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[177532_at]

Description F22B5.4 /REP_DB=WormBase Gene ID /WP=CE02199 /TR=Q19708 /GB=CAA90356.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 35      6grp_total > 73      4grp_total > 52      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2611
1 2 3 4 range
A 2793.7
(2)
P 		3217.8
(3)
P 		1768.3
(-0.5)
P 		758.2
(-3)
P 		2460
B 2901.7
(2.5)
P 		3110.4
(3)
P 		1677.6
(-0.5)
P 		665.1
(-3)
P 		2445
C 3155.8
(3)
P 		3025
(2.7)
P 		1832.8
(-0.1)
P 		607.3
(-3)
P 		2549
avr 2950.4
(2.6) 		3117.7
(3) 		1759.6
(-0.3) 		676.9
(-3) 		2441

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6729
1 2 3 4 5 6 range
A 949.8
(-3)
P 		1874.8
(-2.1)
P 		4347.3
(0.4)
P 		6877.1
(3)
P 		3436.4
(-0.5)
P 		2682.2
(-1.2)
P 		5927
B 881.5
(-3)
P 		1913.9
(-1.6)
P 		4080.7
(1.2)
P 		5415.5
(3)
P 		3774.3
(0.8)
P 		2579.9
(-0.8)
P 		4534
C 1043.3
(-3)
P 		1624.9
(-2.5)
P 		4905.9
(0.5)
P 		7610.9
(3)
P 		3358.4
(-0.9)
P 		2624.2
(-1.6)
P 		6568
avr 958.2
(-3) 		1804.5
(-2.1) 		4444.6
(0.7) 		6634.5
(3) 		3523
(-0.3) 		2628.8
(-1.2) 		5676
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 942
1 2 3 4 range
A 1017.2
(3)
P 		698.5
(0.9)
P 		462.3
(-0.7)
P 		111
(-3)
P 		906
B 925.3
(3)
P 		768.2
(1.8)
P 		388.7
(-1.1)
P 		141.7
(-3)
P 		784
C 1044.6
(3)
P 		869.7
(1.9)
P 		638.8
(0.4)
P 		102.4
(-3)
P 		942
avr 995.7
(3) 		778.8
(1.5) 		496.6
(-0.4) 		118.4
(-3) 		877

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1569
1 2 3 4 5 6 range
A 175.1
(-3)
P 		666.8
(-1.1)
P 		946.8
(-0)
P 		1744.2
(3)
P 		1037
(0.3)
P 		653
(-1.2)
P 		1569
B 306.7
(-3)
P 		637
(-1.4)
P 		990.9
(0.3)
P 		1434.4
(2.5)
P 		1533.4
(3)
P 		962
(0.2)
P 		1227
C 386.7
(-3)
P 		495.8
(-2.4)
P 		1248.6
(1.5)
P 		1530.6
(3)
P 		1086.7
(0.7)
P 		878.5
(-0.4)
P 		1144
avr 289.5
(-3) 		599.9
(-1.5) 		1062.1
(0.6) 		1569.7
(3) 		1219
(1.4) 		831.2
(-0.5) 		1280
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177532_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3194
Sequence Type Exemplar sequence
Representative Public ID F22B5.4 NCBI
Target Description F22B5.4 /REP_DB=WormBase Gene ID /WP=CE02199 /TR=Q19708 /GB=CAA90356.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063380(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063380 NCBI Caenorhabditis elegans F22B5.4 (F22B5.4) mRNA, complete cds. 11/11 None
SNAP00000010404 ENSEMBL cdna:SNAP chromosome:CEL140:II:8452050:8452593:1 11/11 None
GENEFINDER00000010410 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8452050:8452593:1 11/11 None
F22B5.4 ENSEMBL cdna:known chromosome:CEL140:II:8452049:8452601:1 gene:F22B5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8452046-8452590(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22B5.4
Entrez Gene 174348 Entrez gene
SwissProt Q19708 EMBL-EBI
WormBase CE02199 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90356 Hypothetical protein F22B5.4 [Caenorhabditis elegans] ref|NP_495781.1| F22B5.4 [Caenorhabditis elegans] 1.0E-85
blast CAE57640 Hypothetical protein CBG00625 [Caenorhabditis briggsae] 2.0E-68
blast CAB03069 Hypothetical protein F36A2.7 [Caenorhabditis elegans] ref|NP_492383.1| F36A2.7 [Caenorhabditis elegans] 3.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495781.1 1 79-101
F22B5.4 1 79-101
SNAP00000010404 1 79-101
GENEFINDER00000010410 1 79-101
Sequence
Target Sequence 
>C. ELEGANS:177532_AT
tgtcgttgagcaaagccccacgtaagacaatgtttcgtttcgtgttgtcacgaaatgcca
gcacatcaaatgttccatcgccagcaagaattcaactcaaaaagccagctgaggcgggac
atttccaatattctcgtaattggtcaagagatccacgttttgttaaagtcgccattcaga
agggagatactccttatcaattccttgtccgccgtctcggtcacgcttatgaagtatatc
cactctttgtgctcactgcagcctggttcgttctcttctgcagtgcatcatactggagtt
ttggaaaagccgaaatctggcttgacagaagcaattccaaagcaccatgggactgggaga
gacttcgtgacacgtactggaagatgccaacagttgcatttgacctcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCGTTGAGCAAAGCCCCACGTAA 446 469 14 Antisense
GCCAGCACATCAAATGTTCCATCGC 350 279 70 Antisense
GTTCCATCGCCAGCAAGAATTCAAC 520 441 85 Antisense
GGCGGGACATTTCCAATATTCTCGT 216 541 126 Antisense
GATCCACGTTTTGTTAAAGTCGCCA 364 415 163 Antisense
AGATACTCCTTATCAATTCCTTGTC 692 63 198 Antisense
CCGTCTCGGTCACGCTTATGAAGTA 27 265 225 Antisense
TGAAGTATATCCACTCTTTGTGCTC 27 575 243 Antisense
TCGTTCTCTTCTGCAGTGCATCATA 84 605 281 Antisense
GGGACTGGGAGAGACTTCGTGACAC 436 495 362 Antisense
GCCAACAGTTGCATTTGACCTCGAT 471 277 399 Antisense
Sequence Source Affymetrix Proprietary Database