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[177533_at]

Description F35E2.3 /REP_DB=WormBase Gene ID /WP=CE18662 /TR=O62229 /GB=CAB04289.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 81      4grp_total > 51      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 31
(2.2)
A 		33.4
(3)
A 		32.5
(2.7)
A 		15
(-3)
A 		18
B 40.8
(3)
A 		40.7
(3)
A 		34.1
(1.6)
A 		11.4
(-3)
A 		29
C 3.2
(-3)
A 		62.3
(3)
A 		16.3
(-1.7)
A 		9.4
(-2.4)
A 		59
avr 25
(-0.7) 		45.5
(3) 		27.6
(-0.2) 		11.9
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 34.1
(0.8)
A 		8.9
(-2.3)
A 		52.5
(3)
A 		45.6
(2.2)
A 		39
(1.4)
A 		3.4
(-3)
A 		49
B 21.7
(-1.1)
A 		13.6
(-2.9)
A 		24.8
(-0.4)
A 		30.9
(1)
A 		39.9
(3)
A 		13
(-3)
A 		27
C 11.3
(-2.8)
A 		43.1
(2.8)
A 		10.9
(-2.8)
A 		44.1
(3)
A 		35
(1.4)
A 		9.9
(-3)
A 		34
avr 22.4
(-0.4) 		21.9
(-0.5) 		29.4
(0.9) 		40.2
(3) 		38
(2.6) 		8.8
(-3) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 11.3
(3)
A 		7.3
(0.4)
A 		8.5
(1.2)
A 		2.2
(-3)
A 		9
B 13
(3)
A 		10.1
(1.4)
A 		7.9
(0.1)
A 		2.4
(-3)
A 		11
C 1.1
(-3)
A 		17.9
(3)
A 		5.7
(-1.4)
A 		1.6
(-2.8)
A 		17
avr 8.5
(1) 		11.8
(3) 		7.4
(0.3) 		2.1
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 6.3
(0)
A 		3.2
(-1.7)
A 		11.4
(2.8)
A 		11.6
(2.9)
A 		11.8
(3)
A 		0.8
(-3)
A 		11
B 7.5
(-1.5)
A 		4.5
(-3)
A 		6
(-2.2)
A 		8.2
(-1.1)
A 		16.2
(3)
A 		4.8
(-2.8)
A 		12
C 4.2
(-2.2)
A 		13.2
(3)
A 		2.8
(-3)
A 		8.9
(0.5)
A 		11.3
(1.9)
A 		3.3
(-2.7)
A 		10
avr 6
(-1.2) 		7
(-0.6) 		6.7
(-0.8) 		9.6
(0.9) 		13.1
(3) 		3
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177533_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.525
Sequence Type Exemplar sequence
Representative Public ID F35E2.3 NCBI
Target Description F35E2.3 /REP_DB=WormBase Gene ID /WP=CE18662 /TR=O62229 /GB=CAB04289.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060588 NCBI Caenorhabditis elegans F35E2.3 (F35E2.3) mRNA, complete cds. 11/11 None
SNAP00000041136 ENSEMBL cdna:SNAP chromosome:CEL140:I:11742550:11744086:-1 11/11 None
F35E2.3 ENSEMBL cdna:known chromosome:CEL140:I:11742550:11744058:-1 gene:F35E2.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041149 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11742480-11744017(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E2.3
Entrez Gene 185286 Entrez gene
SwissProt O62229 EMBL-EBI
WormBase CE34303 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04289 Hypothetical protein F35E2.3 [Caenorhabditis elegans] ref|NP_492989.2| F35E2.3 [Caenorhabditis elegans] 8.0E-76
blast CAB04289 Hypothetical protein F35E2.3 [Caenorhabditis elegans] ref|NP_492989.2| F35E2.3 [Caenorhabditis elegans] 2.0E-70
blast CAB04288 Hypothetical protein F35E2.9 [Caenorhabditis elegans] ref|NP_492987.2| F35E2.9 [Caenorhabditis elegans] 2.0E-13
blast CAB04309 Hypothetical protein F36D1.6 [Caenorhabditis elegans] ref|NP_492894.2| F36D1.6 [Caenorhabditis elegans] 3.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 1.8E-37
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 8.0E-23
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 1.8E-37
Sequence
Target Sequence 
>C. ELEGANS:177533_AT
ttctggaagctctctgccgaggagaacgatgaacgtctagcgtcatgtggacaggagcat
cttccccgccgcttcacagacggagtatcgttcaaagttgacccagccaaatggacgctt
actctggaaaatagtatggcaaactcaaaaattgtcacaactgcgagcatgatttctact
cgtcattttttgagctcttcccgagcagttatggaaggtgacacgtggatttacgatgaa
ggaaatacacatcaacgtccccggcaacgctctttccacgttgagaattattggaaaagt
ctgtccacacccgtcctactgcccgaaaatgtcatttatacttgcaaggcagtgattctg
aatggatgtctggaatcttcaaatacgacatattcccatccaatgatcattcaaatcaat
caggattcgccggttttcaccagttatccatgcttggacgatggcagcacccggctccga
acctctgatcaggtcgacgcgtttggtttcaatttcaatggagatttgatgcatagaaca
gttttcctgaaacatcgtacttattcccactactacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTGGAAGCTCTCTGCCGAGGAGA 395 695 73 Antisense
TCATGTGGACAGGAGCATCTTCCCC 275 623 115 Antisense
CCGCCGCTTCACAGACGGAGTATCG 679 261 138 Antisense
GTATCGTTCAAAGTTGACCCAGCCA 311 455 157 Antisense
GACCCAGCCAAATGGACGCTTACTC 114 381 172 Antisense
TACTCGTCATTTTTTGAGCTCTTCC 220 647 249 Antisense
TCAATCAGGATTCGCCGGTTTTCAC 339 631 488 Antisense
GTTTTCACCAGTTATCCATGCTTGG 694 447 505 Antisense
GAACCTCTGATCAGGTCGACGCGTT 143 347 551 Antisense
GTCGACGCGTTTGGTTTCAATTTCA 243 473 565 Antisense
ATCGTACTTATTCCCACTACTACGT 621 33 626 Antisense
Sequence Source Affymetrix Proprietary Database