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[177412_at]

Description C54G4.5 /REP_DB=WormBase Gene ID /WP=CE23613 /TR=Q18850 /GB=CAA99817.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 70      6grp_total > 17      4grp_total > 37      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 541.8
(3)
P 		538.4
(2.9)
P 		199.9
(-3)
P 		488
(2.1)
P 		342
B 551.4
(3)
P 		501.5
(1.9)
P 		281.8
(-3)
P 		413
(-0.1)
P 		270
C 579.9
(3)
P 		561
(2.6)
P 		312.9
(-3)
P 		477.8
(0.7)
P 		267
avr 557.7
(3) 		533.6
(2.5) 		264.9
(-3) 		459.6
(1) 		293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 412
1 2 3 4 5 6 range
A 595.4
(3)
P 		292.3
(-1.4)
P 		228.9
(-2.3)
P 		183.6
(-3)
P 		265.4
(-1.8)
P 		260.2
(-1.9)
P 		412
B 584.8
(3)
P 		330.6
(-0.9)
P 		303.5
(-1.3)
P 		193.5
(-3)
P 		277.5
(-1.7)
P 		276.1
(-1.7)
P 		391
C 539.6
(3)
P 		320
(-0.9)
P 		203.2
(-3)
P 		222.7
(-2.7)
P 		273.1
(-1.8)
P 		295.1
(-1.4)
P 		336
avr 573.3
(3) 		314.3
(-1.2) 		245.2
(-2.3) 		199.9
(-3) 		272
(-1.8) 		277.1
(-1.8) 		373
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 197.3
(3)
P 		116.9
(-0.3)
P 		52.3
(-3)
P 		71.4
(-2.2)
P 		145
B 175.8
(3)
P 		123.9
(0.2)
P 		65.3
(-3)
P 		88
(-1.8)
P 		111
C 192
(3)
P 		161.3
(1.3)
P 		109.1
(-1.5)
P 		80.6
(-3)
P 		111
avr 188.4
(3) 		134
(0.1) 		75.6
(-3) 		80
(-2.8) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 109.8
(3)
P 		103.9
(2.4)
P 		49.9
(-2.7)
P 		46.6
(-3)
P 		80.1
(0.2)
P 		63.3
(-1.4)
P 		63
B 203.5
(3)
P 		110.1
(-0.7)
P 		73.7
(-2.1)
P 		51.2
(-3)
P 		112.8
(-0.6)
P 		103
(-1)
P 		152
C 200
(3)
P 		97.6
(-1)
P 		51.7
(-2.7)
P 		44.8
(-3)
P 		88.4
(-1.3)
P 		98.8
(-0.9)
P 		155
avr 171.1
(3) 		103.9
(-0.3) 		58.4
(-2.5) 		47.5
(-3) 		93.8
(-0.8) 		88.4
(-1) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177412_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1793
Sequence Type Exemplar sequence
Representative Public ID C54G4.5 NCBI
Target Description C54G4.5 /REP_DB=WormBase Gene ID /WP=CE23613 /TR=Q18850 /GB=CAA99817.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059799(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059799 NCBI Caenorhabditis elegans C54G4.5 (C54G4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000028908 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8003902:8004710:1 11/11 None
C54G4.5 ENSEMBL cdna:known chromosome:CEL140:I:8003883:8004795:1 gene:C54G4.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028895 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8003832-8004641(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54G4.5
Entrez Gene 172578 Entrez gene
SwissProt Q18850 EMBL-EBI
WormBase CE23613 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99817 Hypothetical protein C54G4.5 [Caenorhabditis elegans] ref|NP_492200.1| C54G4.5 [Caenorhabditis elegans] 2.0E-63
blast CAE63714 Hypothetical protein CBG08235 [Caenorhabditis briggsae] 4.0E-56
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492200.1 1 20-42
C54G4.5 1 20-42
GENEFINDER00000028908 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:177412_AT
gaccaccttcatcatttactacgtcaacttctgcaaaacctgatgttgcatggtcatctt
ttttaatggtttactcaattccgtttatattggttctcacagtggcgtgcattataaagt
ggcgaccaatgagggaatttccaaatttgagaagttttttcagctggtactgctcatgct
ctcctaaaaaagtgatacaagaacttgaacaacctgatcgtacacatatgataatgtcaa
ttgacgcacagagtcttgcatcaatgtcttcagttattgattcaccacttcttgtccgaa
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCACCTTCATCATTTACTACGTC 619 381 26 Antisense
TTTACTACGTCAACTTCTGCAAAAC 571 671 40 Antisense
ATGGTTTACTCAATTCCGTTTATAT 476 51 91 Antisense
ATTGGTTCTCACAGTGGCGTGCATT 704 3 114 Antisense
GGCGTGCATTATAAAGTGGCGACCA 489 539 129 Antisense
GAAGTTTTTTCAGCTGGTACTGCTC 431 337 176 Antisense
TACTGCTCATGCTCTCCTAAAAAAG 611 645 193 Antisense
GAACTTGAACAACCTGATCGTACAC 593 349 226 Antisense
GTCAATTGACGCACAGAGTCTTGCA 256 461 261 Antisense
GAGTCTTGCATCAATGTCTTCAGTT 76 401 276 Antisense
TTGATTCACCACTTCTTGTCCGAAG 30 705 302 Antisense
Sequence Source Affymetrix Proprietary Database