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[177402_s_at]

Description D2030.6 /REP_DB=WormBase Gene ID /WP=CE09083 /TR=P90786 /GB=CAA98113.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 79      6grp_total > 96      4grp_total > 59      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1328
1 2 3 4 range
A 1319.1
(1)
P 		1529.4
(2)
P 		1731.3
(3)
P 		495.2
(-3)
P 		1236
B 1110
(0.3)
P 		1670.1
(3)
P 		1358.2
(1.5)
P 		443.4
(-3)
P 		1227
C 1181
(0.9)
P 		1612.4
(3)
P 		1572.8
(2.8)
P 		403.5
(-3)
P 		1209
avr 1203.4
(0.9) 		1604
(3) 		1554.1
(2.7) 		447.4
(-3) 		1157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1846
1 2 3 4 5 6 range
A 692.2
(-3)
P 		1373.1
(-0.8)
P 		867.5
(-2.4)
P 		1573.3
(-0.1)
P 		1902.3
(0.9)
P 		2538.4
(3)
P 		1846
B 707.6
(-3)
P 		1243.7
(-1.1)
P 		1080.6
(-1.7)
P 		1520.3
(-0.1)
P 		1845.1
(1.1)
P 		2379.7
(3)
P 		1672
C 808.1
(-3)
P 		1206.5
(-1.5)
P 		1431.3
(-0.6)
P 		1503.6
(-0.3)
P 		2290.7
(2.7)
P 		2355.9
(3)
P 		1548
avr 736
(-3) 		1274.4
(-1.1) 		1126.5
(-1.6) 		1532.4
(-0.2) 		2012.7
(1.5) 		2424.7
(3) 		1689
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 480.3
(3)
P 		332
(0.8)
P 		452.6
(2.6)
P 		72.5
(-3)
P 		408
B 354
(1.9)
P 		412.5
(3)
P 		314.7
(1.2)
P 		94.5
(-3)
P 		318
C 390.9
(1)
P 		463.6
(1.9)
P 		548.2
(3)
P 		68
(-3)
P 		480
avr 408.4
(2.5) 		402.7
(2.4) 		438.5
(3) 		78.3
(-3) 		360

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 760
1 2 3 4 5 6 range
A 127.6
(-3)
P 		488.4
(1.4)
P 		188.9
(-2.3)
P 		399
(0.3)
P 		574
(2.5)
P 		618
(3)
P 		490
B 246.2
(-3)
P 		414
(-1.4)
P 		262.4
(-2.8)
P 		402.7
(-1.5)
P 		749.6
(1.7)
P 		887.4
(3)
P 		641
C 299.5
(-3)
P 		368.2
(-2.2)
P 		364.3
(-2.2)
P 		302.4
(-3)
P 		741.2
(2.4)
P 		788.6
(3)
P 		489
avr 224.4
(-3) 		423.5
(-0.8) 		271.9
(-2.5) 		368
(-1.4) 		688.3
(2.2) 		764.7
(3) 		540
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177402_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.636
Sequence Type Exemplar sequence
Representative Public ID D2030.6 NCBI
Target Description D2030.6 /REP_DB=WormBase Gene ID /WP=CE09083 /TR=P90786 /GB=CAA98113.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059720(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059720 NCBI Caenorhabditis elegans D2030.6 (D2030.6) mRNA, complete cds. 11/11 None
SNAP00000031276 ENSEMBL cdna:SNAP chromosome:CEL140:I:7593641:7596488:-1 11/11 None
GENEFINDER00000031285 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7593641:7596488:-1 11/11 None
D2030.6 ENSEMBL cdna:known chromosome:CEL140:I:7593462:7596494:-1 gene:D2030.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7593571-7596419(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl D2030.6
Entrez Gene 172515 Entrez gene
SwissProt P90786 EMBL-EBI
WormBase CE09083 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.11107.1.S1_S_AT similar to piwi like homolog 1 cfa
CHICKEN:GGA.16962.1.S1_AT piwi-like 1 (Drosophila) gga
CHICKEN:GGAAFFX.25561.1.S1_S_AT piwi-like 1 (Drosophila) gga
HG-U95AV2:35901_AT piwi-like 1 (Drosophila) hs
U133_X3P:HS.194712.0.S1_3P_X_AT piwi-like 1 (Drosophila) hs
U133_X3P:HS.194712.0.S1_3P_AT piwi-like 1 (Drosophila) hs
HG-U133A_2:214868_AT piwi-like 1 (Drosophila) hs
HG-U133_PLUS_2:214868_AT piwi-like 1 (Drosophila) hs
HG-U133A:214868_AT piwi-like 1 (Drosophila) hs
HG-U95D:76328_I_AT piwi-like 1 (Drosophila) hs
HG-U95D:76330_R_AT piwi-like 1 (Drosophila) hs
MOE430A:1460340_AT piwi like homolog 1 (Drosophila) mm
MOUSE430A_2:1460340_AT piwi like homolog 1 (Drosophila) mm
MOUSE430_2:1460340_AT piwi like homolog 1 (Drosophila) mm
Protein Similarities
Method ID Description E-Value
blast CAA98113 Hypothetical protein D2030.6 [Caenorhabditis elegans] ref|NP_492121.1| D2030.6 [Caenorhabditis elegans] 0.0
blast AAB37734 Temporarily assigned gene name protein 292 [Caenorhabditis elegans] ref|NP_500994.1| Temporarily Assigned Gene name family member (tag-292) [Caenorhabditis elegans] 0.0
blast CAE66621 Hypothetical protein CBG11957 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:177402_S_AT
cgatagcaacgaccaaactctgccgagccgcttgatcctctacagagatggtgctggcga
cggacagattccgtatatcaagaacaccgaagtcaaactggttcgagacgcgtgtgacgc
tgtgaccgacaaagctgcggaactgagcaacaaggttcaggaaaagatcaaactcgcatt
tatcattgtcacgaaacgcgtgaacatgcgtattctcaagcagggatcgtcgcttgataa
tgctatcaatcctcaaccgggtaccgttgttgacacaactgtcactcgtccggagagaat
ggacttctacttggttccacagttcgtcaaccagggaactgtaacacctgtatcctacaa
tatcattcacgatgatactgatcttgggccagacaagcatcaacagcttgcattcaagtt
gtgccacctctactacaactggcagggaacagttcgtgttcctgctccatgtcaatacgc
ccacaaactcgcattcttgacagcccaatctcttcacgatgatgccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATAGCAACGACCAAACTCTGCCG 324 249 1926 Antisense
GCCGCTTGATCCTCTACAGAGATGG 530 285 1952 Antisense
ATGGTGCTGGCGACGGACAGATTCC 379 51 1973 Antisense
AACTGGTTCGAGACGCGTGTGACGC 115 143 2021 Antisense
GTGTGACGCTGTGACCGACAAAGCT 104 489 2037 Antisense
GGGATCGTCGCTTGATAATGCTATC 678 495 2148 Antisense
TAATGCTATCAATCCTCAACCGGGT 80 637 2163 Antisense
GGGAACTGTAACACCTGTATCCTAC 668 401 2259 Antisense
TGCATTCAAGTTGTGCCACCTCTAC 190 579 2334 Antisense
CCCACAAACTCGCATTCTTGACAGC 25 259 2405 Antisense
CCCAATCTCTTCACGATGATGCCAA 671 257 2429 Antisense
Sequence Source Affymetrix Proprietary Database