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[177546_at]

Description C54G4.7 /REP_DB=WormBase Gene ID /WP=CE05513 /TR=Q18852 /GB=CAA99819.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 30      4grp_high_8_8 > 07      6grp_highest_nonorm > 74      6grp_total > 75      4grp_total > 01      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 836
1 2 3 4 range
A 469.4
(-1.3)
P 		275.6
(-3)
P 		437.8
(-1.6)
P 		950.2
(3)
P 		675
B 421.7
(-2.8)
P 		398.6
(-3)
P 		419
(-2.8)
P 		1111.2
(3)
P 		713
C 469.6
(-1.8)
P 		353.5
(-3)
P 		483.7
(-1.7)
P 		949.7
(3)
P 		596
avr 453.6
(-2) 		342.6
(-3) 		446.8
(-2.1) 		1003.7
(3) 		661

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 452.1
(-3)
P 		578.2
(-0.9)
P 		633.1
(0)
P 		808.9
(3)
P 		598.6
(-0.5)
P 		674.9
(0.7)
P 		357
B 586
(-3)
P 		641.3
(-1.1)
P 		659.4
(-0.4)
P 		592.6
(-2.8)
P 		589.4
(-2.9)
P 		757
(3)
P 		171
C 522.1
(-3)
P 		631.3
(-1.1)
P 		684.1
(-0.1)
P 		859.3
(3)
P 		566.9
(-2.2)
P 		848
(2.8)
P 		337
avr 520.1
(-3) 		616.9
(-0.6) 		658.9
(0.5) 		753.6
(2.8) 		585
(-1.4) 		760
(3) 		240
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 170.9
(3)
P 		59.8
(-3)
P 		114.4
(-0.1)
P 		139.1
(1.3)
P 		111
B 134.5
(-1.4)
P 		98.4
(-2.9)
P 		97.1
(-3)
P 		236.8
(3)
P 		140
C 155.5
(1.8)
P 		101.6
(-3)
P 		168.6
(3)
P 		160.1
(2.2)
P 		67
avr 153.6
(1.4) 		86.6
(-3) 		126.7
(-0.4) 		178.7
(3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 83.3
(-3)
P 		205.6
(3)
P 		137.9
(-0.3)
P 		205.2
(3)
P 		180.6
(1.8)
P 		164.3
(1)
P 		122
B 203.9
(-0.8)
P 		213.5
(-0.3)
P 		160.1
(-2.9)
P 		157
(-3)
P 		239.5
(1)
P 		282.3
(3)
P 		125
C 193.5
(-1.9)
P 		192.6
(-1.9)
P 		174.1
(-2.9)
P 		172.8
(-3)
P 		183.4
(-2.4)
P 		283.9
(3)
P 		111
avr 160.2
(-2.8) 		203.9
(0.2) 		157.4
(-3) 		178.3
(-1.5) 		201.2
(0.1) 		243.5
(3) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177546_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.443
Sequence Type Exemplar sequence
Representative Public ID C54G4.7 NCBI
Target Description C54G4.7 /REP_DB=WormBase Gene ID /WP=CE05513 /TR=Q18852 /GB=CAA99819.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059805(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059805 NCBI Caenorhabditis elegans C54G4.7 (C54G4.7) mRNA, complete cds. 11/11 None
SNAP00000028901 ENSEMBL cdna:SNAP chromosome:CEL140:I:8029171:8030603:1 11/11 None
GENEFINDER00000028906 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8029171:8030603:1 11/11 None
C54G4.7.1 ENSEMBL cdna:known chromosome:CEL140:I:8027627:8030841:1 gene:C54G4.7 11/11 None
C54G4.7.4 ENSEMBL cdna:known chromosome:CEL140:I:8027838:8030915:1 gene:C54G4.7 11/11 None
C54G4.7.2 ENSEMBL cdna:known chromosome:CEL140:I:8027838:8030841:1 gene:C54G4.7 11/11 None
C54G4.7.3 ENSEMBL cdna:known chromosome:CEL140:I:8027841:8030841:1 gene:C54G4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8028910-8030534(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54G4.7
Entrez Gene 3565589 Entrez gene
SwissProt Q18852 EMBL-EBI
WormBase CE05513 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6715.1.S1_AT similar to CG32112-PC, isoform C cfa
CANINE_2:CFAAFFX.6715.1.S1_S_AT similar to CG32112-PC, isoform C cfa
DROSOPHILA_2:1628887_A_AT dm
CHICKEN:GGAAFFX.21430.1.S1_AT similar to CG32112-PB gga
CHICKEN:GGAAFFX.6418.2.S1_S_AT similar to CG32112-PB gga
HG-U133_PLUS_2:219980_AT hypothetical protein FLJ21106 hs
HG-U133A:219980_AT hypothetical protein FLJ21106 hs
HG-U133A_2:219980_AT hypothetical protein FLJ21106 hs
HU35KSUBD:RC_H29460_AT hypothetical protein FLJ21106 hs
U133_X3P:G13376659_3P_AT hypothetical protein FLJ21106 hs
U133_X3P:HS.164302.0.A1_3P_AT hypothetical protein FLJ21106 hs
HG-U133_PLUS_2:236240_AT hypothetical protein FLJ21106 hs
HG-U133B:236240_AT hypothetical protein FLJ21106 hs
HG-U95D:84690_AT hypothetical protein FLJ21106 hs
HG-U95B:48131_R_AT Hypothetical protein FLJ21106 hs
HG-U95B:51424_AT Hypothetical protein FLJ21106 hs
HG-U95C:63288_AT Hypothetical protein FLJ21106 hs
HU35KSUBB:RC_R12689_AT Hypothetical protein FLJ21106 hs
HU35KSUBB:RC_T98858_AT Hypothetical protein FLJ21106 hs
HU35KSUBD:RC_AA403151_AT Hypothetical protein FLJ21106 hs
MOUSE430_2:1432485_AT RIKEN cDNA 3110057O12 gene mm
MOE430B:1432485_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430_2:1455475_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430A_2:1455475_AT RIKEN cDNA 3110057O12 gene mm
MOE430A:1455475_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430_2:1452477_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430A_2:1452477_AT RIKEN cDNA 3110057O12 gene mm
MOE430A:1452477_AT RIKEN cDNA 3110057O12 gene mm
MG-U74BV2:115521_AT RIKEN cDNA 3110057O12 gene mm
MG-U74CV2:140861_AT RIKEN cDNA 3110057O12 gene mm
MG-U74CV2:133667_AT RIKEN cDNA 3110057O12 gene mm
MG-U74CV2:167106_AT RIKEN cDNA 3110057O12 gene mm
MOE430B:1429966_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430_2:1429966_AT RIKEN cDNA 3110057O12 gene mm
MOE430B:1438283_AT RIKEN cDNA 3110057O12 gene mm
MOUSE430_2:1438283_AT RIKEN cDNA 3110057O12 gene mm
RG-U34C:RC_AI237398_AT hypothetical protein LOC499602 rn
RAE230B:1390830_AT hypothetical protein LOC499602 rn
RAT230_2:1390830_AT hypothetical protein LOC499602 rn
Protein Similarities
Method ID Description E-Value
blast CAA99819 Hypothetical protein C54G4.7 [Caenorhabditis elegans] ref|NP_492206.1| C54G4.7 [Caenorhabditis elegans] 0.0
blast CAE63696 Hypothetical protein CBG08211 [Caenorhabditis briggsae] 1.0E-177
blast CAE63696 Hypothetical protein CBG08211 [Caenorhabditis briggsae] 1.0E-176
Sequence
Target Sequence 
>C. ELEGANS:177546_AT
gacggatctttttgtgatgggtgccgctttaattgcagaatgtaatcatcttttcaattg
gtctgagacacttggatatggtccatttgcaatttctggtgtctcgatgggtggatttat
ggctcaacttgctggatcaaattcgcaacgtccaatatcaatagttcctattcttgcatg
gacgacagcatcaccatcatatacggaaggagctatttctccagctgttaattattcttt
actgcagaaacagctcgaagatcctcactatgttgataaaatccgaagaattccgaatca
aaattggctggataaaatgcgagagatgactgcgagaaacggtgattcagaagccaagaa
tatgatgagaattttgatggatgacttcacaagtcttgaattctatccaacaccaataga
cacatcactatgccatgtattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGGATCTTTTTGTGATGGGTGCC 170 377 522 Antisense
GATGGGTGCCGCTTTAATTGCAGAA 511 407 537 Antisense
GGATATGGTCCATTTGCAATTTCTG 596 507 595 Antisense
GCAATTTCTGGTGTCTCGATGGGTG 444 323 610 Antisense
GATTTATGGCTCAACTTGCTGGATC 392 429 635 Antisense
GATCAAATTCGCAACGTCCAATATC 545 421 656 Antisense
TAGTTCCTATTCTTGCATGGACGAC 511 653 683 Antisense
ACGACAGCATCACCATCATATACGG 437 91 703 Antisense
GAAGGAGCTATTTCTCCAGCTGTTA 169 335 727 Antisense
GAAACAGCTCGAAGATCCTCACTAT 693 355 768 Antisense
GACACATCACTATGCCATGTATTCT 228 367 940 Antisense
Sequence Source Affymetrix Proprietary Database