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[177463_at]

Description ZK6.10 /REP_DB=WormBase Gene ID /WP=CE18447 /TR=O61874 /GB=AAG45576.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 60      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 81      6grp_total > 60      4grp_total > 60      6grp_highest_sub > 04      6grp_cgc > 94      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 675
1 2 3 4 range
A 525.7
(0.7)
P 		767.9
(3)
P 		624.8
(1.6)
P 		132.4
(-3)
P 		636
B 608.9
(2.6)
P 		641.1
(3)
P 		622.2
(2.8)
P 		92.9
(-3)
P 		548
C 534.7
(3)
P 		451.5
(1.5)
P 		483.1
(2)
P 		212.1
(-3)
P 		323
avr 556.4
(2.2) 		620.2
(3) 		576.7
(2.4) 		145.8
(-3) 		474

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 280
1 2 3 4 5 6 range
A 404.8
(3)
P 		287.2
(-1.3)
P 		249.3
(-2.7)
P 		337.1
(0.5)
P 		296.2
(-1)
P 		241.2
(-3)
P 		164
B 267.2
(-0.7)
P 		282.5
(-0.1)
P 		364.8
(3)
P 		338.4
(2)
P 		207.7
(-3)
P 		218.5
(-2.6)
P 		157
C 235.5
(-1.9)
P 		293.8
(-0.6)
P 		360.6
(0.8)
P 		462
(3)
P 		182.5
(-3)
P 		240.9
(-1.7)
P 		280
avr 302.5
(-0.1) 		287.8
(-0.6) 		324.9
(0.8) 		379.2
(3) 		228.8
(-3) 		233.5
(-2.8) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 191.4
(3)
P 		166.7
(2.1)
P 		163.3
(2)
P 		19.4
(-3)
P 		172
B 194.2
(3)
P 		158.3
(1.8)
P 		144.2
(1.3)
P 		19.8
(-3)
P 		174
C 177
(3)
P 		129.8
(1)
P 		168.4
(2.6)
P 		35.8
(-3)
P 		141
avr 187.5
(3) 		151.6
(1.7) 		158.6
(1.9) 		25
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 74.6
(-0.5)
P 		102.2
(3)
P 		54.3
(-3)
P 		85.5
(0.9)
P 		89.4
(1.4)
P 		58.7
(-2.4)
P 		48
B 93
(2.5)
P 		94
(3)
P 		88.6
(0.4)
P 		89.6
(0.9)
P 		84.4
(-1.6)
P 		81.5
(-3)
P 		13
C 87.3
(2)
P 		89.6
(2.4)
P 		91.8
(2.8)
P 		92.9
(3)
P 		59
(-3)
P 		80.6
(0.8)
P 		34
avr 85
(0.2) 		95.3
(3) 		78.2
(-1.7) 		89.3
(1.3) 		77.6
(-1.9) 		73.6
(-3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177463_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15230
Sequence Type Exemplar sequence
Representative Public ID ZK6.10 NCBI
Target Description ZK6.10 /REP_DB=WormBase Gene ID /WP=CE18447 /TR=O61874 /GB=AAG45576.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070792
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000041445 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:410468:414455:-1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070792 6/11 Cross Hyb Matching Probes A 
SNAP00000041443 6/11 Cross Hyb Matching Probes None
ZK6.10 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:411803-414545(-) 93.43 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG45576 Downstream of daf-16 (regulated by daf-16) protein 19 [Caenorhabditis elegans] ref|NP_503193.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-19) [Caenorhabditis elegans] 0.0
blast T33206 hypothetical protein ZK6.10 - Caenorhabditis elegans 0.0
blast AAG45577 Hypothetical protein ZK6.11a [Caenorhabditis elegans] ref|NP_001024314.1| ZK6.11a [Caenorhabditis elegans] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 3.1E-22
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000041445 1 394-416
Sequence
Target Sequence 
>C. ELEGANS:177463_AT
gagccaggatatacgagcatcgattcgacaaccacaacaaatgtcaccacaactggattc
tatatgaagcccatgggcacagtttctagtaccattactgttaatatcaagagagatact
agatatcctggagcttctggcgtaaatgttcgcgggtatgtcccaacagggggaaaagtc
acagtaggattttataatggagcatcaaaatatgaacaatcagcgccgccaaatgaattc
ttgtctgcatggagcattccatatattgcaccgatgttcaaagtttcatctacatcgtca
aactcattggctgagcagtactttgtgcaatactttgtagcccgactaagcctgagcctg
agcctaagccaaaacctgagcctacgcccaaatttaacacccaagcctaagtttaagact
atgtctaagcctagcctaaacccaagtttttttttgaaaatgttgaaaattcgatgtatt
ccgcatttcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCAGGATATACGAGCATCGATT 352 385 709 Antisense
AGCATCGATTCGACAACCACAACAA 520 77 724 Antisense
AAATGTCACCACAACTGGATTCTAT 233 119 747 Antisense
GCCCATGGGCACAGTTTCTAGTACC 222 285 777 Antisense
ATATCCTGGAGCTTCTGGCGTAAAT 278 17 831 Antisense
AAATGTTCGCGGGTATGTCCCAACA 548 119 852 Antisense
TACTTTGTAGCCCGACTAAGCCTGA 679 369 1039 Antisense
CTAAGCCAAAACCTGAGCCTACGCC 58 225 1072 Antisense
TAAGACTATGTCTAAGCCTAGCCTA 390 637 1122 Antisense
TCTAAGCCTAGCCTAAACCCAAGTT 671 613 1132 Antisense
AATTCGATGTATTCCGCATTTCAGA 151 175 1176 Antisense
Sequence Source Affymetrix Proprietary Database