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[177417_at]

Description F15A4.2 /REP_DB=WormBase Gene ID /WP=CE15839 /TR=O17817 /GB=CAB02946.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 39      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 4.4
(3)
A 		1.6
(-3)
A 		3
(-0)
A 		2.1
(-1.9)
A 		3
B 4.6
(3)
A 		1.5
(-3)
A 		1.8
(-2.4)
A 		1.5
(-3)
A 		3
C 13.6
(3)
A 		2.4
(-2.4)
A 		1.2
(-3)
A 		4.9
(-1.2)
A 		12
avr 7.5
(3) 		1.8
(-3) 		2
(-2.8) 		2.8
(-1.9) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 4.6
(-2.7)
A 		1.9
(-3)
A 		4.4
(-2.7)
A 		1.5
(-3)
A 		1.9
(-3)
A 		64.6
(3)
A 		63
B 11
(-1.3)
A 		1.5
(-3)
A 		2.8
(-2.8)
A 		1.5
(-3)
A 		3.6
(-2.6)
A 		34.2
(3)
A 		33
C 3.6
(-2.8)
A 		1.8
(-3)
A 		3.1
(-2.8)
A 		3.2
(-2.8)
A 		1.4
(-3)
A 		57.8
(3)
A 		56
avr 6.4
(-2.4) 		1.7
(-3) 		3.4
(-2.8) 		2.1
(-3) 		2.3
(-2.9) 		52.2
(3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1.6
(3)
A 		0.3
(-3)
A 		0.8
(-0.7)
A 		0.3
(-3)
A 		1
B 1.5
(3)
A 		0.4
(-2.5)
A 		0.4
(-2.5)
A 		0.3
(-3)
A 		1
C 4.5
(3)
A 		0.7
(-2.6)
A 		0.4
(-3)
A 		0.8
(-2.4)
A 		4
avr 2.5
(3) 		0.5
(-3) 		0.5
(-3) 		0.5
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 0.8
(-2.8)
A 		0.7
(-2.9)
A 		1
(-2.8)
A 		0.4
(-3)
A 		0.6
(-2.9)
A 		15.7
(3)
A 		15
B 3.8
(-1.3)
A 		0.5
(-3)
A 		0.7
(-2.9)
A 		0.4
(-3)
A 		1.5
(-2.5)
A 		12.7
(3)
A 		12
C 1.3
(-2.7)
A 		0.5
(-3)
A 		0.8
(-2.9)
A 		0.6
(-2.9)
A 		0.4
(-3)
A 		19.3
(3)
A 		19
avr 2
(-2.4) 		0.6
(-3) 		0.8
(-2.9) 		0.5
(-3) 		0.8
(-2.9) 		15.9
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177417_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3270
Sequence Type Exemplar sequence
Representative Public ID F15A4.2 NCBI
Target Description F15A4.2 /REP_DB=WormBase Gene ID /WP=CE15839 /TR=O17817 /GB=CAB02946.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064253(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064253 NCBI Caenorhabditis elegans F15A4.2 (F15A4.2) mRNA, complete cds. 11/11 None
SNAP00000042546 ENSEMBL cdna:SNAP chromosome:CEL140:II:12461031:12467422:1 10/11 None
GENEFINDER00000042560 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12462458:12463907:1 11/11 None
F15A4.2 ENSEMBL cdna:known chromosome:CEL140:II:12462458:12463907:1 gene:F15A4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12462454-12463904(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F15A4.2
Entrez Gene 184511 Entrez gene
SwissProt O17817 EMBL-EBI
WormBase CE15839 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02946 Hypothetical protein F15A4.2 [Caenorhabditis elegans] ref|NP_496654.1| F15A4.2 [Caenorhabditis elegans] 1.0E-140
blast CAB02946 Hypothetical protein F15A4.2 [Caenorhabditis elegans] ref|NP_496654.1| F15A4.2 [Caenorhabditis elegans] 1.0E-103
blast CAB60352 Hypothetical protein Y46G5A.7 [Caenorhabditis elegans] ref|NP_496713.2| Y46G5A.7 [Caenorhabditis elegans] 1.0E-43
blast CAI79250 Hypothetical protein Y46G5A.39 [Caenorhabditis elegans] ref|NP_001022443.1| Y46G5A.39 [Caenorhabditis elegans] 9.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 6.6E-8
Sequence
Target Sequence 
>C. ELEGANS:177417_AT
gagcggtgcacgagatggaactgtagcattgtacgctggatatgatattaatcgagtttt
ggatataacgtcggactttttcgactaccttgatatatacatatacctttatataatcga
taatcacagtgataaagatgctaagaaattagttgaagttatcgtaacaaaaggtatata
tgttcattatttgaggtttgacggagaacaaatctcacatgagctttttcgagaaatgct
taaatgtgatgtgaatgagttgagaattcatctaagaacacagcatacggttgaattgtc
cgatattgatttgatggtcagctgtaagtgtgtggcactgaccaggattactatacccag
tgattatttgaacgtgctgctcaaaaaatggcaggcgaaccggaacttgacatttttctc
ggcgtcagggatggctttaactcttgataaatctattattcttgaaggcacgaattctga
acaagggtctgaagaatacaacttttacataaaatgcaacaataggaagactgctctttt
gaaaattctccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGGTGCACGAGATGGAACTGTA 506 385 240 Antisense
GGAACTGTAGCATTGTACGCTGGAT 56 531 256 Antisense
CGGACTTTTTCGACTACCTTGATAT 323 239 311 Antisense
GATGGTCAGCTGTAAGTGTGTGGCA 574 407 552 Antisense
GTGTGTGGCACTGACCAGGATTACT 472 483 567 Antisense
GACCAGGATTACTATACCCAGTGAT 487 383 579 Antisense
AGTGATTATTTGAACGTGCTGCTCA 20 61 598 Antisense
AATGGCAGGCGAACCGGAACTTGAC 98 163 626 Antisense
GGAACTTGACATTTTTCTCGGCGTC 339 529 641 Antisense
TCGGCGTCAGGGATGGCTTTAACTC 675 603 658 Antisense
GACTGCTCTTTTGAAAATTCTCCAT 341 375 768 Antisense
Sequence Source Affymetrix Proprietary Database