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[177524_at]

Description K03D7.6 /REP_DB=WormBase Gene ID /WP=CE16220 /TR=O45651 /GB=CAB04557.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 64      4grp_total > 28      6grp_cgc > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 23.4
(2.9)
A 		18.9
(1.6)
A 		2.6
(-3)
A 		23.9
(3)
A 		21
B 4.5
(-1.4)
A 		11.3
(3)
A 		6.7
(-0)
A 		2.1
(-3)
A 		9
C 3.7
(-2.3)
A 		2.8
(-3)
A 		3.5
(-2.5)
A 		10.7
(3)
A 		8
avr 10.5
(1.7) 		11
(2.1) 		4.3
(-3) 		12.2
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 5.5
(-0.8)
A 		12.9
(3)
A 		1.2
(-3)
A 		6.2
(-0.4)
A 		2.6
(-2.3)
A 		3.4
(-1.9)
A 		12
B 2.9
(-1.6)
A 		2.6
(-1.9)
A 		1.8
(-2.7)
A 		1.5
(-3)
A 		1.6
(-2.9)
A 		7.6
(3)
A 		6
C 3.2
(-3)
A 		3
(-3)
A 		36.3
(3)
A 		8.2
(-2.1)
A 		18.2
(-0.3)
A 		24.6
(0.9)
A 		33
avr 3.9
(-3) 		6.2
(-1.5) 		13.1
(3) 		5.3
(-2.1) 		7.5
(-0.7) 		11.9
(2.2) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 8.5
(3)
A 		4.1
(-0.4)
A 		0.7
(-3)
A 		3.5
(-0.8)
A 		8
B 1.4
(-0.5)
A 		2.8
(3)
A 		1.6
(0)
A 		0.4
(-3)
A 		2
C 1.2
(-0.6)
A 		0.8
(-3)
A 		1.2
(-0.6)
A 		1.8
(3)
A 		1
avr 3.7
(3) 		2.6
(0.4) 		1.2
(-3) 		1.9
(-1.3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1
(-2)
A 		4.6
(3)
A 		0.3
(-3)
A 		1.6
(-1.2)
A 		0.8
(-2.3)
A 		0.8
(-2.3)
A 		4
B 1
(-1.5)
A 		0.9
(-1.8)
A 		0.4
(-3)
A 		0.4
(-3)
A 		0.6
(-2.5)
A 		2.8
(3)
A 		2
C 1.2
(-2.8)
A 		0.9
(-3)
A 		9.2
(3)
A 		1.6
(-2.5)
A 		5.9
(0.6)
A 		8.2
(2.3)
A 		8
avr 1.1
(-3) 		2.1
(-0.9) 		3.3
(1.7) 		1.2
(-2.8) 		2.4
(-0.2) 		3.9
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177524_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13339
Sequence Type Exemplar sequence
Representative Public ID K03D7.6 NCBI
Target Description K03D7.6 /REP_DB=WormBase Gene ID /WP=CE16220 /TR=O45651 /GB=CAB04557.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075032 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-118) (srh-118) mRNA, complete cds. 11/11 None
SNAP00000027356 ENSEMBL cdna:SNAP chromosome:CEL140:V:17496882:17498474:-1 11/11 None
K03D7.6 ENSEMBL cdna:known chromosome:CEL140:V:17496882:17498747:-1 gene:K03D7.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000027370 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17274306-17275465(+) 91.5 91.5
chrV:17496881-17498747(-) 99.81 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-118
Ensembl K03D7.6
Entrez Gene 186945 Entrez gene
SwissProt O45651 EMBL-EBI
WormBase CE16220 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04557 Hypothetical protein K03D7.6 [Caenorhabditis elegans] ref|NP_507433.1| Serpentine Receptor, class H family member (srh-118) [Caenorhabditis elegans] 0.0
blast T23586 hypothetical protein K10G4.6 - Caenorhabditis elegans 1.0E-177
blast CAB04557 Hypothetical protein K03D7.6 [Caenorhabditis elegans] ref|NP_507433.1| Serpentine Receptor, class H family member (srh-118) [Caenorhabditis elegans] 1.0E-147
blast CAA16414 Hypothetical protein Y61B8A.1 [Caenorhabditis elegans] ref|NP_507371.2| Serpentine Receptor, class H family member (srh-116) [Caenorhabditis elegans] 1.0E-139
blast T23586 hypothetical protein K10G4.6 - Caenorhabditis elegans 1.0E-124
blast CAA16414 Hypothetical protein Y61B8A.1 [Caenorhabditis elegans] ref|NP_507371.2| Serpentine Receptor, class H family member (srh-116) [Caenorhabditis elegans] 3.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.8E-104
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507433.1 7 31-53,66-88,108-130,151-168,220-242,255-277,292-314
K03D7.6 7 31-53,66-88,108-130,151-168,220-242,255-277,292-314
SNAP00000027356 5 20-39,60-77,129-151,164-186,201-223
Sequence
Target Sequence 
>C. ELEGANS:177524_AT
aataccatgcttattggcagtttttgtgggaaaggttataccagaagatcaggatgctgc
aaagttatttgcattaatagaatatccatgtccaacaagagagtttttcacatctgaagt
attgattctctttgctgacaagactataatagaaggcgttgttcgatttttcccatttgg
ttgtgcctacatctcaagcttcttcctttttcaagttgcaactctgatctactacatttg
tattgcaccttcaaacacgacttcaagagatacccagcaaaaacagaaagtgtttctcat
ttgcattttacttcaaacttctattcctctgtttcttgggatgtgtccaatcatgttgac
tttcatggccttcttaaccggtcactattatcaggaaatggcgaatcttactttttgtag
tcttggacttcacggtatggcagagagcattgctattgtcactgtgcatcgacctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACCATGCTTATTGGCAGTTTTT 49 173 486 Antisense
GAAGTATTGATTCTCTTTGCTGACA 168 337 601 Antisense
AAGCTTCTTCCTTTTTCAAGTTGCA 102 139 681 Antisense
TCAAGTTGCAACTCTGATCTACTAC 418 631 696 Antisense
TTACTTCAAACTTCTATTCCTCTGT 52 683 793 Antisense
ATTCCTCTGTTTCTTGGGATGTGTC 307 7 808 Antisense
GGGATGTGTCCAATCATGTTGACTT 687 495 823 Antisense
CATGTTGACTTTCATGGCCTTCTTA 476 209 837 Antisense
GGAAATGGCGAATCTTACTTTTTGT 332 523 879 Antisense
CTTGGACTTCACGGTATGGCAGAGA 587 217 907 Antisense
GCTATTGTCACTGTGCATCGACCTT 601 303 937 Antisense
Sequence Source Affymetrix Proprietary Database