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[177420_s_at]

Description K09E4.2 /REP_DB=WormBase Gene ID /WP=CE25052 /TR=Q9NAP5 /GB=CAB70171.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 29      6grp_highest_nonorm > 90      6grp_total > 29      4grp_total > 52      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 105.6
(3)
P 		74.5
(-0.4)
P 		82.4
(0.4)
P 		51.1
(-3)
A 		55
B 78.1
(0.9)
P 		111.4
(3)
P 		70
(0.3)
P 		17.7
(-3)
A 		94
C 126.9
(1.5)
P 		143.2
(3)
P 		82
(-2.6)
P 		78
(-3)
M 		65
avr 103.5
(2.4) 		109.7
(3) 		78.1
(-0.1) 		48.9
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 93
(0.6)
P 		94.8
(0.8)
P 		65.5
(-3)
P 		76
(-1.6)
P 		111.7
(3)
P 		104
(2)
P 		46
B 89.2
(-2.5)
P 		95.6
(-0.8)
P 		109.8
(3)
P 		99
(0.1)
P 		105.1
(1.7)
P 		87.3
(-3)
P 		23
C 120
(3)
P 		80.6
(-0.7)
P 		92.2
(0.4)
P 		56.2
(-3)
P 		82.9
(-0.5)
P 		82.2
(-0.6)
P 		64
avr 100.7
(3) 		90.3
(0.4) 		89.2
(0.1) 		77.1
(-3) 		99.9
(2.8) 		91.2
(0.6) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 38.4
(3)
P 		16.2
(-1.3)
P 		21.6
(-0.3)
P 		7.5
(-3)
A 		31
B 24.9
(2.3)
P 		27.5
(3)
P 		16.2
(0.1)
P 		3.8
(-3)
A 		24
C 42
(3)
P 		41.2
(2.8)
P 		28.6
(0.2)
P 		13.2
(-3)
M 		29
avr 35.1
(3) 		28.3
(1.5) 		22.1
(0.1) 		8.2
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 17.1
(-2.1)
P 		33.7
(3)
P 		14.3
(-3)
P 		19.3
(-1.5)
P 		33.7
(3)
P 		25.3
(0.4)
P 		19
B 31
(-1.3)
P 		31.8
(-1)
P 		26.7
(-2.8)
P 		26.2
(-3)
P 		42.7
(3)
P 		32.5
(-0.7)
P 		17
C 44.5
(3)
P 		24.6
(-0.6)
P 		23.5
(-0.8)
P 		11.3
(-3)
P 		26.8
(-0.2)
P 		27.5
(-0.1)
P 		33
avr 30.9
(1.6) 		30
(1.3) 		21.5
(-2) 		18.9
(-3) 		34.4
(3) 		28.4
(0.7) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177420_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3010
Sequence Type Exemplar sequence
Representative Public ID K09E4.2 NCBI
Target Description K09E4.2 /REP_DB=WormBase Gene ID /WP=CE25052 /TR=Q9NAP5 /GB=CAB70171.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064549(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064549 NCBI Caenorhabditis elegans K09E4.2 (K09E4.2) mRNA, complete cds. 11/11 None
GENEFINDER00000022868 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14127742:14132714:-1 11/11 None
K09E4.2 ENSEMBL cdna:known chromosome:CEL140:II:14127578:14132724:-1 gene:K09E4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14127738-14132711(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K09E4.2
Entrez Gene 175065 Entrez gene
SwissProt Q9NAP5 EMBL-EBI
WormBase CE32691 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15501_AT ALG3 family protein at
ATH1-121501:266467_AT ALG3 family protein at
CANINE:1601568_AT similar to alpha-1,3-mannosyltransferase ALG3 isoform c cfa
CANINE_2:CFAAFFX.19302.1.S1_AT similar to alpha-1,3-mannosyltransferase ALG3 isoform c cfa
DROSOPHILA_2:1641244_AT lethal (2) neighbor of tid dm
DROSGENOME1:141543_AT lethal (2) neighbor of tid dm
CHICKEN:GGAAFFX.5357.1.S1_AT similar to Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Dol-P-Man dependent alpha(1-3)-mannosyltransferase) (Not56-like protein) gga
CHICKEN:GGAAFFX.5357.2.S1_AT similar to Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Dol-P-Man dependent alpha(1-3)-mannosyltransferase) (Not56-like protein) gga
CHICKEN:GGAAFFX.5357.2.S1_S_AT similar to Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Dol-P-Man dependent alpha(1-3)-mannosyltransferase) (Not56-like protein) gga
HG-U133_PLUS_2:207396_S_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
HG-U133A:207396_S_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
HG-FOCUS:207396_S_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
HG-U133A_2:207396_S_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
HUGENEFL:Y09022_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
U133_X3P:G5031952_3P_A_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
HG-U95AV2:38161_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) hs
MG-U74AV2:103790_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MOE430A:1460742_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MOUSE430A_2:1460742_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MOUSE430_2:1460742_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MU11KSUBA:C78385_RC_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MU11KSUBA:C78385_RC_G_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) mm
MG-U74AV2:162337_F_AT Asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg3), mRNA mm
MU19KSUBA:TC20482_I_AT Asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg3), mRNA mm
MU19KSUBA:TC20960_AT Asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg3), mRNA mm
RAE230B:1379521_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) (predicted) rn
RAT230_2:1379521_AT asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase) (predicted) rn
YEAST_2:1772654_AT Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5783 endoplasmic reticulum inferred from electronic annotation QuickGO AmiGO
5874 microtubule inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
30 mannosyltransferase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB70171 Hypothetical protein K09E4.2 [Caenorhabditis elegans] ref|NP_496950.2| K09E4.2 [Caenorhabditis elegans] 0.0
blast CAE73534 Hypothetical protein CBG20997 [Caenorhabditis briggsae] 0.0
blast CAE73534 Hypothetical protein CBG20997 [Caenorhabditis briggsae] 1.0E-160
blast AAH95802 Hypothetical protein LOC553725 [Danio rerio] ref|NP_001018532.1| hypothetical protein LOC553725 [Danio rerio] 6.0E-91
blast XP_871059 PREDICTED: similar to Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Dol-P-Man dependent alpha(1-3)-mannosyltransferase) (Not56-like protein) isoform 1 [Bos taurus] 9.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR007873 EMBL-EBI ALG3 1.0E-126
Pfam IPR007873 EMBL-EBI ALG3 4.4E-63
Pfam IPR007873 EMBL-EBI ALG3 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496950.2 8 20-42,63-85,100-122,143-165,180-202,209-231,273-295,304-326
K09E4.2 8 20-42,63-85,100-122,143-165,180-202,209-231,273-295,304-326
GENEFINDER00000022868 4 20-42,108-130,140-162,207-226
Sequence
Target Sequence 
>C. ELEGANS:177420_S_AT
cacgtcatcattttctggcatctaatcgtgacgagacccgcgcgacctggcgattcagtg
tccagggagacgctccgaaggcttgagcagcggcagaaaatgcttgagcaagagctccga
gagcatggacaagttgtgccgaaattcaaatttccagcgtttttggaagagcagcaggaa
gagcaagtggatgttcggaagaggatgaagcagccgattggggagaagaggacgaaaaag
cagttggatgcggaatttgagacgtatttcgaggagcagatggacggatttcgggaggag
cacacgatttatctacaagctcaattaaatcccaatgaaactccaacaacatggaaatca
ttcaaatttcccaaaaatgccgacaaaaacctgctggaaagctatctgaagcttcacgga
gcaggagcacaacaagactctgaagaatctgaaatttcctcagccgaagaagaagcttca
gacgacgagaatagcggtgaggaagagcccaatacggagaaaattgagcaaaattcgtgt
aaagctgaagcctcggagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGTCATCATTTTCTGGCATCTAA 569 199 1225 Antisense
CGCGACCTGGCGATTCAGTGTCCAG 384 255 1265 Antisense
GATTCAGTGTCCAGGGAGACGCTCC 154 425 1276 Antisense
GAGACGCTCCGAAGGCTTGAGCAGC 7 395 1291 Antisense
GGACGGATTTCGGGAGGAGCACACG 177 525 1506 Antisense
AAATCCCAATGAAACTCCAACAACA 202 119 1551 Antisense
AAATGCCGACAAAAACCTGCTGGAA 631 117 1599 Antisense
GAAGCTTCACGGAGCAGGAGCACAA 579 341 1632 Antisense
GAATCTGAAATTTCCTCAGCCGAAG 562 327 1669 Antisense
GCGGTGAGGAAGAGCCCAATACGGA 635 289 1718 Antisense
GTGTAAAGCTGAAGCCTCGGAGCAA 232 483 1761 Antisense
Sequence Source Affymetrix Proprietary Database