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[177478_at]

Description D1086.2 /REP_DB=WormBase Gene ID /WP=CE09060 /TR=O17727 /GB=CAB04020.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      6grp_highest_nonorm > 90      6grp_total > 56      4grp_total > 03      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 444
1 2 3 4 range
A 65.4
(-2.5)
P 		53.1
(-2.7)
A 		29.8
(-3)
A 		444.6
(3)
P 		415
B 45.4
(-2.5)
P 		15
(-3)
A 		33.2
(-2.7)
A 		367.7
(3)
P 		353
C 87.4
(-2.2)
P 		51.2
(-2.7)
A 		31.8
(-3)
P 		458.8
(3)
P 		427
avr 66.1
(-2.5) 		39.8
(-2.9) 		31.6
(-3) 		423.7
(3) 		392

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 123.6
(-0.1)
P 		123.9
(-0.1)
P 		179.6
(2.5)
P 		132.3
(0.3)
P 		61.3
(-3)
P 		191
(3)
P 		130
B 102.5
(-2.9)
P 		155.9
(0.5)
P 		101.2
(-3)
P 		101.8
(-3)
P 		128.7
(-1.2)
P 		194.2
(3)
P 		93
C 192.7
(3)
P 		141.4
(-1.4)
P 		139.7
(-1.5)
P 		126.8
(-2.6)
P 		122.6
(-3)
P 		164.1
(0.6)
P 		70
avr 139.6
(-0.3) 		140.4
(-0.2) 		140.2
(-0.3) 		120.3
(-1.8) 		104.2
(-3) 		183.1
(3) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 23.8
(-1.3)
P 		11.5
(-2.6)
A 		7.8
(-3)
A 		65.1
(3)
P 		57
B 14.5
(-2.1)
P 		3.7
(-3)
A 		7.7
(-2.7)
A 		78.4
(3)
P 		75
C 28.9
(-1.4)
P 		14.7
(-2.7)
A 		11.1
(-3)
P 		77.4
(3)
P 		66
avr 22.4
(-1.7) 		10
(-2.9) 		8.9
(-3) 		73.6
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 22.8
(-2.1)
P 		44.1
(2.5)
P 		39.1
(1.4)
P 		33.6
(0.2)
P 		18.5
(-3)
P 		46.5
(3)
P 		28
B 35.7
(-1.6)
P 		51.9
(0.4)
P 		24.6
(-3)
P 		27
(-2.7)
P 		52.3
(0.5)
P 		72.4
(3)
P 		48
C 71.4
(3)
P 		43.2
(-0.7)
P 		35.5
(-1.7)
P 		25.5
(-3)
P 		39.7
(-1.1)
P 		54.9
(0.8)
P 		46
avr 43.3
(0) 		46.4
(0.6) 		33.1
(-2.1) 		28.7
(-3) 		36.8
(-1.3) 		57.9
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177478_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12787
Sequence Type Exemplar sequence
Representative Public ID D1086.2 NCBI
Target Description D1086.2 /REP_DB=WormBase Gene ID /WP=CE09060 /TR=O17727 /GB=CAB04020.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074096(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074096 NCBI Caenorhabditis elegans D1086.2 (D1086.2) mRNA, complete cds. 11/11 None
SNAP00000017538 ENSEMBL cdna:SNAP chromosome:CEL140:V:14099749:14101217:-1 11/11 None
GENEFINDER00000017551 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14099749:14101217:-1 11/11 None
D1086.2 ENSEMBL cdna:known chromosome:CEL140:V:14099749:14101217:-1 gene:D1086.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14099748-14101217(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl D1086.2
Entrez Gene 183931 Entrez gene
SwissProt O17727 EMBL-EBI
WormBase CE09060 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04020 Hypothetical protein D1086.2 [Caenorhabditis elegans] ref|NP_506497.1| D1086.2 [Caenorhabditis elegans] 1.0E-101
blast CAE60989 Hypothetical protein CBG04725 [Caenorhabditis briggsae] 1.0E-62
Sequence
Target Sequence 
>C. ELEGANS:177478_AT
atgcagctcatatttcagctcttgatattcgagattttcgtgttttttggcgttcttgga
gccccatatcatgttcaagaagagtgttcaacaatggatcatcttattgatagacatctg
actgatttttccgaagctcttcaaagtgtgctggacgaaaaaaactcgtttgaaatgttg
agaggagaagatgaggataactttatgtttgtccagggatgtgaaaccattgaaagggac
ctaaatgttatcacttgcaagaaattaagagataagaatcggaatgttatcaagcaatgc
tatgagttggatcttgttggaacaccactgtcatactgtattcagagaaaacttaatgct
gacaaagtcgctaatcgttgtgtgaaaacatatttgaaatcaaaagacacacgtgagctt
aaacgtcttttcgacacaaatctgaaatgtttggtggaaggattcactagcctttgcgga
catgaagcggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAGCTCATATTTCAGCTCTTGA 1 45 13 Antisense
TTTTTTGGCGTTCTTGGAGCCCCAT 273 669 55 Antisense
TTGGAGCCCCATATCATGTTCAAGA 419 709 68 Antisense
ATCTGACTGATTTTTCCGAAGCTCT 71 33 128 Antisense
AGCTCTTCAAAGTGTGCTGGACGAA 704 79 147 Antisense
GGGACCTAAATGTTATCACTTGCAA 648 493 248 Antisense
GAGTTGGATCTTGTTGGAACACCAC 245 397 316 Antisense
GGAACACCACTGTCATACTGTATTC 687 527 331 Antisense
GTGAGCTTAAACGTCTTTTCGACAC 583 479 425 Antisense
GGTGGAAGGATTCACTAGCCTTTGC 329 497 465 Antisense
TAGCCTTTGCGGACATGAAGCGGGA 636 651 480 Antisense
Sequence Source Affymetrix Proprietary Database