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[177400_at]

Description T22H6.3 /REP_DB=WormBase Gene ID /WP=CE16441 /TR=O18114 /GB=CAA90674.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 0      6grp_cgc > 87      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 2.8
(-3)
A 		5.3
(-2.6)
A 		31.1
(1.4)
A 		41
(3)
A 		38
B 41.2
(3)
A 		7.6
(-3)
A 		34.9
(1.9)
A 		30.6
(1.1)
A 		34
C 16.4
(-0)
A 		17.9
(0.3)
A 		2.6
(-3)
A 		30.3
(3)
A 		28
avr 20.1
(-0.5) 		10.3
(-3) 		22.9
(0.2) 		34
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 32.6
(-1.3)
A 		2.8
(-3)
A 		54.4
(-0.1)
P 		88.5
(1.8)
A 		110.5
(3)
P 		36.1
(-1.1)
A 		108
B 47.2
(-1.2)
A 		30.3
(-2.7)
A 		26.9
(-3)
A 		59.8
(-0)
A 		93.5
(3)
P 		46.2
(-1.3)
A 		67
C 24.3
(-1.1)
A 		49.5
(1.5)
A 		6.2
(-3)
A 		37.1
(0.2)
A 		64.3
(3)
A 		21.1
(-1.5)
A 		58
avr 34.7
(-2.3) 		27.5
(-3) 		29.2
(-2.8) 		61.8
(0.3) 		89.4
(3) 		34.5
(-2.3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1
(-3)
A 		1.1
(-2.9)
A 		8.1
(3)
A 		6
(1.2)
A 		7
B 13.1
(3)
A 		1.9
(-3)
A 		8.1
(0.3)
A 		6.5
(-0.5)
A 		11
C 5.4
(3)
A 		5.2
(2.7)
A 		0.9
(-3)
A 		5.1
(2.6)
A 		5
avr 6.5
(3) 		2.7
(-3) 		5.7
(1.7) 		5.9
(2.1) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 6
(-2.1)
A 		1
(-3)
A 		11.8
(-1)
P 		22.4
(1)
A 		33.3
(3)
P 		8.8
(-1.6)
A 		32
B 16.4
(-1.1)
A 		10.1
(-2.3)
A 		6.5
(-3)
A 		15.8
(-1.2)
A 		38
(3)
P 		17.2
(-1)
A 		32
C 9
(-0.7)
A 		15.1
(1.2)
A 		1.6
(-3)
A 		7.5
(-1.2)
A 		20.8
(3)
A 		7.1
(-1.3)
A 		19
avr 10.5
(-2) 		8.7
(-2.5) 		6.6
(-3) 		15.2
(-0.9) 		30.7
(3) 		11
(-1.9) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177400_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17637
Sequence Type Exemplar sequence
Representative Public ID T22H6.3 NCBI
Target Description T22H6.3 /REP_DB=WormBase Gene ID /WP=CE16441 /TR=O18114 /GB=CAA90674.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077733(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077733 NCBI Caenorhabditis elegans Seven TM Receptor family member (str-124) (str-124) mRNA, complete cds. 11/11 None
GENEFINDER00000019115 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12787438:12789143:1 11/11 None
T22H6.3 ENSEMBL cdna:known chromosome:CEL140:X:12787580:12789175:1 gene:T22H6.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019108 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12787842-12789143(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol str-124
Ensembl T22H6.3
Entrez Gene 188768 Entrez gene
SwissProt O18114 EMBL-EBI
WormBase CE16441 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90674 Hypothetical protein T22H6.3 [Caenorhabditis elegans] ref|NP_510134.1| Seven TM Receptor family member (str-124) [Caenorhabditis elegans] 0.0
blast CAE57214 Hypothetical protein CBG00072 [Caenorhabditis briggsae] 1.0E-130
blast CAA90675 Hypothetical protein T22H6.4 [Caenorhabditis elegans] ref|NP_510135.1| Seven TM Receptor family member (str-123) [Caenorhabditis elegans] 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.0E-126
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510134.1 7 10-32,45-67,87-109,130-152,201-223,255-277,287-305
T22H6.3 7 10-32,45-67,87-109,130-152,201-223,255-277,287-305
GENEFINDER00000019115 7 43-65,78-100,120-142,164-186,235-257,289-311,321-339
Sequence
Target Sequence 
>C. ELEGANS:177400_AT
ccgtgctttctgattttcttcgtttggacattaaatgtatttctgtttatgaaaccagac
caagaaaaagaggattattactatgatgttttaatggatggctacggcatagactcccac
aaagtttcattcttatcaatgatgtataagtcaccagacactgataccacgccagcccat
tggaatctttttcaactttttggcctagcaatttgttgtgttattatgacgtcatgcttc
tcaataatcattttttgtggcttcaaatcttggaaacaaatgaaaaactgcacaactcaa
atgagcaagaaaactagagaacttaatcgccagcttttcttaacactcggaatacagacg
ctgctcccattgataacaatgtatctacctggcggatgtttcattcttctcccggctttt
ggaatagagcttggagctaatgccaaccaaactggagcgttcattggagtatatcctgct
ctagatccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTGCTTTCTGATTTTCTTCGTTT 489 265 418 Antisense
TGGATGGCTACGGCATAGACTCCCA 701 549 512 Antisense
TAGACTCCCACAAAGTTTCATTCTT 343 653 527 Antisense
AAGTCACCAGACACTGATACCACGC 617 155 565 Antisense
CACGCCAGCCCATTGGAATCTTTTT 284 201 585 Antisense
GAACTTAATCGCCAGCTTTTCTTAA 46 351 736 Antisense
AACACTCGGAATACAGACGCTGCTC 287 135 759 Antisense
GACGCTGCTCCCATTGATAACAATG 177 381 774 Antisense
CCTGGCGGATGTTTCATTCTTCTCC 589 265 805 Antisense
TTCTTCTCCCGGCTTTTGGAATAGA 245 677 821 Antisense
GGAGTATATCCTGCTCTAGATCCCT 36 517 883 Antisense
Sequence Source Affymetrix Proprietary Database