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[177581_at]

Description F45G2.5 /REP_DB=WormBase Gene ID /WP=CE16048 /TR=O62247 /GB=CAB07614.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 72      4grp_total > 57      6grp_cgc > 75      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 482
1 2 3 4 range
A 4.2
(-3)
A 		375.5
(2.3)
P 		425.5
(3)
P 		53.7
(-2.3)
A 		421
B 2.4
(-3)
A 		344.2
(1.3)
P 		484.4
(3)
P 		28.2
(-2.7)
A 		482
C 6.3
(-3)
A 		372.4
(2.1)
P 		433.2
(3)
P 		28.3
(-2.7)
A 		427
avr 4.3
(-3) 		364
(1.9) 		447.7
(3) 		36.7
(-2.6) 		443

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 468
1 2 3 4 5 6 range
A 6.9
(-3)
A 		245.7
(0.1)
P 		474
(3)
P 		474.6
(3)
P 		318.7
(1)
P 		109.5
(-1.7)
A 		468
B 48.8
(-3)
A 		254.5
(0.6)
P 		365.7
(2.6)
P 		348.7
(2.3)
P 		388.8
(3)
P 		176.5
(-0.7)
P 		340
C 36
(-3)
A 		282.2
(0.7)
P 		263
(0.4)
P 		430.9
(3)
P 		332.3
(1.5)
P 		182.1
(-0.8)
P 		395
avr 30.6
(-3) 		260.8
(0.6) 		367.6
(2.2) 		418.1
(3) 		346.6
(1.9) 		156
(-1.1) 		388
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 1.5
(-3)
A 		81.5
(1.4)
P 		111.2
(3)
P 		7.9
(-2.6)
A 		110
B 0.8
(-3)
A 		85
(1.5)
P 		112.3
(3)
P 		6
(-2.7)
A 		112
C 2.1
(-3)
A 		107.1
(1.2)
P 		151
(3)
P 		4.8
(-2.9)
A 		149
avr 1.5
(-3) 		91.2
(1.4) 		124.8
(3) 		6.2
(-2.8) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 1.3
(-3)
A 		87.4
(1.3)
P 		103.2
(2.1)
P 		120.4
(3)
P 		96.2
(1.8)
P 		26.7
(-1.7)
A 		119
B 17
(-3)
A 		84.7
(-0.1)
P 		88.8
(0.1)
P 		92.3
(0.2)
P 		158
(3)
P 		65.8
(-0.9)
P 		141
C 13.3
(-3)
A 		86.1
(1.6)
P 		66.9
(0.4)
P 		86.7
(1.7)
P 		107.5
(3)
P 		61
(0)
P 		94
avr 10.5
(-3) 		86.1
(1.1) 		86.3
(1.1) 		99.8
(1.9) 		120.6
(3) 		51.2
(-0.8) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177581_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7437
Sequence Type Exemplar sequence
Representative Public ID F45G2.5 NCBI
Target Description F45G2.5 /REP_DB=WormBase Gene ID /WP=CE16048 /TR=O62247 /GB=CAB07614.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067371(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067371 NCBI Caenorhabditis elegans BLIstered cuticle family member (bli-5) (bli-5) mRNA, complete cds. 11/11 None
SNAP00000011670 ENSEMBL cdna:SNAP chromosome:CEL140:III:13427239:13429164:-1 10/11 None
GENEFINDER00000011679 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13427239:13429229:-1 11/11 None
F45G2.5 ENSEMBL cdna:known chromosome:CEL140:III:13427239:13429229:-1 gene:F45G2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13427234-13429225(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bli-5
Ensembl F45G2.5
Entrez Gene 185812 Entrez gene
SwissProt O62247 EMBL-EBI
WormBase CE16048 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07614 Hypothetical protein F45G2.5 [Caenorhabditis elegans] ref|NP_499772.1| BLIstered cuticle family member (bli-5) [Caenorhabditis elegans] 1.0E-125
blast CAB07614 Hypothetical protein F45G2.5 [Caenorhabditis elegans] ref|NP_499772.1| BLIstered cuticle family member (bli-5) [Caenorhabditis elegans] 1.0E-112
blast CAE73705 Hypothetical protein CBG21218 [Caenorhabditis briggsae] 1.0E-104
blast CAE73705 Hypothetical protein CBG21218 [Caenorhabditis briggsae] 1.0E-100
blast CAE62776 Hypothetical protein CBG06945 [Caenorhabditis briggsae] 7.0E-18
blast CAE62776 Hypothetical protein CBG06945 [Caenorhabditis briggsae] 8.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 5.2E-7
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 4.2E-5
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 8.6E-7
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 4.2E-5
Sequence
Target Sequence 
>C. ELEGANS:177581_AT
ggtatctatccataattcattcatcttattgatgttaatgatatcaatttgtttttgtga
gaaatgcctgaccaatgaagaatgcgatttgaaatggccagacgcaatatgtgttcgtgg
aagatgccgttgttctgagaatacaattcgaaagaaaagtgcatcaagagaatgggtttg
tttggcaactaatgatgcaaccggcaactcgggtcctccattgacatgtccaactccgga
aggagctggataccaagtaatgtaccgaaaagatggagaaccggtgaaatgttcgagtaa
aaagaagccagatacgtgtccagaaggatttgaatgtattcagggattatcaattcttgg
agcattggatggagtttgttgtcctgatagagccaaaacatgcgtccacccaatattcga
tcatccggatgatggatatctgtctagatggggattcgatggtgaacaatgtattgaatt
caaatggaatcccgaaaggccgtcatcggcaaacaatttcaaaactcgcgcacatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTATCTATCCATAATTCATTCATC 403 505 15 Antisense
GGCCAGACGCAATATGTGTTCGTGG 276 543 110 Antisense
GTGGAAGATGCCGTTGTTCTGAGAA 537 491 131 Antisense
AATGATGCAACCGGCAACTCGGGTC 332 163 205 Antisense
CTCCATTGACATGTCCAACTCCGGA 614 233 230 Antisense
GAAGCCAGATACGTGTCCAGAAGGA 126 345 318 Antisense
GGAGTTTGTTGTCCTGATAGAGCCA 327 517 385 Antisense
GATAGAGCCAAAACATGCGTCCACC 263 425 400 Antisense
CCACCCAATATTCGATCATCCGGAT 455 273 420 Antisense
AATGGAATCCCGAAAGGCCGTCATC 151 161 497 Antisense
AACAATTTCAAAACTCGCGCACATT 616 133 526 Antisense
Sequence Source Affymetrix Proprietary Database