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[177539_at]

Description ZK858.2 /REP_DB=WormBase Gene ID /WP=CE15423 /TR=Q94418 /GB=CAB02137.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.8
(-3)
A 		3.2
(-2)
A 		2.2
(-2.7)
A 		9.8
(3)
A 		8
B 1.8
(-3)
A 		2.3
(3)
A 		1.9
(-1.8)
A 		2.2
(1.8)
A 		0
C 1.4
(-2.9)
A 		2.1
(-2.2)
A 		1.3
(-3)
A 		7.5
(3)
A 		6
avr 1.7
(-3) 		2.5
(-2) 		1.8
(-2.9) 		6.5
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 224
1 2 3 4 5 6 range
A 3.5
(-2.9)
A 		1.4
(-3)
A 		3.3
(-2.9)
A 		9
(-2.7)
A 		107.1
(1.2)
P 		153.1
(3)
P 		152
B 1.5
(-3)
A 		1.4
(-3)
A 		6.2
(-2.9)
A 		1.5
(-3)
A 		225.3
(3)
P 		204.4
(2.4)
P 		224
C 3.4
(-2.9)
A 		1.7
(-3)
A 		2.5
(-3)
A 		2.8
(-3)
A 		91.2
(-0.3)
P 		202.8
(3)
P 		201
avr 2.8
(-3) 		1.5
(-3) 		4
(-2.9) 		4.4
(-2.9) 		141.2
(1.5) 		186.8
(3) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 0.7
(-2.3)
A 		0.7
(-2.3)
A 		0.6
(-3)
A 		1.4
(3)
A 		1
B 0.6
(3)
A 		0.6
(3)
A 		0.4
(-3)
A 		0.5
(0)
A 		0
C 0.5
(-3)
A 		0.6
(-2.3)
A 		0.5
(-3)
A 		1.3
(3)
A 		1
avr 0.6
(-2) 		0.6
(-2) 		0.5
(-3) 		1.1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 0.6
(-3)
A 		0.5
(-3)
A 		0.7
(-3)
A 		2.3
(-2.7)
A 		32.3
(2.2)
P 		37.3
(3)
P 		37
B 0.5
(-3)
A 		0.5
(-3)
A 		1.5
(-2.9)
A 		0.4
(-3)
A 		91.6
(3)
P 		76.2
(2)
P 		91
C 1.3
(-2.9)
A 		0.5
(-3)
A 		0.6
(-3)
A 		0.6
(-3)
A 		29.5
(-0.4)
P 		67.9
(3)
P 		67
avr 0.8
(-3) 		0.5
(-3) 		0.9
(-3) 		1.1
(-2.9) 		51.1
(2.1) 		60.5
(3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1633
Sequence Type Exemplar sequence
Representative Public ID ZK858.2 NCBI
Target Description ZK858.2 /REP_DB=WormBase Gene ID /WP=CE15423 /TR=Q94418 /GB=CAB02137.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060046(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060046 NCBI Caenorhabditis elegans ZK858.2 (ZK858.2) mRNA, complete cds. 11/11 None
SNAP00000011873 ENSEMBL cdna:SNAP chromosome:CEL140:I:9131324:9132565:-1 11/11 None
GENEFINDER00000011885 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9131324:9132565:-1 11/11 None
ZK858.2 ENSEMBL cdna:known chromosome:CEL140:I:9131324:9132565:-1 gene:ZK858.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9131254-9132496(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK858.2
Entrez Gene 191443 Entrez gene
SwissProt Q94418 EMBL-EBI
WormBase CE15423 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02137 Hypothetical protein ZK858.2 [Caenorhabditis elegans] ref|NP_492447.1| ZK858.2 [Caenorhabditis elegans] 1.0E-108
blast CAE67041 Hypothetical protein CBG12448 [Caenorhabditis briggsae] 7.0E-32
Sequence
Target Sequence 
>C. ELEGANS:177539_AT
gtttctccaacagatttcatgccaatcagagaacttgtcacaactccatatcacatttcg
actccagtgttaataactccagttgatgcaagatcgatctcaagagaaattgtacgtgtc
aatcgtataagacgtgttcgaactcgtcaagcatctcgttccgtattcaatagtgttcgt
cgtcgactttcaatgataacagctaatcgaactgaaattcaaatgaatactccacaaaca
ttgccacgtagcaaacgaatcgacaacacttcgacagccatggaaccagtgagttcgaat
gcttttcagaaaattatttctgaaattctgaataactcacctgcgtgtacagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTCTCCAACAGATTTCATGCCAA 342 451 154 Antisense
TCAGAGAACTTGTCACAACTCCATA 456 623 179 Antisense
CAACTCCATATCACATTTCGACTCC 214 189 194 Antisense
TTCGACTCCAGTGTTAATAACTCCA 192 695 210 Antisense
GTGTTCGAACTCGTCAAGCATCTCG 447 485 287 Antisense
GTATTCAATAGTGTTCGTCGTCGAC 248 453 316 Antisense
TTCGTCGTCGACTTTCAATGATAAC 286 695 329 Antisense
GAATACTCCACAAACATTGCCACGT 379 327 378 Antisense
GACAACACTTCGACAGCCATGGAAC 79 365 415 Antisense
ACCAGTGAGTTCGAATGCTTTTCAG 470 85 438 Antisense
GAATAACTCACCTGCGTGTACAGAA 552 325 483 Antisense
Sequence Source Affymetrix Proprietary Database