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[177443_at]

Description C54G4.2 /REP_DB=WormBase Gene ID /WP=CE05508 /TR=Q18847 /GB=CAA99815.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 17      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 4
(-3)
A 		19.2
(-1.7)
A 		36.3
(-0.3)
A 		75.4
(3)
A 		71
B 2.4
(-3)
A 		2.9
(-2.9)
A 		2.5
(-3)
A 		28.2
(3)
A 		26
C 4.6
(-3)
A 		34.8
(2.2)
A 		12.7
(-1.6)
A 		39.4
(3)
A 		35
avr 3.7
(-3) 		19
(-0.9) 		17.2
(-1.2) 		47.7
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 3.3
(-2.7)
A 		2.4
(-3)
A 		4.8
(-2.3)
A 		6.8
(-1.7)
A 		20.2
(2.3)
A 		22.7
(3)
A 		20
B 2.4
(-3)
A 		4
(-2.8)
A 		28.5
(0.7)
A 		34.5
(1.6)
A 		18.5
(-0.7)
A 		44.2
(3)
A 		42
C 4.1
(-3)
A 		7.5
(-2.1)
A 		6.7
(-2.3)
A 		11.3
(-1.1)
A 		21.1
(1.5)
A 		27
(3)
A 		23
avr 3.3
(-3) 		4.6
(-2.7) 		13.3
(-0.9) 		17.5
(0) 		19.9
(0.6) 		31.3
(3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1.4
(-3)
A 		4.2
(-1.3)
A 		9.5
(2.1)
A 		11
(3)
A 		10
B 0.8
(-2.8)
A 		0.7
(-2.9)
A 		0.6
(-3)
A 		6
(3)
A 		5
C 1.5
(-3)
A 		10
(3)
A 		4.4
(-1)
A 		6.7
(0.7)
A 		9
avr 1.2
(-3) 		5
(0.4) 		4.8
(0.2) 		7.9
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 0.6
(-3)
A 		0.8
(-2.8)
A 		1
(-2.6)
A 		1.7
(-1.8)
A 		6.1
(3)
A 		5.5
(2.3)
A 		6
B 0.8
(-3)
A 		1.3
(-2.8)
A 		6.9
(-0.7)
A 		9.1
(0.2)
A 		7.5
(-0.4)
A 		16.5
(3)
A 		16
C 1.5
(-3)
A 		2.3
(-2.4)
A 		1.7
(-2.8)
A 		2.3
(-2.4)
A 		6.8
(1.2)
A 		9
(3)
A 		8
avr 1
(-3) 		1.5
(-2.7) 		3.2
(-1.6) 		4.4
(-0.8) 		6.8
(0.7) 		10.3
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177443_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2335
Sequence Type Exemplar sequence
Representative Public ID C54G4.2 NCBI
Target Description C54G4.2 /REP_DB=WormBase Gene ID /WP=CE05508 /TR=Q18847 /GB=CAA99815.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059802(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059802 NCBI Caenorhabditis elegans C54G4.2 (C54G4.2) mRNA, complete cds. 11/11 None
SNAP00000028897 ENSEMBL cdna:SNAP chromosome:CEL140:I:8012698:8017155:1 11/11 None
GENEFINDER00000028907 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8014776:8020314:1 11/11 None
C54G4.2 ENSEMBL cdna:known chromosome:CEL140:I:8014776:8017238:1 gene:C54G4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8014706-8017086(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54G4.2
Entrez Gene 172580 Entrez gene
SwissProt Q18847 EMBL-EBI
WormBase CE05508 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99815 Hypothetical protein C54G4.2 [Caenorhabditis elegans] ref|NP_492203.1| C54G4.2 [Caenorhabditis elegans] 0.0
blast CAE71724 Hypothetical protein CBG18702 [Caenorhabditis briggsae] 2.0E-75
blast CAA86759 Hypothetical protein C09G5.7 [Caenorhabditis elegans] ref|NP_496312.1| C09G5.7 [Caenorhabditis elegans] sp|Q09458|YQ37_CAEEL Hypothetical protein C09F5.7 in chromosome II 5.0E-51
Sequence
Target Sequence 
>C. ELEGANS:177443_AT
atcctgagctactagcggcggttgctgaagacggactgaagattgaggatggaaagcaat
ggattgggctggtcgaggattatacaacagggaaaatggatgatgggattattgaggaga
gcagagaccaactcaacccatatgacacgatggctgcgctgcaagatcgatctgagaagt
tcttcagaatgaactcggtgaattactacaccatgtcgaatttgttgactttgagtaaag
aagcgattgaccgatttgccgctcaggagcagacaacagcagcggagcgtcgtaggaagg
aagaagctctgaagacatcgaaagaaaacggcaatgaaaatgagaagacatgtgtggtga
cgtcgattacgttcaaaatggaaagcacgtttagcatcagctatgatagacgtcgcccgg
tgctctcgattcaaaagttccgtaaaaagtacaaaaaacgtctccgctcagcgatggatc
aggcaagaagaggttcacgagagggctcacgggagcctgtttctcgtgaaggatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCTGAGCTACTAGCGGCGGTTGC 190 37 707 Antisense
CGGCGGTTGCTGAAGACGGACTGAA 313 241 722 Antisense
GGATTGGGCTGGTCGAGGATTATAC 544 505 767 Antisense
AGAGCAGAGACCAACTCAACCCATA 368 69 824 Antisense
TATGACACGATGGCTGCGCTGCAAG 276 655 847 Antisense
GAAGCGATTGACCGATTTGCCGCTC 335 343 946 Antisense
ACAGCAGCGGAGCGTCGTAGGAAGG 522 107 982 Antisense
ATCAGCTATGATAGACGTCGCCCGG 372 29 1102 Antisense
AAAACGTCTCCGCTCAGCGATGGAT 312 131 1161 Antisense
GGTTCACGAGAGGGCTCACGGGAGC 115 507 1198 Antisense
GGGAGCCTGTTTCTCGTGAAGGATC 427 495 1217 Antisense
Sequence Source Affymetrix Proprietary Database