EMBL-EBI NUDIX hydrolase 2.7E-19
Sequence
Target Sequence 
>C. ELEGANS:177582_S_AT
agcagcagcaatcgtcgttgtcttcgagaggacctacgagaccatattctccagtcggca
gttatcaaagttctcatcaaaactttgataatcgttcggcggcacataatggagatggta
gatcaacatcgcaaagtcatcattctgcaggaggatctatgaatcatcaaaacttttatc
agccgccagtttatcagtcgagtagctcacagcagagctatcaaccgagatataacggag
caccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGCAGCAATCGTCGTTGTCTTC 206 77 3302 Antisense
GTCGTTGTCTTCGAGAGGACCTACG 198 415 3315 Antisense
GAGGACCTACGAGACCATATTCTCC 2 401 3329 Antisense
CATATTCTCCAGTCGGCAGTTATCA 375 215 3344 Antisense
GATAATCGTTCGGCGGCACATAATG 187 423 3388 Antisense
GGTAGATCAACATCGCAAAGTCATC 150 505 3418 Antisense
AAGTCATCATTCTGCAGGAGGATCT 47 159 3435 Antisense
TATCAGCCGCCAGTTTATCAGTCGA 351 659 3478 Antisense
TTATCAGTCGAGTAGCTCACAGCAG 100 681 3492 Antisense
CTCACAGCAGAGCTATCAACCGAGA 349 227 3507 Antisense
AACCGAGATATAACGGAGCACCATC 278 147 3524 Antisense
Sequence Source Affymetrix Proprietary Database