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[177556_at]

Description F35H8.2 /REP_DB=WormBase Gene ID /WP=CE00980 /TR=Q20081 /GB=CAA85324.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 72      6grp_total > 74      4grp_total > 02      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1883
1 2 3 4 range
A 285.9
(-2.7)
P 		232.6
(-2.9)
P 		205.5
(-3)
P 		2050
(3)
P 		1845
B 260.6
(-2.6)
P 		277.8
(-2.5)
P 		167.1
(-3)
P 		1641.7
(3)
P 		1475
C 223.9
(-3)
P 		230.6
(-3)
P 		326.2
(-2.5)
P 		1582
(3)
P 		1358
avr 256.8
(-2.9) 		247
(-2.9) 		232.9
(-3) 		1757.9
(3) 		1525

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 714
1 2 3 4 5 6 range
A 305
(-1.5)
P 		202.9
(-3)
P 		499.9
(1.3)
P 		621.7
(3)
P 		594.6
(2.6)
P 		428.1
(0.2)
P 		419
B 162.6
(-3)
P 		362.2
(-0.4)
P 		419.5
(0.4)
P 		622.1
(3)
P 		560.2
(2.2)
P 		488.3
(1.3)
P 		460
C 188.1
(-3)
P 		249.6
(-2.5)
P 		362.4
(-1.5)
P 		876.6
(3)
P 		424.8
(-0.9)
P 		490.7
(-0.4)
P 		689
avr 218.6
(-3) 		271.6
(-2.3) 		427.3
(-0.4) 		706.8
(3) 		526.5
(0.8) 		469
(0.1) 		488
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 104.1
(-1.7)
P 		50.5
(-3)
P 		53.7
(-2.9)
P 		300.1
(3)
P 		250
B 83.1
(-2.1)
P 		68.6
(-2.4)
P 		38.7
(-3)
P 		349.9
(3)
P 		311
C 74.1
(-2.8)
P 		66.3
(-3)
P 		113.7
(-1.6)
P 		266.8
(3)
P 		201
avr 87.1
(-2.4) 		61.8
(-3) 		68.7
(-2.8) 		305.6
(3) 		244

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 56.2
(-3)
P 		72.1
(-2.2)
P 		108.9
(-0.4)
P 		157.7
(1.9)
P 		179.4
(3)
P 		104.2
(-0.7)
P 		123
B 56.6
(-3)
P 		120.6
(-0.8)
P 		101.9
(-1.4)
P 		164.8
(0.8)
P 		227.6
(3)
P 		182.1
(1.4)
P 		171
C 69.7
(-3)
P 		76.2
(-2.6)
P 		92.2
(-1.7)
P 		176.3
(3)
P 		137.4
(0.8)
P 		164.3
(2.3)
P 		107
avr 60.8
(-3) 		89.6
(-1.6) 		101
(-1) 		166.3
(2.2) 		181.5
(3) 		150.2
(1.4) 		121
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177556_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3673
Sequence Type Exemplar sequence
Representative Public ID F35H8.2 NCBI
Target Description F35H8.2 /REP_DB=WormBase Gene ID /WP=CE00980 /TR=Q20081 /GB=CAA85324.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063653(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063653 NCBI Caenorhabditis elegans F35H8.2 (F35H8.2) mRNA, complete cds. 11/11 None
SNAP00000035508 ENSEMBL cdna:SNAP chromosome:CEL140:II:9555426:9557303:1 11/11 None
GENEFINDER00000035515 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9555426:9557303:1 11/11 None
F35H8.2 ENSEMBL cdna:known chromosome:CEL140:II:9555426:9557403:1 gene:F35H8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9555422-9557300(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H8.2
Entrez Gene 185325 Entrez gene
SwissProt Q20081 EMBL-EBI
WormBase CE00980 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8375 acetylglucosaminyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85324 Hypothetical protein F35H8.2 [Caenorhabditis elegans] ref|NP_496054.1| F35H8.2 [Caenorhabditis elegans] 0.0
blast CAE57902 Hypothetical protein CBG00952 [Caenorhabditis briggsae] 0.0
blast CAB04881 Hypothetical protein T27F6.1 [Caenorhabditis elegans] ref|NP_493165.2| T27F6.1 [Caenorhabditis elegans] 2.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR003406 EMBL-EBI Glycosyl transferase, family 14 1.7E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496054.1 1 12-34
F35H8.2 1 12-34
SNAP00000035508 1 12-34
GENEFINDER00000035515 1 12-34
Sequence
Target Sequence 
>C. ELEGANS:177556_AT
acagctcacggaactctgaatgctcatagaaggtgtctacgggaaattgatgaatttgac
tggaagcatgcagttattacagctgagcatgacatcccacttcacagtactaaattctta
tcgagaaggtctagacgattaagagatatggttgagatgaatggaattcatttcaaagaa
gaaacgttagcaaacgcaaatccaaacgatacttctagtcaattattccaagtgtctcga
aactggcttcaacatctctgctcagtggcaacaatttcccactctcaacacaaagtttta
tacgattatcttctgaaaactccagatttcgatttaccaaatgatcagcatcgagaaatt
tcggttgttgaacagaaatgtgatacgatgaaatatgatgttgatggatcttgtctgtac
gcgatggaggatttttctgcgattagaaacactacccgtctcttcattcgcgccgatccc
cactacgactttggtttcgtacaatgtgttcatgaagtggtctttgaacgaaccttcttc
gacccaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGCTCACGGAACTCTGAATGCTC 442 109 511 Antisense
TACAGCTGAGCATGACATCCCACTT 24 645 588 Antisense
GACATCCCACTTCACAGTACTAAAT 479 369 601 Antisense
TAGTCAATTATTCCAAGTGTCTCGA 223 653 726 Antisense
GTGTCTCGAAACTGGCTTCAACATC 552 483 742 Antisense
TTCAACATCTCTGCTCAGTGGCAAC 99 687 758 Antisense
AATTTCCCACTCTCAACACAAAGTT 211 175 783 Antisense
GTTGATGGATCTTGTCTGTACGCGA 233 439 910 Antisense
GCGATGGAGGATTTTTCTGCGATTA 138 291 931 Antisense
CGATTAGAAACACTACCCGTCTCTT 404 247 950 Antisense
TTGAACGAACCTTCTTCGACCCAAT 42 701 1034 Antisense
Sequence Source Affymetrix Proprietary Database