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[177477_at]

Description F58A3.4 /REP_DB=WormBase Gene ID /WP=CE11358 /TR=Q93806 /GB=CAB02671.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 90      6grp_total > 44      4grp_total > 12      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 320
1 2 3 4 range
A 131.3
(-1.3)
P 		128.7
(-1.3)
P 		61.3
(-3)
P 		306
(3)
P 		245
B 157.9
(-1.4)
P 		93
(-3)
P 		92
(-3)
P 		334.6
(3)
P 		243
C 194.7
(-0.9)
P 		101.6
(-2.8)
P 		93.5
(-3)
P 		381.6
(3)
P 		288
avr 161.3
(-1.2) 		107.8
(-2.4) 		82.3
(-3) 		340.7
(3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 97.5
(-0.9)
P 		136.5
(2.1)
P 		149.1
(3)
P 		68.8
(-3)
P 		137.1
(2.1)
P 		100.8
(-0.6)
P 		80
B 145.6
(0.1)
P 		139.7
(-0.3)
P 		184.9
(3)
P 		103.4
(-3)
P 		142
(-0.2)
P 		149.1
(0.4)
P 		82
C 148.8
(3)
P 		129.1
(0.7)
P 		97.9
(-3)
P 		110.8
(-1.5)
P 		120.9
(-0.3)
P 		120.9
(-0.3)
P 		51
avr 130.6
(1.4) 		135.1
(1.9) 		144
(3) 		94.3
(-3) 		133.3
(1.7) 		123.6
(0.5) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 47.8
(3)
P 		27.9
(-0.8)
P 		16
(-3)
P 		44.8
(2.4)
P 		32
B 50.4
(0.5)
P 		23
(-2.8)
P 		21.3
(-3)
P 		71.3
(3)
P 		50
C 64.5
(3)
P 		29.2
(-3)
P 		32.6
(-2.4)
P 		64.3
(3)
P 		35
avr 54.2
(2) 		26.7
(-2.4) 		23.3
(-3) 		60.1
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 18
(-2.9)
P 		48.6
(3)
P 		32.5
(-0.1)
P 		17.4
(-3)
P 		41.4
(1.6)
P 		24.5
(-1.6)
P 		31
B 50.7
(1.6)
P 		46.5
(0.8)
P 		44.9
(0.5)
P 		27.4
(-3)
P 		57.7
(3)
P 		55.6
(2.6)
P 		30
C 55.2
(3)
P 		39.4
(0.1)
P 		24.9
(-2.5)
P 		22.3
(-3)
P 		39.1
(0.1)
P 		40.5
(0.3)
P 		33
avr 41.3
(1.8) 		44.8
(2.7) 		34.1
(-0) 		22.4
(-3) 		46.1
(3) 		40.2
(1.5) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177477_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16487
Sequence Type Exemplar sequence
Representative Public ID F58A3.4 NCBI
Target Description F58A3.4 /REP_DB=WormBase Gene ID /WP=CE11358 /TR=Q93806 /GB=CAB02671.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077439(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077439 NCBI Caenorhabditis elegans F58A3.4 (F58A3.4) mRNA, complete cds. 11/11 None
SNAP00000041416 ENSEMBL cdna:SNAP chromosome:CEL140:X:11009480:11010562:-1 11/11 None
GENEFINDER00000041422 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11009480:11010562:-1 11/11 None
F58A3.4 ENSEMBL cdna:known chromosome:CEL140:X:11009729:11010562:-1 gene:F58A3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11009728-11010562(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58A3.4
Entrez Gene 186483 Entrez gene
SwissProt Q93806 EMBL-EBI
WormBase CE11358 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE70767 Hypothetical protein CBG17518 [Caenorhabditis briggsae] 5.0E-72
blast CAB02671 Hypothetical protein F58A3.4 [Caenorhabditis elegans] ref|NP_509840.1| F58A3.4 [Caenorhabditis elegans] 3.0E-59
blast CAB02671 Hypothetical protein F58A3.4 [Caenorhabditis elegans] ref|NP_509840.1| F58A3.4 [Caenorhabditis elegans] 9.0E-55
blast CAE70767 Hypothetical protein CBG17518 [Caenorhabditis briggsae] 4.0E-54
blast CAE64599 Hypothetical protein CBG09354 [Caenorhabditis briggsae] 1.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 4.1E-19
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.9E-20
Sequence
Target Sequence 
>C. ELEGANS:177477_AT
atgctctatgattttgacgaccacaatttattcatcactcccaaaattgcttattttcca
acagcaatgggaggagcatcaagacacatgatggtgaacggaagtagtcacagaatcgct
gtcaagatcaagtgcagtgataatgaattgtttcgcgtatcgccggtatatacacttctt
gaaccaggaaatgcgcaaagattacagatagttcgcgatccagggccagcgaagacagat
aaaattgtggtgatttataagacaacatgcgcaagcagtgctagagatgcgttcgaatgc
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTCTATGATTTTGACGACCACA 447 41 13 Antisense
GACGACCACAATTTATTCATCACTC 656 379 28 Antisense
TATTCATCACTCCCAAAATTGCTTA 643 663 41 Antisense
GAACGGAAGTAGTCACAGAATCGCT 305 347 108 Antisense
AGAATCGCTGTCAAGATCAAGTGCA 563 75 124 Antisense
GAATTGTTTCGCGTATCGCCGGTAT 410 323 157 Antisense
ATCGCCGGTATATACACTTCTTGAA 501 37 171 Antisense
GATTACAGATAGTTCGCGATCCAGG 100 431 212 Antisense
CGCGATCCAGGGCCAGCGAAGACAG 93 255 226 Antisense
GACAACATGCGCAAGCAGTGCTAGA 19 367 273 Antisense
GTGCTAGAGATGCGTTCGAATGCGA 228 479 290 Antisense
Sequence Source Affymetrix Proprietary Database