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[177573_at]

Description E01G4.4 /REP_DB=WormBase Gene ID /WP=CE09111 /TR=O02238 /GB=CAB05716.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 18      4grp_total > 42      6grp_cgc > 01      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 5
(-3)
A 		35.4
(3)
A 		6.7
(-2.7)
A 		7.1
(-2.6)
A 		30
B 3.8
(-3)
A 		5.8
(-0.5)
A 		6
(-0.3)
A 		8.6
(3)
A 		5
C 3.2
(-0.6)
A 		2.6
(-3)
A 		4.1
(3)
A 		3.6
(1)
A 		2
avr 4
(-3) 		14.6
(3) 		5.6
(-2.1) 		6.4
(-1.6) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 57.8
(3)
A 		2.7
(-2.9)
A 		2.8
(-2.9)
A 		2.2
(-3)
A 		6.6
(-2.5)
A 		6.4
(-2.5)
A 		56
B 5.5
(2.6)
A 		2.3
(-3)
A 		2.9
(-1.9)
A 		2.7
(-2.3)
A 		5.3
(2.3)
A 		5.7
(3)
A 		3
C 3.9
(-2.7)
A 		7.1
(-0.6)
A 		5.2
(-1.9)
A 		12.6
(3)
A 		3.5
(-3)
A 		10.9
(1.9)
A 		9
avr 22.4
(3) 		4
(-2.9) 		3.6
(-3) 		5.8
(-2.3) 		5.1
(-2.5) 		7.7
(-1.7) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.8
(-2.3)
A 		7.7
(3)
A 		1.7
(-2.4)
A 		1
(-3)
A 		7
B 1.2
(-3)
A 		1.4
(-1)
A 		1.4
(-1)
A 		1.8
(3)
A 		1
C 1.1
(0.8)
A 		0.8
(-1.5)
A 		1.4
(3)
A 		0.6
(-3)
A 		1
avr 1.4
(-2.2) 		3.3
(3) 		1.5
(-1.9) 		1.1
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 10.6
(3)
A 		1
(-2.7)
A 		0.6
(-2.9)
A 		0.5
(-3)
A 		2
(-2.1)
A 		1.6
(-2.3)
A 		10
B 1.9
(1.8)
A 		0.8
(-2.6)
A 		0.7
(-3)
A 		0.7
(-3)
A 		2.2
(3)
A 		2.1
(2.6)
A 		2
C 1.5
(-2)
A 		2.2
(-0.4)
A 		1.3
(-2.5)
A 		2.5
(0.4)
A 		1.1
(-3)
A 		3.6
(3)
A 		3
avr 4.7
(3) 		1.3
(-2.4) 		0.9
(-3) 		1.2
(-2.5) 		1.8
(-1.6) 		2.4
(-0.6) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177573_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3454
Sequence Type Exemplar sequence
Representative Public ID E01G4.4 NCBI
Target Description E01G4.4 /REP_DB=WormBase Gene ID /WP=CE09111 /TR=O02238 /GB=CAB05716.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064432 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-27) (pqn-27) mRNA, complete cds. 11/11 None
E01G4.4 ENSEMBL cdna:known chromosome:CEL140:II:13458757:13460538:-1 gene:E01G4.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016566 3/11 Cross Hyb Matching Probes None
GENEFINDER00000016576 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13458753-13460535(-) 89.16 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pqn-27
Ensembl E01G4.4
Entrez Gene 3564944 Entrez gene
SwissProt O02238 EMBL-EBI
WormBase CE09111 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05716 Hypothetical protein E01G4.4 [Caenorhabditis elegans] ref|NP_496833.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-27) [Caenorhabditis elegans] 1.0E-107
Sequence
Target Sequence 
>C. ELEGANS:177573_AT
acgtatttgtgccacggggtcatgcacaacagcctccggcagaatacgggttttacaatg
ggccgcccgttcatcagcaacactatcagcagcagcagcagcatggagagaattccgagg
aaaagcgaaaaaatgcggtttttccggagaaaaccgaagaaaaacagctgcagaaacggc
ttttaactcggatttttcgtgaaaaagcacacattttgttgatatttatgcagcaacagc
atgtaaggacgattttctggcggaaaattcgagaaaaatggtcctttataccgatttcag
atattttttgcgaaaaaatgggcctaaaagagcaattttctgccttccgacggcgttttt
cgacgaaaatagtttttaacgtggattttccctggaaaatcggccctactggcacaaaaa
gcgccattttctccggttttgacagatatccggctttttttgaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTATTTGTGCCACGGGGTCATGC 343 91 350 Antisense
AACAGCCTCCGGCAGAATACGGGTT 644 121 377 Antisense
TTTACAATGGGCCGCCCGTTCATCA 353 665 401 Antisense
GCTGCAGAAACGGCTTTTAACTCGG 86 295 516 Antisense
TGTAAGGACGATTTTCTGGCGGAAA 67 559 591 Antisense
AAATGGTCCTTTATACCGATTTCAG 241 115 625 Antisense
AAAGAGCAATTTTCTGCCTTCCGAC 44 123 676 Antisense
GAAAATCGGCCCTACTGGCACAAAA 425 353 744 Antisense
GGCACAAAAAGCGCCATTTTCTCCG 140 527 760 Antisense
ATTTTCTCCGGTTTTGACAGATATC 294 15 775 Antisense
GACAGATATCCGGCTTTTTTTGAAG 466 365 790 Antisense
Sequence Source Affymetrix Proprietary Database