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[177429_s_at]

Description F54E4.3 /REP_DB=WormBase Gene ID /WP=CE11078 /TR=Q93786 /GB=CAB01917.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 30      6grp_total > 24      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1332
1 2 3 4 range
A 1645.3
(3)
P 		738.3
(-1.8)
P 		516.4
(-3)
P 		510.6
(-3)
P 		1135
B 1749.1
(3)
P 		640.8
(-2.1)
P 		470.5
(-2.9)
P 		456.1
(-3)
P 		1293
C 1613.5
(3)
P 		695.7
(-1.6)
P 		561.6
(-2.3)
P 		417.3
(-3)
P 		1196
avr 1669.3
(3) 		691.6
(-1.9) 		516.2
(-2.7) 		461.3
(-3) 		1208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 838
1 2 3 4 5 6 range
A 1216.4
(3)
P 		1062.6
(1.9)
P 		859.4
(0.3)
P 		733.2
(-0.6)
P 		509.7
(-2.2)
P 		408.5
(-3)
P 		808
B 1174.6
(3)
P 		991.6
(1.5)
P 		923.2
(0.9)
P 		604.7
(-1.8)
P 		481.9
(-2.8)
P 		462
(-3)
P 		713
C 1246.8
(3)
P 		1076.5
(1.6)
P 		809.5
(-0.5)
P 		704.3
(-1.4)
P 		504.6
(-3)
P 		553
(-2.6)
P 		742
avr 1212.6
(3) 		1043.6
(1.6) 		864
(0.2) 		680.7
(-1.3) 		498.7
(-2.8) 		474.5
(-3) 		738
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 529
1 2 3 4 range
A 599.1
(3)
P 		160.3
(-2)
P 		135
(-2.3)
P 		74.7
(-3)
P 		524
B 557.8
(3)
P 		158.3
(-2.2)
P 		109
(-2.8)
P 		97.2
(-3)
P 		461
C 534.1
(3)
P 		200
(-1.3)
P 		195.7
(-1.4)
P 		70.4
(-3)
P 		464
avr 563.7
(3) 		172.9
(-1.9) 		146.6
(-2.2) 		80.8
(-3) 		483

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 224.2
(-0.3)
P 		377.9
(3)
P 		187.2
(-1.1)
P 		186
(-1.1)
P 		153.8
(-1.8)
P 		99.5
(-3)
P 		278
B 408.7
(3)
P 		330.1
(1.1)
P 		224.2
(-1.5)
P 		160.2
(-3)
P 		195.8
(-2.1)
P 		172.3
(-2.7)
P 		249
C 462.1
(3)
P 		328.5
(0.5)
P 		206
(-1.8)
P 		141.6
(-3)
P 		163.3
(-2.6)
P 		185.1
(-2.2)
P 		321
avr 365
(3) 		345.5
(2.4) 		205.8
(-1.5) 		162.6
(-2.7) 		171
(-2.5) 		152.3
(-3) 		213
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177429_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17514
Sequence Type Exemplar sequence
Representative Public ID F54E4.3 NCBI
Target Description F54E4.3 /REP_DB=WormBase Gene ID /WP=CE11078 /TR=Q93786 /GB=CAB01917.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078032 NCBI Caenorhabditis elegans F54E4.3 (F54E4.3) mRNA, complete cds. 11/11 None
GENEFINDER00000032274 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14632652:14636326:-1 9/11 None
F54E4.3 ENSEMBL cdna:known chromosome:CEL140:X:14632049:14636326:-1 gene:F54E4.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032270 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14632264-14636326(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54E4.3
Entrez Gene 181559 Entrez gene
SwissProt Q93786 EMBL-EBI
WormBase CE11078 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01917 Hypothetical protein F54E4.3 [Caenorhabditis elegans] ref|NP_510433.1| F54E4.3 [Caenorhabditis elegans] 1.0E-147
blast CAB01917 Hypothetical protein F54E4.3 [Caenorhabditis elegans] ref|NP_510433.1| F54E4.3 [Caenorhabditis elegans] 1.0E-133
blast CAE63209 Hypothetical protein CBG07564 [Caenorhabditis briggsae] 1.0E-112
blast CAE63209 Hypothetical protein CBG07564 [Caenorhabditis briggsae] 1.0E-109
Sequence
Target Sequence 
>C. ELEGANS:177429_S_AT
ttgtacaagtttccaacaccgtctccgacttgctcactcatagaaaagattcaggatcat
tcttgagaagttattatcttctcacgtattcttgctctgtagaaggccaagagattttga
gcaagcatcgtgactgcttgaagtccgaaaaaattggtgagatgacactttccgctggta
cataccttagtgagaagtttttggagcactcgcaagatgacgtctgcgaggcagttaacc
gaaaattacaagagtacatttcgctcttggcggtctttgcaggacttcatgccgacaaat
tggacagttgcgttttggactgcaagattccagagaacaacgacgaggagagcaaagaag
aagttgctcttacatctgacgctcaagatgaagaaaacctcgaacagcagcaatccgaac
aggatgccctccgaccagctgactcgtccagcatagccttttccctattttcaattatat
tttatgatcatcaattgtatgcatttgtggtgaatttttcttcaaattccaagtcaagtc
ccgatttcttctcccatatgagctttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTACAAGTTTCCAACACCGTCTC 666 671 278 Antisense
CTCCGACTTGCTCACTCATAGAAAA 219 235 300 Antisense
TTCTCACGTATTCTTGCTCTGTAGA 651 691 356 Antisense
ACACTTTCCGCTGGTACATACCTTA 591 107 442 Antisense
GGAGCACTCGCAAGATGACGTCTGC 577 519 480 Antisense
AGTACATTTCGCTCTTGGCGGTCTT 37 63 530 Antisense
GGACTTCATGCCGACAAATTGGACA 253 525 559 Antisense
GAAAACCTCGAACAGCAGCAATCCG 658 351 670 Antisense
CAGCAATCCGAACAGGATGCCCTCC 612 203 685 Antisense
TCCAAGTCAAGTCCCGATTTCTTCT 304 585 805 Antisense
GATTTCTTCTCCCATATGAGCTTTG 201 429 820 Antisense
Sequence Source Affymetrix Proprietary Database