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[177520_at]

Description T03D8.4 /REP_DB=WormBase Gene ID /WP=CE18922 /TR=Q9XTY2 /GB=CAB07409.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 01      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 13.5
(-2.9)
A 		13.1
(-3)
A 		37.8
(1.1)
A 		49
(3)
A 		36
B 50
(3)
A 		31.9
(0)
A 		13.4
(-3)
A 		23.5
(-1.3)
A 		37
C 25.9
(-3)
A 		35.1
(-1.5)
A 		39.9
(-0.8)
A 		63.9
(3)
A 		38
avr 29.8
(-2) 		26.7
(-3) 		30.4
(-1.8) 		45.5
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 112.7
(2.7)
A 		27.4
(-3)
A 		52.4
(-1.3)
A 		117.1
(3)
A 		101.5
(2)
A 		86.6
(1)
A 		90
B 46.8
(-1.8)
A 		46
(-1.8)
A 		29
(-3)
A 		117.5
(3)
A 		54.6
(-1.3)
A 		52.6
(-1.4)
A 		89
C 62.6
(-0.1)
A 		63.9
(-0.1)
A 		51.4
(-0.8)
A 		112.4
(3)
A 		74.9
(0.6)
A 		17.1
(-3)
A 		95
avr 74
(-0.5) 		45.8
(-2.9) 		44.3
(-3) 		115.7
(3) 		77
(-0.3) 		52.1
(-2.3) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 4.9
(-1.2)
A 		2.8
(-3)
A 		9.9
(3)
A 		7.2
(0.7)
A 		7
B 16
(3)
A 		7.9
(-0.8)
A 		3.1
(-3)
A 		5
(-2.1)
A 		13
C 8.6
(-3)
A 		10.1
(-1.3)
A 		13.9
(3)
A 		10.8
(-0.5)
A 		5
avr 9.8
(3) 		6.9
(-3) 		9
(1.3) 		7.7
(-1.3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 20.8
(0.2)
A 		9.7
(-3)
A 		11.4
(-2.5)
A 		29.7
(2.7)
A 		30.6
(3)
A 		21.1
(0.3)
A 		21
B 16.3
(-0.7)
A 		15.3
(-0.9)
A 		7
(-3)
A 		31.1
(3)
A 		22.2
(0.8)
A 		19.6
(0.1)
A 		24
C 23.2
(2.7)
A 		19.5
(1.5)
A 		13.1
(-0.6)
A 		22.6
(2.5)
A 		24.2
(3)
A 		5.7
(-3)
A 		19
avr 20.1
(0.3) 		14.8
(-1.5) 		10.5
(-3) 		27.8
(3) 		25.7
(2.3) 		15.5
(-1.3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177520_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13331
Sequence Type Exemplar sequence
Representative Public ID T03D8.4 NCBI
Target Description T03D8.4 /REP_DB=WormBase Gene ID /WP=CE18922 /TR=Q9XTY2 /GB=CAB07409.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075618(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075618 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-14) (grl-14) mRNA, complete cds. 11/11 None
SNAP00000040019 ENSEMBL cdna:SNAP chromosome:CEL140:V:20823927:20827039:1 11/11 None
GENEFINDER00000040029 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20823732:20825718:1 11/11 None
T03D8.4 ENSEMBL cdna:known chromosome:CEL140:V:20823732:20825718:1 gene:T03D8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20823731-20825718(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-14
Ensembl T03D8.4
Entrez Gene 188013 Entrez gene
SwissProt Q9XTY2 EMBL-EBI
WormBase CE18922 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07409 Hypothetical protein T03D8.4 [Caenorhabditis elegans] ref|NP_508019.1| GRound-Like (grd related) family member (grl-14) [Caenorhabditis elegans] 0.0
blast CAB07409 Hypothetical protein T03D8.4 [Caenorhabditis elegans] ref|NP_508019.1| GRound-Like (grd related) family member (grl-14) [Caenorhabditis elegans] 1.0E-170
blast CAE61636 Hypothetical protein CBG05569 [Caenorhabditis briggsae] 1.0E-146
blast CAE61636 Hypothetical protein CBG05569 [Caenorhabditis briggsae] 1.0E-144
blast CAE61636 Hypothetical protein CBG05569 [Caenorhabditis briggsae] 1.0E-110
blast CAE61260 Hypothetical protein CBG05066 [Caenorhabditis briggsae] 3.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.3E-28
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 1.9E-24
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.3E-28
Sequence
Target Sequence 
>C. ELEGANS:177520_AT
gagattcgatatgcctaccgtactacgaatcggcagcctgttgtctacgttccttctgct
tcacccgttaatcagccatcacatgcttcgaatactgtacccgtggctgtcgcttcatac
ctagtaactgcaaatccatcatcacaaccaattcaactccatattttgccaccaattcca
tctccccagccacaaataatctatcaacaaacgacaccatcaagccaatttgttgcttat
tcaacctttgcaccacctatagagccatcgaatgaatgtggaaatggtcagtgcaaacca
gaatcggatgaagataaatgcaacagccagcgattacgggacattatcttcaataatatt
gtttccggcgatgctgaagcttccaaaagagcagttcaaagtgcagctgaagctgaaact
ggtttattttttgatgcaatctgcggcactggtttcttcagctacattgcacacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATTCGATATGCCTACCGTACTA 528 395 682 Antisense
CGAATCGGCAGCCTGTTGTCTACGT 581 253 707 Antisense
ACATGCTTCGAATACTGTACCCGTG 11 105 762 Antisense
ATCATCACAACCAATTCAACTCCAT 118 31 819 Antisense
ACTCCATATTTTGCCACCAATTCCA 514 99 837 Antisense
ATCTCCCCAGCCACAAATAATCTAT 528 33 861 Antisense
TTGCTTATTCAACCTTTGCACCACC 23 705 914 Antisense
TTGCACCACCTATAGAGCCATCGAA 188 703 929 Antisense
TTTCCGGCGATGCTGAAGCTTCCAA 705 667 1043 Antisense
ATGCAATCTGCGGCACTGGTTTCTT 260 43 1115 Antisense
GTTTCTTCAGCTACATTGCACACAC 113 451 1133 Antisense
Sequence Source Affymetrix Proprietary Database