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[177570_s_at]

Description F21H7.3 /REP_DB=WormBase Gene ID /WP=CE19801 /TR=Q9XTV9 /GB=CAB07592.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 02      6grp_total > 36      4grp_total > 08      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 37.8
(-3)
A 		45.6
(-2.2)
A 		61.5
(-0.5)
P 		94.3
(3)
A 		57
B 70.7
(2.1)
P 		30
(-3)
A 		59.9
(0.8)
A 		77.5
(3)
A 		48
C 9.9
(-3)
A 		43.1
(-0.2)
A 		34.2
(-0.9)
A 		80.2
(3)
A 		70
avr 39.5
(-3) 		39.6
(-3) 		51.9
(-1.3) 		84
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 68.4
(-0.8)
A 		73.2
(-0.3)
M 		108.9
(3)
P 		85.2
(0.8)
P 		44.6
(-3)
M 		58.3
(-1.7)
P 		64
B 116.5
(3)
P 		76.9
(-0.6)
P 		73.9
(-0.8)
P 		57.2
(-2.3)
P 		49.7
(-3)
A 		77.2
(-0.5)
P 		67
C 117.6
(2.8)
P 		120.2
(3)
P 		49.8
(-3)
A 		50.9
(-2.9)
A 		54.8
(-2.6)
P 		100.8
(1.3)
P 		70
avr 100.8
(3) 		90.1
(1.7) 		77.5
(0.3) 		64.4
(-1.3) 		49.7
(-3) 		78.8
(0.4) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 13.8
(0.8)
A 		9.9
(-3)
A 		16.1
(3)
P 		13.8
(0.8)
A 		6
B 22.6
(3)
P 		7.4
(-3)
A 		13.9
(-0.4)
A 		16.5
(0.6)
A 		15
C 3.3
(-3)
A 		12.4
(2.4)
A 		11.9
(2.1)
A 		13.5
(3)
A 		10
avr 13.2
(1.2) 		9.9
(-3) 		14
(2.2) 		14.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 12.6
(-3)
A 		26
(3)
M 		23.7
(2)
P 		21.6
(1)
P 		13.5
(-2.6)
M 		14.2
(-2.3)
P 		13
B 40.5
(3)
P 		25.6
(-0.5)
P 		17.9
(-2.4)
P 		15.2
(-3)
P 		20.2
(-1.8)
A 		28.8
(0.2)
P 		25
C 43.6
(3)
P 		36.7
(1.8)
P 		12.7
(-2.6)
A 		10.2
(-3)
A 		17.7
(-1.7)
P 		33.7
(1.2)
P 		33
avr 32.2
(3) 		29.4
(2) 		18.1
(-2.1) 		15.7
(-3) 		17.1
(-2.5) 		25.6
(0.6) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177570_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13307
Sequence Type Exemplar sequence
Representative Public ID F21H7.3 NCBI
Target Description F21H7.3 /REP_DB=WormBase Gene ID /WP=CE19801 /TR=Q9XTV9 /GB=CAB07592.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074695(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074695 NCBI Caenorhabditis elegans F21H7.3 (F21H7.3) mRNA, complete cds. 11/11 None
GENEFINDER00000014732 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16233671:16235838:-1 11/11 None
F21H7.3 ENSEMBL cdna:known chromosome:CEL140:V:16235252:16235838:-1 gene:F21H7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16235277-16235838(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21H7.3
Entrez Gene 259671 Entrez gene
SwissProt Q9XTV9 EMBL-EBI
WormBase CE19801 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07592 Hypothetical protein F21H7.3 [Caenorhabditis elegans] ref|NP_507096.1| F21H7.3 [Caenorhabditis elegans] 1.0E-97
blast CAB07592 Hypothetical protein F21H7.3 [Caenorhabditis elegans] ref|NP_507096.1| F21H7.3 [Caenorhabditis elegans] 9.0E-95
blast CAB07589 Hypothetical protein F21H7.1 [Caenorhabditis elegans] ref|NP_507095.1| Glutathione S-Transferase family member (gst-22) [Caenorhabditis elegans] 3.0E-90
blast AAO38568 Glutathione s-transferase protein 34 [Caenorhabditis elegans] ref|NP_741060.2| Glutathione S-Transferase family member (gst-34) [Caenorhabditis elegans] 3.0E-70
blast CAE61131 Hypothetical protein CBG04891 [Caenorhabditis briggsae] 2.0E-24
blast CAD54132 Hypothetical protein F21H7.12 [Caenorhabditis elegans] ref|NP_872186.1| F21H7.12 [Caenorhabditis elegans] 1.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 6.5E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507096.1 4 5-27,37-56,85-107,127-149
F21H7.3 4 5-27,37-56,85-107,127-149
GENEFINDER00000014732 4 5-27,37-56,85-107,127-149
Sequence
Target Sequence 
>C. ELEGANS:177570_S_AT
gacacaccatatttgttacttggtaattttgaacttattcttccttattattccaatatg
gttcctatcaatcggctttaaaaaccaaggaaaatgtgagaacccgcttcttccattatg
gcttatttttgttggtattttaattattattgatagaattatttattggaaaattctatt
caataaagtgaagaaactcatggaggatgttccgcaagatggaagaaagaaaaactggtt
cgaaaatttttggtggatatcaataaaatatgcgttggaaggagtcatgttaatagcggt
tgcccttggagctatttggagctatgaacaactcggccgaccgatttatttatgcaaatc
ttctgttcttttctcggtttccggattttgtattattgcttccataacttatatagtctc
ct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACACCATATTTGTTACTTGGTA 568 363 15 Antisense
GAACTTATTCTTCCTTATTATTCCA 108 351 45 Antisense
ATATGGTTCCTATCAATCGGCTTTA 71 19 70 Antisense
AAAATGTGAGAACCCGCTTCTTCCA 322 133 105 Antisense
CCCGCTTCTTCCATTATGGCTTATT 484 261 117 Antisense
GAAACTCATGGAGGATGTTCCGCAA 52 357 207 Antisense
GGTTGCCCTTGGAGCTATTTGGAGC 697 505 312 Antisense
GAACAACTCGGCCGACCGATTTATT 231 353 340 Antisense
TATGCAAATCTTCTGTTCTTTTCTC 376 657 365 Antisense
GGTTTCCGGATTTTGTATTATTGCT 139 505 390 Antisense
GCTTCCATAACTTATATAGTCTCCT 354 305 412 Antisense
Sequence Source Affymetrix Proprietary Database