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[177547_at]

Description ZK858.7 /REP_DB=WormBase Gene ID /WP=CE15433 /TR=Q94416 /GB=CAB02135.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 94      6grp_total > 76      4grp_total > 62      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 611.6
(2.8)
P 		584.5
(0.3)
P 		613.8
(3)
P 		548.8
(-3)
P 		65
B 677.1
(3)
P 		597.8
(-0.1)
P 		616.6
(0.7)
P 		521.6
(-3)
P 		156
C 719.5
(3)
P 		533.4
(-1)
P 		619.3
(0.9)
P 		438.3
(-3)
P 		281
avr 669.4
(3) 		571.9
(-0.5) 		616.6
(1.1) 		502.9
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 606
1 2 3 4 5 6 range
A 358.8
(-3)
P 		397.9
(-2.4)
P 		512.2
(-0.7)
P 		455.3
(-1.5)
P 		555.5
(-0)
P 		754.5
(3)
P 		396
B 535.8
(-1.6)
P 		443.5
(-3)
P 		636.4
(-0)
P 		705.5
(1)
P 		621.4
(-0.3)
P 		832.5
(3)
P 		389
C 411.2
(-3)
P 		566.2
(-1.3)
P 		429.9
(-2.8)
P 		546.6
(-1.5)
P 		489.4
(-2.2)
P 		964.7
(3)
P 		554
avr 435.3
(-3) 		469.2
(-2.5) 		526.2
(-1.7) 		569.1
(-1.1) 		555.4
(-1.3) 		850.6
(3) 		415
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 222.7
(3)
P 		126.9
(-1)
P 		160.5
(0.4)
P 		80.3
(-3)
P 		142
B 215.9
(3)
P 		147.6
(-0.9)
P 		142.9
(-1.2)
P 		111.2
(-3)
P 		105
C 238.2
(3)
P 		153.4
(-0.1)
P 		215.9
(2.2)
P 		73.9
(-3)
P 		164
avr 225.6
(3) 		142.6
(-0.6) 		173.1
(0.7) 		88.5
(-3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 66.1
(-3)
P 		141.5
(0.8)
P 		111.5
(-0.7)
P 		115.5
(-0.5)
P 		167.6
(2.2)
P 		183.7
(3)
P 		118
B 186.4
(-1.6)
P 		147.6
(-3)
P 		154.5
(-2.7)
P 		186.9
(-1.6)
P 		252.5
(0.9)
P 		310.4
(3)
P 		163
C 152.4
(-1.8)
P 		172.8
(-1.2)
P 		109.4
(-3)
P 		109.9
(-3)
P 		158.4
(-1.6)
P 		322.9
(3)
P 		214
avr 135
(-2.6) 		154
(-1.8) 		125.1
(-3) 		137.4
(-2.5) 		192.8
(-0.2) 		272.3
(3) 		147
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177547_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2294
Sequence Type Exemplar sequence
Representative Public ID ZK858.7 NCBI
Target Description ZK858.7 /REP_DB=WormBase Gene ID /WP=CE15433 /TR=Q94416 /GB=CAB02135.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060051(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060051 NCBI Caenorhabditis elegans ZK858.7 (ZK858.7) mRNA, complete cds. 11/11 None
GENEFINDER00000011882 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9150110:9152493:-1 11/11 None
SNAP00000011879 ENSEMBL cdna:SNAP chromosome:CEL140:I:9150204:9152493:-1 11/11 None
ZK858.7 ENSEMBL cdna:known chromosome:CEL140:I:9150110:9152493:-1 gene:ZK858.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9150040-9152424(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK858.7
Entrez Gene 259389 Entrez gene
SwissProt Q94416 EMBL-EBI
WormBase CE35204 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13634_S_AT eukaryotic initiation factor 3 gamma subunit family protein at
ATH1-121501:266924_AT eukaryotic initiation factor 3 gamma subunit family protein at
CANINE_2:CFA.2816.1.A1_AT similar to CGI-09 protein cfa
CANINE_2:CFAAFFX.9933.1.S1_S_AT similar to CGI-09 protein cfa
DROSOPHILA_2:1636478_AT dm
DROSGENOME1:145910_AT dm
CHICKEN:GGAAFFX.12535.1.S1_AT similar to CGI-09 protein gga
CHICKEN:GGAAFFX.5795.1.S1_AT similar to CGI-09 protein gga
HG-U133_PLUS_2:233970_S_AT CGI-09 protein hs
HG-U133B:233970_S_AT CGI-09 protein hs
HU35KSUBC:RC_AA464166_AT CGI-09 protein hs
HU35KSUBD:RC_AA405657_AT CGI-09 protein hs
HU35KSUBD:RC_AA284153_AT CGI-09 protein hs
U133_X3P:HS.128791.1.S1_3P_A_AT CGI-09 protein hs
U133_X3P:HS.128791.0.S1_3P_A_AT CGI-09 protein hs
HG-U133_PLUS_2:222768_S_AT CGI-09 protein hs
HG-U133B:222768_S_AT CGI-09 protein hs
HG-U95B:49026_AT CGI-09 protein hs
HG-U95B:59737_AT CGI-09 protein hs
HG-U95C:59945_AT CGI-09 protein hs
MOUSE430_2:1438326_AT RIKEN cDNA 3300001M20 gene mm
MOE430B:1438326_AT RIKEN cDNA 3300001M20 gene mm
MG-U74BV2:111372_AT RIKEN cDNA 3300001M20 gene mm
MG-U74CV2:168841_F_AT RIKEN cDNA 3300001M20 gene mm
MOE430B:1431212_A_AT RIKEN cDNA 3300001M20 gene mm
MOUSE430_2:1431212_A_AT RIKEN cDNA 3300001M20 gene mm
MOE430B:1428754_AT RIKEN cDNA 3300001M20 gene mm
MOUSE430_2:1428754_AT RIKEN cDNA 3300001M20 gene mm
MG-U74CV2:167257_I_AT MKIAA1153 protein mm
MU19KSUBA:TC18208_AT MKIAA1153 protein mm
MU19KSUBB:TC30809_AT MKIAA1153 protein mm
RG-U34A:RC_AI639158_AT similar to CGI-09 protein (predicted) rn
RG-U34C:RC_AI137337_AT similar to CGI-09 protein (predicted) rn
RAE230A:1381217_AT similar to CGI-09 protein (predicted) rn
RAT230_2:1381217_AT similar to CGI-09 protein (predicted) rn
RAE230B:1377625_AT similar to CGI-09 protein (predicted) rn
RAT230_2:1377625_AT similar to CGI-09 protein (predicted) rn
RAE230A:1377021_AT Similar to CGI-09 protein (predicted) rn
RAT230_2:1377021_AT Similar to CGI-09 protein (predicted) rn
RG-U34C:AA944061_AT Similar to CGI-09 protein (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6446 regulation of translational initiation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02135 Hypothetical protein ZK858.7 [Caenorhabditis elegans] ref|NP_492452.2| ZK858.7 [Caenorhabditis elegans] 0.0
blast CAE67047 Hypothetical protein CBG12454 [Caenorhabditis briggsae] 0.0
blast XP_640865 hypothetical protein DDB0204062 [Dictyostelium discoideum] gb|EAL66884.1| hypothetical protein DDB0204062 [Dictyostelium discoideum] 2.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR007316 EMBL-EBI Eukaryotic initiation factor 3, gamma subunit 1.0E-126
Pfam IPR007316 EMBL-EBI Eukaryotic initiation factor 3, gamma subunit 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492452.2 1 426-448
ZK858.7 1 426-448
GENEFINDER00000011882 2 177-196,438-460
Sequence
Target Sequence 
>C. ELEGANS:177547_AT
gggtgcggtagctcaatcaattccctgtgttcattctatgaattacgatgataacacact
ttccacatttttaccggttcgaattaagtgtgttctagcaggcaaacatctgccatatga
caataaccgaaaggctgtaaatggggatgatgataatttggaggaagtcaatggagataa
ggaacctgagaaaatcgaggatcaagatctagaggctcttgctcgccgaaatgatcgttt
ggatcgggagaagagaggaatcgatttgattaatgatgaacaaattcattctttaattat
tggaagtcgtactgtcgatcctatcagtgttttggagcttctttatcccaaactggcacc
atctgggacgattgtcatctactctcctcatcaaaatatcctcatctcagcatacgattg
gttgtcaaagcgtcaaacaatcaatttagttcttactgatcaaatgtgtcgtgtaatgca
agttctacctgatagaactcatccgttaatggctcagcacgtcgctggcggacatattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTGCGGTAGCTCAATCAATTCCC 501 495 828 Antisense
ATCAATTCCCTGTGTTCATTCTATG 671 25 843 Antisense
GATGATAACACACTTTCCACATTTT 196 411 874 Antisense
GAGGCTCTTGCTCGCCGAAATGATC 506 405 1039 Antisense
GAAGTCGTACTGTCGATCCTATCAG 30 337 1130 Antisense
TGGAGCTTCTTTATCCCAAACTGGC 412 545 1160 Antisense
ACTGGCACCATCTGGGACGATTGTC 648 95 1179 Antisense
TCTCCTCATCAAAATATCCTCATCT 217 611 1210 Antisense
ATCCTCATCTCAGCATACGATTGGT 625 39 1225 Antisense
GAACTCATCCGTTAATGGCTCAGCA 709 349 1322 Antisense
AGCACGTCGCTGGCGGACATATTCT 224 77 1343 Antisense
Sequence Source Affymetrix Proprietary Database