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[177415_s_at]

Description F35E2.1 /REP_DB=WormBase Gene ID /WP=CE17771 /TR=O62224 /GB=CAB04282.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 780
1 2 3 4 range
A 48.7
(-2.7)
A 		8.5
(-3)
A 		44.2
(-2.7)
A 		788.1
(3)
P 		780
B 83.2
(-2.4)
A 		75.7
(-2.4)
A 		25.4
(-3)
A 		567.3
(3)
P 		542
C 103.4
(-2.3)
P 		59.7
(-2.7)
A 		32.5
(-3)
A 		648.6
(3)
P 		616
avr 78.4
(-2.6) 		48
(-2.9) 		34
(-3) 		668
(3) 		634

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 38.6
(-1.9)
A 		8.2
(-3)
A 		86.8
(-0.2)
P 		80.5
(-0.4)
M 		174.1
(3)
P 		139.9
(1.8)
P 		166
B 39.7
(-3)
A 		55.3
(-2.2)
A 		50.5
(-2.5)
A 		49.2
(-2.5)
A 		154.2
(2.6)
A 		163
(3)
P 		123
C 3.9
(-3)
A 		73.4
(-0.2)
A 		50
(-1.2)
A 		8
(-2.8)
A 		91.2
(0.5)
P 		154.5
(3)
P 		151
avr 27.4
(-3) 		45.6
(-2.1) 		62.4
(-1.3) 		45.9
(-2.1) 		139.8
(2.4) 		152.5
(3) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 17.7
(-2.2)
A 		1.8
(-3)
A 		11.6
(-2.5)
A 		115.4
(3)
P 		114
B 26.5
(-1.9)
A 		18.7
(-2.3)
A 		5.9
(-3)
A 		120.9
(3)
P 		115
C 34.2
(-1.6)
P 		17.2
(-2.6)
A 		11.3
(-3)
A 		109.4
(3)
P 		98
avr 26.1
(-2.1) 		12.6
(-2.8) 		9.6
(-3) 		115.2
(3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 7.1
(-2.5)
A 		2.9
(-3)
A 		18.9
(-1.1)
P 		20.4
(-0.9)
M 		52.5
(3)
P 		34.1
(0.8)
P 		50
B 13.8
(-2.8)
A 		18.4
(-2.3)
A 		12.3
(-3)
A 		13
(-2.9)
A 		62.6
(3)
A 		60.8
(2.8)
P 		50
C 1.4
(-3)
A 		22.4
(-0.5)
A 		12.7
(-1.7)
A 		1.6
(-3)
A 		29.5
(0.4)
P 		51.7
(3)
P 		50
avr 7.4
(-3) 		14.6
(-2) 		14.6
(-2) 		11.7
(-2.4) 		48.2
(2.9) 		48.9
(3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177415_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.736
Sequence Type Exemplar sequence
Representative Public ID F35E2.1 NCBI
Target Description F35E2.1 /REP_DB=WormBase Gene ID /WP=CE17771 /TR=O62224 /GB=CAB04282.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059871(11), NM_067936(11), NM_060590(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059871 NCBI Caenorhabditis elegans F16D3.6 (F16D3.6) mRNA, complete cds. 11/11 A 
NM_067936 NCBI Caenorhabditis elegans B0546.5 (B0546.5) mRNA, complete cds. 11/11 A 
NM_060590 NCBI Caenorhabditis elegans F35E2.1 (F35E2.1) mRNA, complete cds. 11/11 None
GENEFINDER00000041145 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11746848:11748884:-1 11/11 None
GENEFINDER00000036932 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8380875:8385660:1 11/11 None
SNAP00000036929 ENSEMBL cdna:SNAP chromosome:CEL140:I:8382862:8385660:1 9/11 None
F16D3.6 ENSEMBL cdna:known chromosome:CEL140:I:8383615:8385660:1 gene:F16D3.6 11/11 None
F35E2.1 ENSEMBL cdna:known chromosome:CEL140:I:11746848:11748884:-1 gene:F35E2.1 11/11 None
B0546.5 ENSEMBL cdna:known chromosome:CEL140:IV:3388073:3390084:-1 gene:B0546.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041138 2/11 Cross Hyb Matching Probes None
SNAP00000001412 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8383545-8385591(+) 98.36 98.36
chrI:11746778-11748815(-) 100.0 100.0
chrIV:3388052-3390090(-) 98.36 98.36
Public Domain and Genome References
Gene Title


Ensembl F16D3.6
F35E2.1
B0546.5
Entrez Gene 182031 Entrez gene
184571 Entrez gene
185284 Entrez gene
SwissProt O44442 EMBL-EBI
Q19494 EMBL-EBI
O62224 EMBL-EBI
WormBase CE34640 Wormbase
CE36133 Wormbase
CE17771 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01498 Hypothetical protein F16D3.6 [Caenorhabditis elegans] ref|NP_492272.2| F16D3.6 [Caenorhabditis elegans] 0.0
blast CAB04282 Hypothetical protein F35E2.1 [Caenorhabditis elegans] ref|NP_492991.1| F35E2.1 [Caenorhabditis elegans] 0.0
blast T19600 probable transposition protein C31A11.2 - Caenorhabditis elegans 0.0
blast T19555 hypothetical protein C29E6.6 - Caenorhabditis elegans 0.0
blast AAF60591 Hypothetical protein Y45G12C.13 [Caenorhabditis elegans] 0.0
blast CAB04282 Hypothetical protein F35E2.1 [Caenorhabditis elegans] ref|NP_492991.1| F35E2.1 [Caenorhabditis elegans] 1.0E-178
blast CAB01498 Hypothetical protein F16D3.6 [Caenorhabditis elegans] ref|NP_492272.2| F16D3.6 [Caenorhabditis elegans] 1.0E-178
Protein Domains
Database ID Description E-Value
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 2.0E-45
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR001888 EMBL-EBI Transposase, type 1 2.8E-41
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.0E-126
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 6.1E-61
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 1.2E-19
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 2.4E-42
Pfam IPR010601 EMBL-EBI Protein of unknown function DUF1182 6.4E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492272.2 7 44-66,79-101,111-133,154-176,196-218,239-261,271-293
NP_500337.2 4 44-66,79-101,155-177,198-220
NP_492991.1 7 44-66,79-101,111-133,154-176,200-222,243-265,275-297
F16D3.6 7 44-66,79-101,111-133,154-176,196-218,239-261,271-293
F35E2.1 7 44-66,79-101,111-133,154-176,200-222,243-265,275-297
B0546.5 4 44-66,79-101,155-177,198-220
GENEFINDER00000041145 7 44-66,79-101,111-133,154-176,200-222,243-265,275-297
GENEFINDER00000036932 7 383-405,418-440,450-472,493-512,538-560,581-603,613-635
SNAP00000036929 4 72-91,119-141,162-184,199-221
Sequence
Target Sequence 
>C. ELEGANS:177415_S_AT
gcggtatcattatatcgttatcgtatagttgttttgaaaggacatccatctccatggttt
gttataaatgttcacatcatagttacgacaatatttattatttacgcgtttctcaattat
ccatttggggaaaacaataagaatgatgtgtgttatacacttcgattcagtaatggaatg
gaacttgttcggatcttctcaacgttattttttaattttgcagccatcattgtcaatttt
gtaattcttcgatttgtgaaacactttgaaaatgagaataaaaatatccaatcaaaaata
attcaattaactcaaagtcttcttattcaatcagtgataccagtcattgtatctttgcct
ttattacttttatcatttgagttctattttggaatcccaatgccaagctcatttggatcc
acatggtatgctctaacattccttgggccatttctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGGTATCATTATATCGTTATCGTA 122 293 421 Antisense
AGGACATCCATCTCCATGGTTTGTT 608 55 459 Antisense
TTTACGCGTTTCTCAATTATCCATT 546 671 521 Antisense
GTGTGTTATACACTTCGATTCAGTA 667 487 568 Antisense
GAACTTGTTCGGATCTTCTCAACGT 181 349 601 Antisense
TTCGGATCTTCTCAACGTTATTTTT 179 695 608 Antisense
ATTTTGCAGCCATCATTGTCAATTT 508 13 635 Antisense
ACCAGTCATTGTATCTTTGCCTTTA 83 87 759 Antisense
TTGAGTTCTATTTTGGAATCCCAAT 539 703 797 Antisense
GGATCCACATGGTATGCTCTAACAT 64 513 835 Antisense
TAACATTCCTTGGGCCATTTCTCAT 401 639 854 Antisense
Sequence Source Affymetrix Proprietary Database