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[177548_at]

Description W02B8.3 /REP_DB=WormBase Gene ID /WP=CE20132 /TR=Q9XVH3 /GB=CAB03459.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 53      4grp_total > 51      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 67
(-3)
A 		127.3
(3)
A 		118.1
(2.1)
A 		70.3
(-2.7)
A 		60
B 48.7
(-0.6)
A 		112.8
(3)
A 		105.7
(2.6)
A 		5.2
(-3)
A 		108
C 132
(1.7)
A 		149.9
(3)
A 		75.8
(-2.5)
A 		68.8
(-3)
A 		81
avr 82.6
(-0.5) 		130
(3) 		99.9
(0.8) 		48.1
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 90.8
(2.3)
A 		32.1
(-2.8)
A 		98.4
(3)
A 		38
(-2.3)
A 		69.9
(0.5)
A 		30.3
(-3)
A 		68
B 3.2
(-3)
A 		47.4
(-0.4)
A 		72.6
(1.1)
A 		104.5
(3)
A 		87.3
(2)
A 		46.1
(-0.5)
A 		101
C 91.4
(2.8)
A 		49.5
(-0.6)
A 		93.4
(3)
A 		20.4
(-3)
A 		77.5
(1.7)
A 		59.7
(0.2)
A 		73
avr 61.8
(-0.5) 		43
(-3) 		88.1
(3) 		54.3
(-1.5) 		78.2
(1.7) 		45.4
(-2.7) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 24.4
(1.1)
A 		27.6
(2)
A 		30.9
(3)
A 		10.3
(-3)
A 		21
B 15.5
(0.2)
A 		27.9
(3)
A 		24.5
(2.2)
A 		1.1
(-3)
A 		27
C 43.7
(3)
A 		43.1
(2.9)
A 		26.4
(-0.2)
A 		11.6
(-3)
A 		32
avr 27.9
(1.8) 		32.9
(3) 		27.3
(1.7) 		7.7
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 16.7
(1)
A 		11.4
(-1.3)
A 		21.4
(3)
A 		9.6
(-2.1)
A 		21.1
(2.9)
A 		7.4
(-3)
A 		14
B 1.1
(-3)
A 		15.8
(-0.4)
A 		17.6
(-0.1)
A 		27.7
(1.6)
A 		35.5
(3)
A 		17.2
(-0.2)
A 		34
C 33.9
(3)
A 		15.1
(-0.8)
A 		23.8
(1)
A 		4.1
(-3)
A 		25.1
(1.2)
A 		20
(0.2)
A 		30
avr 17.2
(-1.5) 		14.1
(-2.9) 		20.9
(0.2) 		13.8
(-3) 		27.2
(3) 		14.9
(-2.5) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177548_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2921
Sequence Type Exemplar sequence
Representative Public ID W02B8.3 NCBI
Target Description W02B8.3 /REP_DB=WormBase Gene ID /WP=CE20132 /TR=Q9XVH3 /GB=CAB03459.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064514(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064514 NCBI Caenorhabditis elegans W02B8.3 (W02B8.3) mRNA, complete cds. 11/11 None
SNAP00000004893 ENSEMBL cdna:SNAP chromosome:CEL140:II:13916247:13919569:1 11/11 None
GENEFINDER00000004899 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13916247:13919569:1 11/11 None
W02B8.3 ENSEMBL cdna:known chromosome:CEL140:II:13916247:13919569:1 gene:W02B8.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064515 1/11 Cross Hyb Matching Probes None
GENEFINDER00000004900 1/11 Cross Hyb Matching Probes None
W02B8.4 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13916243-13919566(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02B8.3
Entrez Gene 189108 Entrez gene
SwissProt Q9XVH3 EMBL-EBI
WormBase CE20132 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03459 Hypothetical protein W02B8.3 [Caenorhabditis elegans] ref|NP_496915.1| W02B8.3 [Caenorhabditis elegans] 0.0
blast CAE73323 Hypothetical protein CBG20752 [Caenorhabditis briggsae] 0.0
blast CAI46577 Hypothetical protein W02B8.6 [Caenorhabditis elegans] ref|NP_001022392.1| W02B8.6 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496915.1 3 595-617,630-652,680-702
W02B8.3 3 595-617,630-652,680-702
SNAP00000004893 3 595-617,630-652,680-702
GENEFINDER00000004899 3 595-617,630-652,680-702
Sequence
Target Sequence 
>C. ELEGANS:177548_AT
gaagttgaaattcgatgtccgccaaaaagacatcgtcctctccccaatcgtcaacacacc
aatcatcaacaaccctgccttagcatcccaggcactcatcctctccccagccctcttcgt
ccccctgattcaatctcccgccgtattcggaaccgtggtgctctctccatggctcttcgt
ccccgtaattctgtcgcctcgtgtgctgagcccggtaatcctcgacccgttcatgttcgt
cccaatcgtcctatccccattggcgctggtcccggtagtcctgtcgccaggggtattcaa
tccattcatcttgtccccactagtcctgtgccccttcgttctgtcccctcaagtcatgac
cccactgattttgtcgccattcgcactgacccccttgatcctgaacccgctactcatgtc
tccagtaatactgtcaccattcgtgctatccccacaagtgttgtcacctcagtttttgac
cggaatcgttttatccccgtctgcactgtcaccagctattcagtcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTGAAATTCGATGTCCGCCAA 447 337 1671 Antisense
CCGCCAAAAAGACATCGTCCTCTCC 492 261 1689 Antisense
AATCGTCAACACACCAATCATCAAC 513 165 1716 Antisense
GTATTCGGAACCGTGGTGCTCTCTC 345 453 1813 Antisense
GTAGTCCTGTCGCCAGGGGTATTCA 683 433 1945 Antisense
TATTCAATCCATTCATCTTGTCCCC 512 663 1964 Antisense
CAAGTCATGACCCCACTGATTTTGT 494 181 2020 Antisense
CCGCTACTCATGTCTCCAGTAATAC 455 263 2077 Antisense
AATACTGTCACCATTCGTGCTATCC 180 173 2097 Antisense
GTTGTCACCTCAGTTTTTGACCGGA 399 435 2130 Antisense
GCACTGTCACCAGCTATTCAGTCGG 64 315 2173 Antisense
Sequence Source Affymetrix Proprietary Database