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[177519_at]

Description F35E2.9 /REP_DB=WormBase Gene ID /WP=CE17778 /TR=O62231 /GB=CAB04288.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 59      4grp_total > 25      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 2.1
(-3)
A 		30.6
(3)
A 		24.9
(1.8)
A 		27.2
(2.3)
A 		29
B 13.1
(-3)
A 		21.9
(-1.2)
A 		22.4
(-1.1)
A 		43
(3)
A 		30
C 4.5
(-3)
A 		23.6
(1.3)
A 		28
(2.3)
A 		31.3
(3)
A 		27
avr 6.6
(-3) 		25.4
(1.1) 		25.1
(1.1) 		33.8
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 45.6
(3)
A 		1.4
(-3)
A 		5.8
(-2.4)
A 		20.4
(-0.4)
A 		9.8
(-1.9)
A 		35.9
(1.7)
A 		44
B 2.4
(-3)
A 		16
(0.3)
A 		3.1
(-2.8)
A 		2.3
(-3)
A 		9.9
(-1.2)
A 		27.4
(3)
A 		25
C 5.4
(-2)
A 		13.9
(-0.2)
A 		1
(-3)
A 		6.5
(-1.8)
A 		28.4
(3)
A 		24.4
(2.1)
A 		27
avr 17.8
(0.4) 		10.4
(-1.4) 		3.3
(-3) 		9.7
(-1.5) 		16
(-0.1) 		29.2
(3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 0.8
(-3)
A 		6.6
(3)
A 		6.5
(2.9)
A 		4
(0.3)
A 		6
B 4.2
(-3)
A 		5.4
(-1.6)
A 		5.2
(-1.8)
A 		9.2
(3)
A 		5
C 1.5
(-3)
A 		6.8
(0.8)
A 		9.8
(3)
A 		5.3
(-0.3)
A 		8
avr 2.2
(-3) 		6.3
(1.9) 		7.2
(3) 		6.2
(1.8) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 8.4
(2.8)
A 		0.5
(-3)
A 		1.3
(-2.4)
A 		5.2
(0.4)
A 		3
(-1.2)
A 		8.7
(3)
A 		8
B 0.8
(-2.9)
A 		5.3
(-0.1)
A 		0.8
(-2.9)
A 		0.6
(-3)
A 		4
(-0.9)
A 		10.2
(3)
A 		10
C 2
(-1.9)
A 		4.2
(-0.4)
A 		0.3
(-3)
A 		1.3
(-2.3)
A 		9.2
(3)
A 		8.2
(2.3)
A 		9
avr 3.7
(-0.9) 		3.3
(-1.2) 		0.8
(-3) 		2.4
(-1.8) 		5.4
(0.4) 		9
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177519_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2527
Sequence Type Exemplar sequence
Representative Public ID F35E2.9 NCBI
Target Description F35E2.9 /REP_DB=WormBase Gene ID /WP=CE17778 /TR=O62231 /GB=CAB04288.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060586
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000041131 ENSEMBL cdna:SNAP chromosome:CEL140:I:11723078:11732367:-1 11/11 None
GENEFINDER00000041141 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11728063:11734384:-1 11/11 None
F35E2.9 ENSEMBL cdna:known chromosome:CEL140:I:11728024:11734404:-1 gene:F35E2.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11727954-11734315(-) 100.0 100.0
Public Domain and Genome References
Ensembl F35E2.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04285 Hypothetical protein F35E2.7 [Caenorhabditis elegans] ref|NP_492985.1| F35E2.7 [Caenorhabditis elegans] 0.0
blast CAB04287 Hypothetical protein F35E2.6 [Caenorhabditis elegans] ref|NP_492984.1| F35E2.6 [Caenorhabditis elegans] 0.0
blast CAB04108 Hypothetical protein F14B6.5 [Caenorhabditis elegans] ref|NP_493089.2| F14B6.5 [Caenorhabditis elegans] 0.0
blast CAB04288 Hypothetical protein F35E2.9 [Caenorhabditis elegans] ref|NP_492987.2| F35E2.9 [Caenorhabditis elegans] 0.0
blast CAA21047 Hypothetical protein Y47H9B.2 [Caenorhabditis elegans] ref|NP_492993.2| Y47H9B.2 [Caenorhabditis elegans] 2.0E-18
blast CAE67998 Hypothetical protein CBG13608 [Caenorhabditis briggsae] 2.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.0E-126
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 5.5E-44
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 2.0E-89
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 8.5E-83
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 3.4E-89
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 1.7E-79
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 2.0E-89
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 8.5E-83
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000041131 11 840-862,894-911,921-940,960-982,1020-1042,1054-1076,1135-1157,1164-1181,1191-1213,1220-1242,1247-1269
Sequence
Target Sequence 
>C. ELEGANS:177519_AT
acaaatccatgttactcattgcaacaattgaaaatggttatccagaaaatacaagtcatc
cttgcctagctgatgaaaataccattaatcagtctgatggcttattcgacctttacgaat
ctacaaaaaattggcatagacacatgagcttgatgagcacaaacacaagtgatgactcgt
tttatgttgatttttacattcattatggaatagtcaccaaaataatcaatgataaggaaa
cgctttttggaattggtctcatgtacgacggaggcgctaagaaaattacacactacagtg
atgtgatctgcaaactagctggaatttgctctcagttttctaatggcactcaatttccac
ataattcaaatgatccatcatccatatccaaagtcactacactgtctttcaattcaactc
agccaaccattaccaaaaacattactcccgtggatgccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAATCCATGTTACTCATTGCAAC 432 111 3182 Antisense
ACAAGTCATCCTTGCCTAGCTGATG 537 113 3232 Antisense
TCTGATGGCTTATTCGACCTTTACG 400 609 3274 Antisense
GAAACGCTTTTTGGAATTGGTCTCA 168 359 3418 Antisense
TGGTCTCATGTACGACGGAGGCGCT 440 551 3435 Antisense
AACTAGCTGGAATTTGCTCTCAGTT 682 139 3494 Antisense
GCTCTCAGTTTTCTAATGGCACTCA 476 299 3509 Antisense
ATCCAAAGTCACTACACTGTCTTTC 303 39 3567 Antisense
ACTGTCTTTCAATTCAACTCAGCCA 350 97 3582 Antisense
ACTCAGCCAACCATTACCAAAAACA 43 87 3598 Antisense
AAAACATTACTCCCGTGGATGCCAA 165 131 3617 Antisense
Sequence Source Affymetrix Proprietary Database