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[177438_at]

Description F58D5.2 /REP_DB=WormBase Gene ID /WP=CE25018 /TR=Q9NLD2 /GB=CAB70237.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 19      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 50      6grp_total > 29      4grp_total > 46      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 481
1 2 3 4 range
A 550.6
(3)
P 		347.1
(-0.9)
P 		235.8
(-3)
P 		296.7
(-1.8)
P 		315
B 670.1
(3)
P 		283.4
(-2)
P 		203.4
(-3)
P 		219.5
(-2.8)
P 		467
C 625.4
(3)
P 		402.3
(-0.1)
P 		189.4
(-3)
P 		258.2
(-2.1)
P 		436
avr 615.4
(3) 		344.3
(-1) 		209.5
(-3) 		258.1
(-2.3) 		406

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 675
1 2 3 4 5 6 range
A 711.8
(3)
P 		477.8
(0.2)
P 		364.7
(-1.1)
P 		344.7
(-1.4)
P 		652.8
(2.3)
P 		208.5
(-3)
P 		503
B 861.4
(3)
P 		619.3
(0.8)
P 		220.3
(-2.7)
P 		368
(-1.4)
P 		747.6
(2)
P 		186.2
(-3)
P 		675
C 771.7
(3)
P 		450.4
(-0.4)
P 		372.5
(-1.2)
P 		346.6
(-1.5)
P 		474.1
(-0.1)
P 		202.8
(-3)
P 		569
avr 781.6
(3) 		515.8
(0.3) 		319.2
(-1.8) 		353.1
(-1.4) 		624.8
(1.4) 		199.2
(-3) 		582
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 200.5
(3)
P 		75.3
(-1.8)
P 		61.6
(-2.3)
P 		43.4
(-3)
P 		157
B 213.7
(3)
P 		70
(-2.2)
P 		47.1
(-3)
P 		46.8
(-3)
P 		167
C 207
(3)
P 		115.7
(-0.4)
P 		66
(-2.2)
P 		43.5
(-3)
P 		164
avr 207.1
(3) 		87
(-1.4) 		58.2
(-2.5) 		44.6
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 131.2
(0.3)
P 		169.9
(1.9)
P 		79.4
(-1.8)
P 		87.4
(-1.5)
P 		197
(3)
P 		50.8
(-3)
P 		146
B 299.7
(2.9)
P 		206.1
(0.7)
P 		53.5
(-3)
P 		97.5
(-1.9)
P 		303.7
(3)
P 		69.4
(-2.6)
P 		250
C 286
(3)
P 		137.4
(-1.1)
P 		94.8
(-2.3)
P 		69.7
(-3)
P 		153.4
(-0.6)
P 		67.9
(-3)
P 		218
avr 239
(3) 		171.1
(0.7) 		75.9
(-2.6) 		84.9
(-2.2) 		218
(2.3) 		62.7
(-3) 		176
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177438_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1377
Sequence Type Exemplar sequence
Representative Public ID F58D5.2 NCBI
Target Description F58D5.2 /REP_DB=WormBase Gene ID /WP=CE25018 /TR=Q9NLD2 /GB=CAB70237.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026345(10), NM_001026346(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026345 NCBI Caenorhabditis elegans F58D5.2a (F58D5.2) mRNA, complete cds. 10/11 None
NM_001026346 NCBI Caenorhabditis elegans F58D5.2b (F58D5.2) mRNA, complete cds. 10/11 None
GENEFINDER00000034708 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12074167:12076782:-1 11/11 None
F58D5.2a ENSEMBL cdna:known chromosome:CEL140:I:12074167:12076782:-1 gene:F58D5.2 10/11 A 
F58D5.2b.2 ENSEMBL cdna:known chromosome:CEL140:I:12074167:12076755:-1 gene:F58D5.2 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034701 8/11 Cross Hyb Matching Probes None
F58D5.2b.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12074097-12076713(-) 91.01 100.0
Public Domain and Genome References
Ensembl F58D5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70237 Hypothetical protein F58D5.2a [Caenorhabditis elegans] ref|NP_001021516.1| F58D5.2a [Caenorhabditis elegans] 6.0E-99
blast CAB70237 Hypothetical protein F58D5.2a [Caenorhabditis elegans] ref|NP_001021516.1| F58D5.2a [Caenorhabditis elegans] 1.0E-94
blast CAE69604 Hypothetical protein CBG15833 [Caenorhabditis briggsae] 2.0E-75
blast CAE69604 Hypothetical protein CBG15833 [Caenorhabditis briggsae] 1.0E-71
blast CAE11315 Hypothetical protein F58D5.2b [Caenorhabditis elegans] ref|NP_001021517.1| F58D5.2b [Caenorhabditis elegans] 5.0E-32
blast CAB70237 Hypothetical protein F58D5.2a [Caenorhabditis elegans] ref|NP_001021516.1| F58D5.2a [Caenorhabditis elegans] 5.0E-32
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021516.1 4 44-63,73-95,108-130,145-167
NP_001021517.1 1 20-42
F58D5.2a 4 44-63,73-95,108-130,145-167
F58D5.2b.2 1 20-42
GENEFINDER00000034708 4 72-89,99-121,134-156,171-193
Sequence
Target Sequence 
>C. ELEGANS:177438_AT
tcactttggacactttttacgcctcacagtgcagaggaatcaccaaaactgtttgcattg
taaggggtgtaacatcgatagagatagtgaaaaattgtcaactgataaaatgtagaaaat
ttaaaattgaatattttgcgatgtgcttcttcgcaatagtattggtatacgcttctcagc
ctgtaaatgacgagcgaggccctgcgctctttttctgtgctctcggtatcttggtcagtt
ttggagtcctcttggagtacacatttatgctcaacactcatatcactgccacgtgtgggt
ttattttggaagctcttctgtatgtgctcgtttcggtgggcctccttgtgtctgccatct
tgatgtgggtgttttgtgcggctcattgggctgaaatgaatcccgaatgggctacgatgc
cagcactggctacggctgctctattcttgtgttcggctctttatttcatagaaatctgtg
tcttgctcaatgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACTTTGGACACTTTTTACGCCTC 575 581 107 Antisense
ACTTTTTACGCCTCACAGTGCAGAG 252 101 118 Antisense
GAATATTTTGCGATGTGCTTCTTCG 147 327 235 Antisense
AGTATTGGTATACGCTTCTCAGCCT 512 63 264 Antisense
TACGCTTCTCAGCCTGTAAATGACG 154 649 274 Antisense
AAATGACGAGCGAGGCCCTGCGCTC 412 119 291 Antisense
CTCTCGGTATCTTGGTCAGTTTTGG 135 231 326 Antisense
GTTTTGGAGTCCTCTTGGAGTACAC 708 447 344 Antisense
GTACACATTTATGCTCAACACTCAT 394 461 363 Antisense
CACTCATATCACTGCCACGTGTGGG 88 197 381 Antisense
GAAATCTGTGTCTTGCTCAATGATG 309 363 577 Antisense
Sequence Source Affymetrix Proprietary Database