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[177454_at]

Description W02B8.4 /REP_DB=WormBase Gene ID /WP=CE20133 /TR=Q9XVH2 /GB=CAB03460.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 64      4grp_total > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 16.3
(-1.2)
A 		10
(-2)
A 		1.6
(-3)
A 		50.5
(3)
A 		49
B 2.9
(1.1)
A 		3
(1.3)
A 		3.8
(3)
A 		1
(-3)
A 		3
C 2.9
(-3)
A 		2.8
(-3)
A 		7.5
(-2)
A 		30.9
(3)
A 		28
avr 7.4
(-2.2) 		5.3
(-2.7) 		4.3
(-3) 		27.5
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 1.1
(-2.9)
A 		3.8
(-2.3)
A 		0.9
(-3)
A 		24.6
(3)
A 		4.8
(-2)
A 		1.7
(-2.8)
A 		24
B 0.4
(-3)
A 		10.9
(-0.4)
A 		24.3
(3)
A 		2.3
(-2.5)
A 		1.9
(-2.6)
A 		22.4
(2.5)
A 		24
C 3.1
(3)
A 		1.3
(-3)
A 		1.5
(-2.3)
A 		2.4
(0.7)
A 		1.5
(-2.3)
A 		2.7
(1.7)
A 		2
avr 1.5
(-3) 		5.3
(-0.3) 		8.9
(2.3) 		9.8
(3) 		2.7
(-2.1) 		8.9
(2.3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 5.9
(1.7)
A 		2.2
(-1.5)
A 		0.4
(-3)
A 		7.4
(3)
A 		7
B 0.9
(3)
A 		0.7
(1.3)
A 		0.9
(3)
A 		0.2
(-3)
A 		1
C 0.9
(-2.9)
A 		0.8
(-3)
A 		2.6
(-0.5)
A 		5.2
(3)
A 		4
avr 2.6
(-0.3) 		1.2
(-3) 		1.3
(-2.8) 		4.3
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.2
(-3)
A 		1.4
(-1.8)
A 		0.2
(-3)
A 		6.2
(3)
A 		1.5
(-1.7)
A 		0.4
(-2.8)
A 		6
B 0.1
(-3)
A 		3.6
(-0.4)
A 		5.9
(1.2)
A 		0.6
(-2.6)
A 		0.8
(-2.5)
A 		8.3
(3)
A 		8
C 1.1
(3)
A 		0.4
(-3)
A 		0.4
(-3)
A 		0.5
(-2.1)
A 		0.5
(-2.1)
A 		0.9
(1.3)
A 		1
avr 0.5
(-3) 		1.8
(-0.1) 		2.2
(0.8) 		2.4
(1.2) 		0.9
(-2.1) 		3.2
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177454_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3060
Sequence Type Exemplar sequence
Representative Public ID W02B8.4 NCBI
Target Description W02B8.4 /REP_DB=WormBase Gene ID /WP=CE20133 /TR=Q9XVH2 /GB=CAB03460.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064515
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
W02B8.4 ENSEMBL cdna:known chromosome:CEL140:II:13920447:13924616:1 gene:W02B8.4 None
SNAP00000004894 ENSEMBL cdna:SNAP chromosome:CEL140:II:13920504:13924616:1 None
NM_064515 NCBI Caenorhabditis elegans W02B8.4 (W02B8.4) mRNA, complete cds. None
GENEFINDER00000004900 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13920447:13929814:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027221 4/11 Cross Hyb Matching Probes None
SNAP00000004895 4/11 Cross Hyb Matching Probes None
W02B8.6 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13921685-13923591(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W02B8.4
Entrez Gene 189109 Entrez gene
SwissProt Q9XVH2 EMBL-EBI
WormBase CE37935 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03460 Hypothetical protein W02B8.4 [Caenorhabditis elegans] ref|NP_496916.2| W02B8.4 [Caenorhabditis elegans] 0.0
blast CAB03459 Hypothetical protein W02B8.3 [Caenorhabditis elegans] ref|NP_496915.1| W02B8.3 [Caenorhabditis elegans] 0.0
blast CAE73323 Hypothetical protein CBG20752 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Pfam IPR006954 EMBL-EBI Protein of unknown function DUF644 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496916.2 4 586-608,615-637,641-658,665-687
W02B8.4 4 586-608,615-637,641-658,665-687
SNAP00000004894 4 529-551,558-580,584-601,608-630
GENEFINDER00000004900 4 575-597,604-626,630-647,654-676
Sequence
Target Sequence 
>C. ELEGANS:177454_AT
cttctttcaaatcacttttaggctctgcgatgactgacaccgacacccagaacttcttag
atttactggtagaagccactggagtagctgaggccgtggaagacgctgagcacaaattga
ttgatgctctgagaaagaaagatgatgcgatgggaagaggtcctgatgaaagatttccat
cagaagcgaggaagatagagttaatggagaagttggataaaacctattcaattgaacaac
tgaaagatatgaatcaaaccgggtatactgtattgacaccgaagcaaatgcaactgatgt
acggaaaacagtctccgttcaagaatccaaagttattgaggacttataagaatatgtcaa
gagcagagattcatagatcgattcacagtacgattaaggatgtggcagatgaaaagttga
aattcgagcattattttttgctacaatcaatggagatagtaacttttgctccagaagaaa
ttagtcagaacctgaaaattggcgccaaacttatggtttatatcgtgccgaagtttaacc
caat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTTTCAAATCACTTTTAGGCTC 174 211 707 Antisense
ACTTTTAGGCTCTGCGATGACTGAC 313 103 720 Antisense
GAAGCCACTGGAGTAGCTGAGGCCG 367 343 778 Antisense
AGCTGAGGCCGTGGAAGACGCTGAG 21 81 792 Antisense
AAGAGGTCCTGATGAAAGATTTCCA 279 151 861 Antisense
GAAAGATTTCCATCAGAAGCGAGGA 180 361 874 Antisense
ATCAAACCGGGTATACTGTATTGAC 530 27 959 Antisense
GATAGTAACTTTTGCTCCAGAAGAA 279 425 1161 Antisense
CAGAACCTGAAAATTGGCGCCAAAC 68 205 1192 Antisense
AAACTTATGGTTTATATCGTGCCGA 51 129 1213 Antisense
ATATCGTGCCGAAGTTTAACCCAAT 662 19 1226 Antisense
Sequence Source Affymetrix Proprietary Database