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[177414_at]

Description F58D5.5 /REP_DB=WormBase Gene ID /WP=CE25022 /TR=Q9NLD3 /GB=CAB70236.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 06      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 126.2
(3)
A 		63.6
(-3)
A 		115.1
(1.9)
A 		105.3
(1)
A 		63
B 146.9
(-0.1)
A 		59.2
(-3)
A 		131.9
(-0.6)
A 		241
(3)
A 		182
C 171.2
(3)
A 		126.9
(0.8)
A 		51.3
(-3)
A 		144.5
(1.7)
A 		120
avr 148.1
(1.8) 		83.2
(-3) 		99.4
(-1.8) 		163.6
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 59
(-1)
A 		66
(-0.1)
A 		44.3
(-3)
A 		56.6
(-1.3)
A 		50.9
(-2.1)
A 		89
(3)
A 		45
B 48.5
(2.2)
A 		47
(1.9)
A 		41.8
(1.1)
A 		15
(-3)
A 		48.9
(2.2)
A 		53.8
(3)
A 		39
C 98.8
(3)
A 		75.7
(1.4)
A 		10
(-3)
A 		32.7
(-1.5)
A 		32.8
(-1.5)
A 		79
(1.7)
A 		89
avr 68.8
(2.3) 		62.9
(1.4) 		32
(-3) 		34.8
(-2.6) 		44.2
(-1.3) 		73.9
(3) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 46
(3)
A 		13.8
(-3)
A 		30.1
(0)
A 		15.4
(-2.7)
A 		32
B 46.9
(2.3)
A 		14.6
(-3)
A 		30.6
(-0.4)
A 		51.4
(3)
A 		37
C 56.7
(3)
A 		36.5
(-0.1)
A 		17.9
(-3)
A 		24.4
(-2)
A 		39
avr 49.9
(3) 		21.6
(-3) 		26.2
(-2) 		30.4
(-1.1) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 10.9
(-2.5)
A 		23.5
(3)
A 		9.7
(-3)
A 		14.4
(-1)
A 		15.3
(-0.6)
A 		21.7
(2.2)
A 		14
B 16.9
(1.8)
A 		15.6
(1.3)
A 		10.2
(-0.7)
A 		4
(-3)
A 		19.9
(2.9)
A 		20.1
(3)
A 		16
C 36.6
(3)
A 		23.1
(0.6)
A 		2.5
(-3)
A 		6.6
(-2.3)
A 		10.6
(-1.6)
A 		26.5
(1.2)
A 		34
avr 21.5
(2.5) 		20.7
(2.2) 		7.5
(-3) 		8.3
(-2.7) 		15.3
(0.1) 		22.8
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177414_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.717
Sequence Type Exemplar sequence
Representative Public ID F58D5.5 NCBI
Target Description F58D5.5 /REP_DB=WormBase Gene ID /WP=CE25022 /TR=Q9NLD3 /GB=CAB70236.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060645(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060645 NCBI Caenorhabditis elegans F58D5.5 (F58D5.5) mRNA, complete cds. 10/11 None
SNAP00000034698 ENSEMBL cdna:SNAP chromosome:CEL140:I:12049280:12065294:1 9/11 None
GENEFINDER00000034709 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12049280:12051108:1 11/11 None
F58D5.5 ENSEMBL cdna:known chromosome:CEL140:I:12049157:12051108:1 gene:F58D5.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12049210-12051039(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58D5.5
Entrez Gene 186499 Entrez gene
SwissProt Q9NLD3 EMBL-EBI
WormBase CE30547 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70236 Hypothetical protein F58D5.5 [Caenorhabditis elegans] ref|NP_493046.2| F58D5.5 [Caenorhabditis elegans] 1.0E-32
blast CAB70236 Hypothetical protein F58D5.5 [Caenorhabditis elegans] ref|NP_493046.2| F58D5.5 [Caenorhabditis elegans] 2.0E-27
blast CAB70236 Hypothetical protein F58D5.5 [Caenorhabditis elegans] ref|NP_493046.2| F58D5.5 [Caenorhabditis elegans] 9.0E-25
Sequence
Target Sequence 
>C. ELEGANS:177414_AT
atcaatattcatctttttctccgtcttctccatcgtcatccagccgtcccagtggtggtg
gtggccagtcatcccatcagacgacgccgaagtcatccggaaatccacgtcagcaacaag
cacagccacgtcatcgccaacgaaaggctcaatcatcgagcccagcgtcgttggcagctt
ttgcatcatcatcggtgtgctcatcgccatccgccagaaatgtaccgttgccaccagttg
aatggcttaaccaggtacattggctgaaaaacaacaaaaaactcacttttttgtgtaaaa
ttttcaattttttgtcgaaaatcgcttttacagcaggaaaatcaccaaatctccaagtgc
ccggacagctcgaaaactcatcccgtccatcatcatccatctcaatgacgtcatcagctt
catcgtcaacatcttctctctcatcatcggatgctccaatgatcacaatgacaccacgac
gtgctgctcaaaaagagatgaagccaaagactgatgtgctcggagttcgtgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAATATTCATCTTTTTCTCCGTC 70 27 17 Antisense
TCAGACGACGCCGAAGTCATCCGGA 351 623 93 Antisense
GTCATCCGGAAATCCACGTCAGCAA 282 467 108 Antisense
GTCGTTGGCAGCTTTTGCATCATCA 466 473 183 Antisense
GCATCATCATCGGTGTGCTCATCGC 87 309 199 Antisense
ATCCGCCAGAAATGTACCGTTGCCA 468 27 225 Antisense
AAATCACCAAATCTCCAAGTGCCCG 145 117 355 Antisense
CCCGGACAGCTCGAAAACTCATCCC 272 259 376 Antisense
TCTCTCTCATCATCGGATGCTCCAA 38 613 451 Antisense
ATGACACCACGACGTGCTGCTCAAA 70 45 484 Antisense
GACTGATGTGCTCGGAGTTCGTGTC 569 375 525 Antisense
Sequence Source Affymetrix Proprietary Database