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[177553_at]

Description R155.4 /REP_DB=WormBase Gene ID /WP=CE12868 /TR=O01922 /GB=AAB54270.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 53      6grp_cgc > 62      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 48.2
(-0.7)
A 		91.4
(3)
A 		21.5
(-3)
A 		24.5
(-2.7)
A 		70
B 88.2
(3)
A 		69.3
(1.5)
A 		20.1
(-2.3)
A 		11
(-3)
A 		77
C 78.9
(3)
A 		20.1
(-3)
A 		75.7
(2.7)
A 		31.3
(-1.9)
A 		59
avr 71.8
(3) 		60.3
(1.6) 		39.1
(-1) 		22.3
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 25.9
(-0.1)
A 		34
(1.2)
A 		45.4
(3)
A 		7.4
(-3)
A 		38.4
(1.9)
A 		8.2
(-2.9)
A 		38
B 37.7
(0.5)
A 		55.7
(3)
A 		25.5
(-1.2)
A 		46.5
(1.7)
A 		14.2
(-2.8)
A 		12.8
(-3)
A 		43
C 39.1
(-0.9)
A 		93.7
(3)
A 		68.8
(1.2)
A 		46
(-0.4)
A 		15.3
(-2.7)
A 		10.7
(-3)
A 		83
avr 34.2
(-0.2) 		61.1
(3) 		46.6
(1.3) 		33.3
(-0.3) 		22.6
(-1.6) 		10.6
(-3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 17.5
(2.1)
A 		19.8
(3)
A 		5.6
(-2.3)
A 		3.6
(-3)
A 		16
B 28.1
(3)
A 		17.1
(0.4)
A 		4.7
(-2.4)
A 		2.3
(-3)
A 		26
C 26.1
(2.9)
A 		5.8
(-2.9)
A 		26.4
(3)
A 		5.3
(-3)
A 		21
avr 23.9
(3) 		14.2
(0.1) 		12.2
(-0.5) 		3.7
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 4.8
(-1.3)
A 		12.1
(3)
A 		9.9
(1.7)
A 		1.9
(-3)
A 		11.6
(2.7)
A 		2
(-2.9)
A 		10
B 13.1
(0.6)
A 		18.5
(3)
A 		6.2
(-2.4)
A 		12.3
(0.3)
A 		5.8
(-2.6)
A 		4.8
(-3)
A 		14
C 14.5
(-0.4)
A 		28.6
(3)
A 		17.5
(0.3)
A 		9.2
(-1.7)
A 		4.9
(-2.7)
A 		3.6
(-3)
A 		25
avr 10.8
(-0.3) 		19.7
(3) 		11.2
(-0.1) 		7.8
(-1.4) 		7.4
(-1.6) 		3.5
(-3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177553_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8137
Sequence Type Exemplar sequence
Representative Public ID R155.4 NCBI
Target Description R155.4 /REP_DB=WormBase Gene ID /WP=CE12868 /TR=O01922 /GB=AAB54270.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065085
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000032921 ENSEMBL cdna:SNAP chromosome:CEL140:III:1977303:1980450:1 None
NM_065085 NCBI Caenorhabditis elegans R155.4 (R155.4) mRNA, complete cds. None
GENEFINDER00000032925 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1977303:1980418:1 None
R155.4 ENSEMBL cdna:known chromosome:CEL140:III:1977303:1980450:1 gene:R155.4 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1977302-1980901(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R155.4
Entrez Gene 187910 Entrez gene
SwissProt O01922 EMBL-EBI
WormBase CE30297 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54270 Hypothetical protein R155.4 [Caenorhabditis elegans] ref|NP_497486.2| R155.4 [Caenorhabditis elegans] 0.0
blast AAD34647 Hypothetical protein H04J21.1 [Caenorhabditis elegans] ref|NP_497543.1| H04J21.1 [Caenorhabditis elegans] 1.0E-118
blast AAD34647 Hypothetical protein H04J21.1 [Caenorhabditis elegans] ref|NP_497543.1| H04J21.1 [Caenorhabditis elegans] 1.0E-115
blast AAK29876 Hypothetical protein Y22D7AR.12 [Caenorhabditis elegans] ref|NP_497451.1| Y22D7AR.12 [Caenorhabditis elegans] 1.0E-107
blast AAK29876 Hypothetical protein Y22D7AR.12 [Caenorhabditis elegans] ref|NP_497451.1| Y22D7AR.12 [Caenorhabditis elegans] 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 3.2E-27
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497486.2 1 798-820
R155.4 1 798-820
SNAP00000032921 1 799-821
GENEFINDER00000032925 1 798-820
Sequence
Target Sequence 
>C. ELEGANS:177553_AT
atggaagctgcaaactccaactcccttgccggtttgctgccgattctaaataacttggag
agtattggattgaacttttcctcaagttcattctccaaatcgaaacaatcacttaaagtg
ctagacacctcgtttgccagtctcaacaatacaattaacaatagtcggcttgcatcaaaa
accaatgcacaagaagagcctggggctttggcaaaacataccatctacatcgttgccatc
ggaattccacttctcatcatcattgctgtgtgtgtcggtgtgtggacttggtggcgcaga
aggagtcagagaaggagagcagccgaggcttttgctcataggaatgacccgactccagag
tccagaagaagccagtcgaaaaatcaactgagaccgggtctgctgttcttgctcctccgg
aaaaatccaaataattttcagaatgaaatgaaaagaaacatatattttatttatggtttt
ccgtatcaagtaataaccctagaagcccaactgccactcgtacttcctcgtggcatcgat
ttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGAAGCTGCAAACTCCAACTCCC 320 53 2179 Antisense
TAAAGTGCTAGACACCTCGTTTGCC 244 641 2292 Antisense
CTCGTTTGCCAGTCTCAACAATACA 167 233 2307 Antisense
TACCATCTACATCGTTGCCATCGGA 394 651 2397 Antisense
GTGTGTGGACTTGGTGGCGCAGAAG 517 485 2456 Antisense
GAGAGCAGCCGAGGCTTTTGCTCAT 141 395 2493 Antisense
ATAGGAATGACCCGACTCCAGAGTC 43 21 2516 Antisense
ACTGAGACCGGGTCTGCTGTTCTTG 72 99 2565 Antisense
CTGTTCTTGCTCCTCCGGAAAAATC 364 235 2581 Antisense
GTAATAACCCTAGAAGCCCAACTGC 117 421 2668 Antisense
GTACTTCCTCGTGGCATCGATTTAC 150 461 2698 Antisense
Sequence Source Affymetrix Proprietary Database