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[177574_at]

Description F31C3.5 /REP_DB=WormBase Gene ID /WP=CE17734 /TR=O62193 /GB=CAB07195.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 49      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 100.4
(-3)
A 		238
(3)
P 		199.9
(1.3)
A 		100.8
(-3)
A 		138
B 100.4
(-3)
A 		176.1
(0.5)
A 		231.4
(3)
P 		129.7
(-1.7)
A 		131
C 101.5
(-3)
A 		194.1
(3)
A 		161.1
(0.9)
P 		108
(-2.6)
A 		93
avr 100.8
(-3) 		202.7
(3) 		197.5
(2.7) 		112.8
(-2.3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 452
1 2 3 4 5 6 range
A 90.8
(-3)
A 		113.7
(-2.7)
A 		121
(-2.6)
A 		141.6
(-2.3)
A 		324.1
(0.2)
P 		528.8
(3)
P 		438
B 135
(-2.5)
A 		101.6
(-3)
A 		111.4
(-2.9)
A 		157.6
(-2.1)
P 		389.9
(1.4)
P 		494.4
(3)
P 		393
C 82
(-2.9)
A 		77
(-3)
A 		154.1
(-1.9)
A 		181
(-1.5)
M 		297.5
(0.2)
P 		490.2
(3)
P 		413
avr 102.6
(-2.9) 		97.4
(-3) 		128.8
(-2.5) 		160.1
(-2.1) 		337.2
(0.5) 		504.5
(3) 		407
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 36.5
(0.5)
A 		51.7
(2.9)
P 		52.3
(3)
A 		14.7
(-3)
A 		38
B 32
(-2)
A 		43.5
(0.7)
A 		53.6
(3)
P 		27.6
(-3)
A 		26
C 33.6
(-0.6)
A 		55.8
(2.9)
A 		56.2
(3)
P 		18.2
(-3)
A 		38
avr 34
(-0.6) 		50.3
(2.3) 		54
(3) 		20.2
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 16.7
(-3)
A 		40.5
(-1.7)
A 		26.3
(-2.5)
A 		35.9
(-2)
A 		97.8
(1.3)
P 		128.7
(3)
P 		112
B 47
(-2.2)
A 		33.8
(-2.7)
A 		27
(-3)
A 		41.8
(-2.4)
P 		158.4
(2)
P 		184.4
(3)
P 		157
C 30.4
(-2.7)
A 		23.5
(-3)
A 		39.2
(-2.3)
A 		36.4
(-2.4)
M 		96.3
(0.1)
P 		164.1
(3)
P 		141
avr 31.4
(-3) 		32.6
(-2.9) 		30.8
(-3) 		38
(-2.7) 		117.5
(1.1) 		159.1
(3) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177574_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1324
Sequence Type Exemplar sequence
Representative Public ID F31C3.5 NCBI
Target Description F31C3.5 /REP_DB=WormBase Gene ID /WP=CE17734 /TR=O62193 /GB=CAB07195.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061226(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061226 NCBI Caenorhabditis elegans F31C3.5 (F31C3.5) mRNA, complete cds. 11/11 None
SNAP00000023488 ENSEMBL cdna:SNAP chromosome:CEL140:I:15061143:15061736:1 11/11 None
GENEFINDER00000023496 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:15061143:15061736:1 11/11 None
F31C3.5 ENSEMBL cdna:known chromosome:CEL140:I:15061143:15061736:1 gene:F31C3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:15061073-15061667(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31C3.5
Entrez Gene 185146 Entrez gene
SwissProt O62193 EMBL-EBI
WormBase CE17734 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256284_AT DNA replication protein-related at
CANINE_2:CFAAFFX.30492.1.S1_AT similar to DNA replication complex GINS protein PSF2 cfa
DROSOPHILA_2:1628599_AT dm
DROSGENOME1:145755_AT dm
HG-U133_PLUS_2:221521_S_AT DNA replication complex GINS protein PSF2 hs
HG-U133A_2:221521_S_AT DNA replication complex GINS protein PSF2 hs
HG-U133A:221521_S_AT DNA replication complex GINS protein PSF2 hs
HG-FOCUS:221521_S_AT DNA replication complex GINS protein PSF2 hs
HU35KSUBA:AA262870_AT DNA replication complex GINS protein PSF2 hs
U133_X3P:G13112024_3P_A_AT DNA replication complex GINS protein PSF2 hs
U133_X3P:HS2.429227.1.S1_3P_S_AT DNA replication complex GINS protein PSF2 hs
HG-U95C:48827_AT DNA replication complex GINS protein PSF2 hs
HG-U95E:73010_AT DNA replication complex GINS protein PSF2 hs
HG-U133_PLUS_2:1557595_AT DNA replication complex GINS protein PSF2 hs
HU35KSUBA:AA251723_AT DNA replication complex GINS protein PSF2 hs
MG-U74AV2:98932_AT RIKEN cDNA 4833427B12 gene mm
MOE430B:1428713_S_AT RIKEN cDNA 4833427B12 gene mm
MOUSE430_2:1428713_S_AT RIKEN cDNA 4833427B12 gene mm
MOE430B:1452881_AT RIKEN cDNA 4833427B12 gene mm
MOUSE430_2:1452881_AT RIKEN cDNA 4833427B12 gene mm
MU19KSUBB:TC27660_AT RIKEN cDNA 4833427B12 gene, mRNA (cDNA clone MGC:105165 IMAGE:30551463) mm
YEAST_2:1773385_AT Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which binds to DNA replication origins and facilitates assembly of the DNA replication machinery Sc
Protein Similarities
Method ID Description E-Value
blast CAB07195 Hypothetical protein F31C3.5 [Caenorhabditis elegans] ref|NP_493627.1| F31C3.5 [Caenorhabditis elegans] sp|O62193|PSF2_CAEEL Probable DNA replication complex GINS protein PSF2 7.0E-99
blast CAE72580 Hypothetical protein CBG19768 [Caenorhabditis briggsae] 1.0E-83
blast CAC70161 conserved hypothetical protein [Brugia malayi] sp|Q8IHI1|PSF2_BRUMA Probable DNA replication complex GINS protein PSF2 1.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR007257 EMBL-EBI Protein of unknown function DUF392 9.0E-105
Sequence
Target Sequence 
>C. ELEGANS:177574_AT
gaacgttgcgagttcatcgccggaaactcgcttattgaagtgatcccctcaatttccgat
gaccggccgattcacctgatttccggcgatattggtccatttgaggcaggcgtaccctgt
cggattccagtttggacagcgattttgatgaaacgaaaacacaattgcaaggtggtggct
cctcaatggatggatgtagatgagctgaagaagatattgacgtcagagactgaaagccaa
ggattggcgaaattaccggatcatttctttgaaatctcgcatatgcttgttcgagatgcc
cgtgaggatatattcgaagttgaagctgtcaaatcattggttcaggacatttatgatcgg
agggacgcaaaacttcgaagctcagcgatcgaattcttgagacagaaccaaacctgccac
gctcaattggacaacgttcagctgattgaagcatcgtccgctcgtgccactttggaggcc
tgtcgtcagatgggagctgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGTTGCGAGTTCATCGCCGGAA 86 349 22 Antisense
ATCGCCGGAAACTCGCTTATTGAAG 502 37 37 Antisense
CAATTTCCGATGACCGGCCGATTCA 301 181 71 Antisense
GATTCACCTGATTTCCGGCGATATT 216 431 90 Antisense
TGGTCCATTTGAGGCAGGCGTACCC 669 549 114 Antisense
TTGCAAGGTGGTGGCTCCTCAATGG 267 701 186 Antisense
TATGCTTGTTCGAGATGCCCGTGAG 6 657 303 Antisense
TGAGACAGAACCAAACCTGCCACGC 182 569 419 Antisense
CCTGCCACGCTCAATTGGACAACGT 331 267 434 Antisense
CAGCTGATTGAAGCATCGTCCGCTC 42 203 460 Antisense
GGCCTGTCGTCAGATGGGAGCTGTC 245 541 498 Antisense
Sequence Source Affymetrix Proprietary Database