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[177521_at]

Description F58E6.6 /REP_DB=WormBase Gene ID /WP=CE06021 /TR=Q20982 /GB=CAA94776.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 28      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 26.6
(3)
A 		9.9
(-2.6)
A 		8.7
(-3)
A 		23.2
(1.9)
A 		18
B 14
(-2.2)
A 		42.4
(3)
A 		9.7
(-3)
A 		20.8
(-1)
A 		33
C 19.5
(-1.6)
A 		26.5
(-0.7)
A 		7.3
(-3)
A 		57.9
(3)
A 		51
avr 20
(-0.3) 		26.3
(1.2) 		8.6
(-3) 		34
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 5.1
(-2.2)
A 		4.3
(-2.5)
A 		12.9
(0.6)
A 		3
(-3)
A 		19.4
(3)
A 		3.8
(-2.7)
A 		16
B 11.6
(-2.3)
A 		8.2
(-2.7)
A 		62
(3)
A 		5.6
(-3)
A 		5.2
(-3)
A 		6.8
(-2.8)
A 		57
C 32.8
(3)
A 		5.7
(-2.8)
A 		4.8
(-3)
A 		8
(-2.3)
A 		6.4
(-2.7)
A 		7.3
(-2.5)
A 		28
avr 16.5
(0.1) 		6.1
(-2.8) 		26.6
(3) 		5.5
(-3) 		10.3
(-1.6) 		6
(-2.9) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 9.7
(3)
A 		2.2
(-3)
A 		2.3
(-2.9)
A 		3.4
(-2)
A 		8
B 4.5
(-1.3)
A 		10.5
(3)
A 		2.2
(-3)
A 		4.4
(-1.4)
A 		8
C 6.4
(0.2)
A 		7.6
(1.2)
A 		2.6
(-3)
A 		9.8
(3)
A 		7
avr 6.9
(3) 		6.8
(2.9) 		2.4
(-3) 		5.9
(1.7) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.9
(-2.9)
A 		1.5
(-2.2)
A 		2.8
(-0.6)
A 		0.8
(-3)
A 		5.8
(3)
A 		0.9
(-2.9)
A 		5
B 4
(-1.9)
A 		2.7
(-2.5)
A 		15.1
(3)
A 		1.5
(-3)
A 		2.1
(-2.7)
A 		2.6
(-2.5)
A 		14
C 12.2
(3)
A 		1.7
(-2.7)
A 		1.2
(-3)
A 		1.6
(-2.8)
A 		2.1
(-2.5)
A 		2.5
(-2.3)
A 		11
avr 5.7
(2.2) 		2
(-2.2) 		6.4
(3) 		1.3
(-3) 		3.3
(-0.6) 		2
(-2.2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177521_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11550
Sequence Type Exemplar sequence
Representative Public ID F58E6.6 NCBI
Target Description F58E6.6 /REP_DB=WormBase Gene ID /WP=CE06021 /TR=Q20982 /GB=CAA94776.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073114(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073114 NCBI Caenorhabditis elegans F58E6.6 (F58E6.6) mRNA, complete cds. 11/11 None
SNAP00000040367 ENSEMBL cdna:SNAP chromosome:CEL140:V:9754659:9755444:-1 11/11 None
GENEFINDER00000040377 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9754659:9757091:-1 11/11 None
F58E6.6 ENSEMBL cdna:known chromosome:CEL140:V:9754659:9755444:-1 gene:F58E6.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9754658-9755444(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58E6.6
Entrez Gene 186525 Entrez gene
SwissProt Q20982 EMBL-EBI
WormBase CE06021 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94775 Hypothetical protein F58E6.5 [Caenorhabditis elegans] ref|NP_505516.1| F58E6.5 [Caenorhabditis elegans] 1.0E-159
blast CAA94776 Hypothetical protein F58E6.6 [Caenorhabditis elegans] ref|NP_505515.1| F58E6.6 [Caenorhabditis elegans] 4.0E-85
blast CAA94776 Hypothetical protein F58E6.6 [Caenorhabditis elegans] ref|NP_505515.1| F58E6.6 [Caenorhabditis elegans] 1.0E-84
blast CAE64695 Hypothetical protein CBG09477 [Caenorhabditis briggsae] 1.0E-83
blast CAE63974 Hypothetical protein CBG08565 [Caenorhabditis briggsae] 9.0E-18
blast CAE62601 Hypothetical protein CBG06717 [Caenorhabditis briggsae] 3.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 2.5E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505515.1 2 7-29,168-190
F58E6.6 2 7-29,168-190
SNAP00000040367 2 7-29,168-190
GENEFINDER00000040377 1 473-495
Sequence
Target Sequence 
>C. ELEGANS:177521_AT
aaattgaccacaagttctctccattcggaacattttcatttggcaatgggaaaaccacgt
gttgtcagctttcattctcttgtcgaccacgaaacgcttcaagttattttctcactttat
ggaggagctccacaagagcacacttatgaaatgagaaagtctccagatgccggacaagac
ttctatgtatttggagaagttgttgttcttgtacagtcaagttttgaatgcgacagtgga
tttactggatcgagatgtcagagaatcaaagtcaaaggaactaagtcttcaactacaact
acatcatctgtttctccaacaactgtatccacttcaacctccattccgtcttcaagatca
ttcgctgtaatcagtgtctcgaactatgtcatccttcatatcattctcgcaatcgctcta
gcagttctatttattctcctcgtcgtcgttctctggatcgtctgccgtcgtcggactgtt
aggcttgactcggaaaccgtttgcgaatcggcggaaaacaatagtaatatgtcaactgaa
gatgattctggaatcggaagtgggttgagctataccacagccaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATTGACCACAAGTTCTCTCCATT 298 115 127 Antisense
AAAACCACGTGTTGTCAGCTTTCAT 457 131 177 Antisense
CATTCTCTTGTCGACCACGAAACGC 35 217 199 Antisense
GAAAGTCTCCAGATGCCGGACAAGA 158 361 281 Antisense
ACAACTACATCATCTGTTTCTCCAA 410 113 421 Antisense
TCCAACAACTGTATCCACTTCAACC 695 587 441 Antisense
CCGTCTTCAAGATCATTCGCTGTAA 89 265 472 Antisense
TGTCTCGAACTATGTCATCCTTCAT 184 559 501 Antisense
TCTCGCAATCGCTCTAGCAGTTCTA 492 611 531 Antisense
TGCCGTCGTCGGACTGTTAGGCTTG 379 579 589 Antisense
TGGGTTGAGCTATACCACAGCCACA 116 553 687 Antisense
Sequence Source Affymetrix Proprietary Database