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[177451_at]

Description ZC373.6 /REP_DB=WormBase Gene ID /WP=CE02379 /TR=Q23262 /GB=CAA88978.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 26      6grp_cgc > 88      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 3
(-3)
A 		20.5
(-1.4)
A 		24.7
(-1)
A 		67.4
(3)
A 		64
B 12.5
(-1.2)
A 		38.2
(3)
P 		1.6
(-3)
A 		29.7
(1.6)
A 		37
C 19.4
(-2.5)
A 		43.6
(1.3)
A 		16
(-3)
A 		54.8
(3)
A 		39
avr 11.6
(-3) 		34.1
(0.5) 		14.1
(-2.6) 		50.6
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 376
1 2 3 4 5 6 range
A 35.5
(-2.7)
A 		20.5
(-3)
A 		23.8
(-2.9)
A 		177.2
(0.1)
P 		323.3
(3)
P 		56.3
(-2.3)
P 		303
B 34.9
(-2.8)
A 		24.4
(-3)
A 		37
(-2.8)
A 		151.2
(-0.9)
P 		395.2
(3)
P 		63.9
(-2.4)
P 		371
C 19.4
(-3)
A 		39.4
(-2.6)
A 		21.5
(-3)
A 		149.8
(-0.3)
P 		310.2
(3)
P 		41.9
(-2.5)
P 		291
avr 29.9
(-3) 		28.1
(-3) 		27.4
(-3) 		159.4
(-0.5) 		342.9
(3) 		54
(-2.5) 		316
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.1
(-3)
A 		4.4
(-0.8)
A 		6.5
(0.7)
A 		9.9
(3)
A 		9
B 4
(-0.6)
A 		9.4
(3)
P 		0.4
(-3)
A 		6.3
(0.9)
A 		9
C 6.4
(-2.3)
A 		12.5
(3)
A 		5.6
(-3)
A 		9.2
(0.1)
A 		7
avr 3.8
(-3) 		8.8
(3) 		4.2
(-2.5) 		8.5
(2.6) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 6.5
(-2.9)
A 		7.3
(-2.9)
A 		5.2
(-3)
A 		44.9
(-0.4)
P 		97.6
(3)
P 		13.7
(-2.4)
P 		92
B 12.1
(-2.8)
A 		8.1
(-3)
A 		9
(-3)
A 		40
(-1.7)
P 		160.6
(3)
P 		23.8
(-2.4)
P 		153
C 7.2
(-2.9)
A 		12
(-2.6)
A 		5.5
(-3)
A 		30.1
(-1.4)
P 		100.4
(3)
P 		14
(-2.5)
P 		95
avr 8.6
(-2.9) 		9.1
(-2.9) 		6.6
(-3) 		38.3
(-1.3) 		119.5
(3) 		17.2
(-2.4) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177451_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16043
Sequence Type Exemplar sequence
Representative Public ID ZC373.6 NCBI
Target Description ZC373.6 /REP_DB=WormBase Gene ID /WP=CE02379 /TR=Q23262 /GB=CAA88978.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077290(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077290 NCBI Caenorhabditis elegans Dauer or Aging adult Overexpression family member (dao-4) (dao-4) mRNA, complete cds. 11/11 None
SNAP00000017667 ENSEMBL cdna:SNAP chromosome:CEL140:X:10075229:10076135:1 11/11 None
GENEFINDER00000017672 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10075229:10076135:1 11/11 None
ZC373.6 ENSEMBL cdna:known chromosome:CEL140:X:10075229:10076135:1 gene:ZC373.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10075228-10076135(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dao-4
Ensembl ZC373.6
Entrez Gene 191146 Entrez gene
SwissProt Q23262 EMBL-EBI
WormBase CE02379 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88978 Hypothetical protein ZC373.6 [Caenorhabditis elegans] ref|NP_509691.1| Dauer or Aging adult Overexpression family member (dao-4) [Caenorhabditis elegans] 1.0E-102
blast CAE58776 Hypothetical protein CBG01971 [Caenorhabditis briggsae] 4.0E-76
Sequence
Target Sequence 
>C. ELEGANS:177451_AT
ttctcattaccgtttgttatttatcatctacagatgcttatttatttgaccctaaggcag
tcgatgctcttcttagagaaattcgagcaaggttacaggcgacaggcaaagagcctcacg
caaggaaaattgagcaggaaactgaagaaattaaaaaagaagagctaatgcaagctgagg
atgtggaaggttctggatcaggagaagaaattgaaggatccggagaagttatcagtacaa
taaccggagtaccaattatggacaatgaagagaagaaagacttgaaaccagaaaatttcc
caagacccgaaccaatttttgataaatatggaaatttgaaaagcaaggacaaacttgaag
cgttgacatattccaactttaagaaacaagcacctgcgaccttgcaagatcactacaatt
taaatcctactggcacattacaaatgcttcaaggactcgatattcatggaggctctggtg
gataccatagagcactttccggtggatatcttcctccatcaacatatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCATTACCGTTTGTTATTTATC 314 691 38 Antisense
ACAGATGCTTATTTATTTGACCCTA 128 109 67 Antisense
GACCCTAAGGCAGTCGATGCTCTTC 473 381 85 Antisense
GAGCAAGGTTACAGGCGACAGGCAA 350 391 122 Antisense
GCGACAGGCAAAGAGCCTCACGCAA 564 289 136 Antisense
AAGAAACAAGCACCTGCGACCTTGC 56 153 418 Antisense
GCGACCTTGCAAGATCACTACAATT 434 289 433 Antisense
TTTAAATCCTACTGGCACATTACAA 482 671 456 Antisense
GGACTCGATATTCATGGAGGCTCTG 598 523 490 Antisense
GATACCATAGAGCACTTTCCGGTGG 38 423 518 Antisense
GATATCTTCCTCCATCAACATATGA 100 427 542 Antisense
Sequence Source Affymetrix Proprietary Database