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[177413_s_at]

Description F31E8.2 /REP_DB=WormBase Gene ID /WP=CE02711 /CHR=2 /FEA=Sanger Annotation /DEF=locus:snt-1 Synaptotagmin (ST.LOUIS)
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 13      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 41      6grp_total > 13      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 40      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 524
1 2 3 4 range
A 843.9
(3)
P 		757.8
(1.9)
P 		375.5
(-3)
P 		423.3
(-2.4)
P 		468
B 819.2
(3)
P 		500.6
(-1.3)
P 		378.2
(-3)
P 		473.5
(-1.7)
P 		441
C 855
(3)
P 		331.1
(-3)
P 		492.3
(-1.2)
P 		555.8
(-0.4)
P 		524
avr 839.4
(3) 		529.8
(-1.4) 		415.3
(-3) 		484.2
(-2) 		424

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 496
1 2 3 4 5 6 range
A 813
(3)
P 		703.8
(1.4)
P 		592.7
(-0.3)
P 		568.5
(-0.7)
P 		442.1
(-2.6)
P 		414.9
(-3)
P 		398
B 708.8
(1.6)
P 		808.6
(3)
P 		728.5
(1.9)
P 		532.7
(-0.9)
P 		523.7
(-1)
P 		379.3
(-3)
P 		429
C 838.6
(2.5)
P 		871
(2.9)
P 		538.3
(-1.4)
P 		875.7
(3)
P 		520.2
(-1.6)
P 		415.5
(-3)
P 		460
avr 786.8
(2.9) 		794.5
(3) 		619.8
(0.3) 		659
(0.9) 		495.3
(-1.6) 		403.2
(-3) 		391
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 307.3
(3)
P 		164.5
(-0.5)
P 		98.2
(-2.1)
P 		62
(-3)
P 		245
B 261.2
(3)
P 		123.6
(-1.8)
P 		87.6
(-3)
P 		100.9
(-2.5)
P 		174
C 283
(3)
P 		95.2
(-3)
P 		171.6
(-0.5)
P 		93.7
(-3)
P 		189
avr 283.8
(3) 		127.8
(-1.7) 		119.1
(-2) 		85.5
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 149.9
(-1)
P 		250.3
(3)
P 		129.1
(-1.9)
P 		144.2
(-1.3)
P 		133.4
(-1.7)
P 		101
(-3)
P 		149
B 246.6
(1.9)
P 		269.2
(3)
P 		176.9
(-1.3)
P 		141.1
(-3)
P 		212.8
(0.4)
P 		141.4
(-3)
P 		128
C 310.8
(3)
P 		265.8
(1.4)
P 		137
(-3)
P 		176.1
(-1.7)
P 		168.3
(-1.9)
P 		139.1
(-2.9)
P 		174
avr 235.8
(1.8) 		261.8
(3) 		147.7
(-2.1) 		153.8
(-1.8) 		171.5
(-1) 		127.2
(-3) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177413_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5090
Sequence Type Exemplar sequence
Representative Public ID F31E8.2 NCBI
Target Description F31E8.2 /REP_DB=WormBase Gene ID /WP=CE02711 /CHR=2 /FEA=Sanger Annotation /DEF=locus:snt-1 Synaptotagmin (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026958(11), NM_062993(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026958 NCBI Caenorhabditis elegans F31E8.2a (Synaptotagmin) mRNA, complete cds. 11/11 None
NM_062993 NCBI Caenorhabditis elegans F31E8.2b (Synaptotagmin) mRNA, complete cds. 9/11 A 
SNAP00000019079 ENSEMBL cdna:SNAP chromosome:CEL140:II:6814021:6815909:1 11/11 None
GENEFINDER00000019090 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6810898:6815909:1 11/11 None
F31E8.2a ENSEMBL cdna:known chromosome:CEL140:II:6810893:6816365:1 gene:F31E8.2 11/11 A 
F31E8.2b ENSEMBL cdna:known chromosome:CEL140:II:6810898:6815909:1 gene:F31E8.2 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6810892-6815904(+) 100.0 100.0
Public Domain and Genome References
Ensembl F31E8.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022129 F31E8.2a [Caenorhabditis elegans] gb|AAA98023.1| Synaptotagmin protein 1, isoform a [Caenorhabditis elegans] sp|P34693|SYT1_CAEEL Synaptotagmin-1 (Synaptotagmin I) gb|AAA28145.1| synaptotagmin I 0.0
blast CAE65975 Hypothetical protein CBG11160 [Caenorhabditis briggsae] 0.0
blast NP_495394 F31E8.2b [Caenorhabditis elegans] gb|AAK68354.1| Synaptotagmin protein 1, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 5.1E-40
Pfam IPR000008 EMBL-EBI C2 domain 1.1E-38
Pfam IPR000008 EMBL-EBI C2 domain 6.6E-37
Pfam IPR000008 EMBL-EBI C2 domain 5.1E-40
Pfam IPR000008 EMBL-EBI C2 domain 1.1E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022129.1 1 75-97
NP_495394.3 1 75-97
F31E8.2a 1 75-97
F31E8.2b 1 75-97
GENEFINDER00000019090 1 75-97
Sequence
Target Sequence 
>C. ELEGANS:177413_S_AT
atcggacaagttctcattccgcttggaaaaattgatttgggagctgttatcgaagaatgg
aaggatattgcaccaccaccagatgacaaagaagctgagaagagtcttggtgacatttgc
ttctcacttcggtacgtcccaactgctggtaaattgacagtggtcattctggaagcaaaa
aatcttaagaaaatggacgtcggtggtttatcagatccttatgtgaagattgtgttgatg
caaggtggaaaacgactgaaaaagaagaagacatcaatcaaaaagtgtacacttaaccca
tattataacgaatcgttcagctttgaagtgcctttcgaacaaattcagaaagtttccctt
atgatcactgtgatggattatgataaacttggatccaatgacgctattggaaggtgtcta
ttgggatgtaatggaaccggtgccgagctgaggcattggatggatatgttggcttcacca
cgtcgtccaattgctcaatggcatacacttggaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGGACAAGTTCTCATTCCGCTTG 270 33 784 Antisense
AATGGAAGGATATTGCACCACCACC 139 163 839 Antisense
GAGTCTTGGTGACATTTGCTTCTCA 603 399 885 Antisense
GTACGTCCCAACTGCTGGTAAATTG 431 457 915 Antisense
GACGTCGGTGGTTTATCAGATCCTT 689 377 979 Antisense
GTTCAGCTTTGAAGTGCCTTTCGAA 338 445 1098 Antisense
GAAAGTTTCCCTTATGATCACTGTG 690 359 1131 Antisense
GATGTAATGGAACCGGTGCCGAGCT 318 409 1208 Antisense
ATGGATATGTTGGCTTCACCACGTC 612 51 1243 Antisense
CCACGTCGTCCAATTGCTCAATGGC 159 275 1261 Antisense
GCTCAATGGCATACACTTGGACCAG 383 301 1276 Antisense
Sequence Source Affymetrix Proprietary Database