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[177398_at]

Description W01A8.4 /REP_DB=WormBase Gene ID /WP=CE06534 /TR=Q23098 /GB=CAA95851.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 25      6grp_total > 61      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4006
1 2 3 4 range
A 4111.6
(0.5)
P 		5672.2
(3)
P 		5357.7
(2.5)
P 		1932
(-3)
P 		3740
B 3313.9
(-0.3)
P 		4472.6
(1.7)
P 		5214.7
(3)
P 		1739.1
(-3)
P 		3476
C 4073.9
(1.6)
P 		4412.8
(2.2)
P 		4807.7
(3)
P 		1665.9
(-3)
P 		3142
avr 3833.1
(0.7) 		4852.5
(2.5) 		5126.7
(3) 		1779
(-3) 		3348

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3334
1 2 3 4 5 6 range
A 3483.6
(-1.6)
P 		3918.9
(-0.8)
P 		6028.2
(3)
P 		5180.7
(1.5)
P 		3718.3
(-1.2)
P 		2694.3
(-3)
P 		3334
B 3586.2
(-1.2)
P 		4277.1
(0.7)
P 		5124.5
(3)
P 		4554.9
(1.4)
P 		4311.8
(0.8)
P 		2938.4
(-3)
P 		2186
C 4024.9
(-0.8)
P 		4389.4
(0)
P 		5032.1
(1.5)
P 		5700
(3)
P 		3973.6
(-0.9)
P 		3036
(-3)
P 		2664
avr 3698.2
(-1.1) 		4195.1
(0.1) 		5394.9
(3) 		5145.2
(2.4) 		4001.2
(-0.3) 		2889.6
(-3) 		2505
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1395
1 2 3 4 range
A 1497
(3)
P 		1231.3
(1.7)
P 		1400.6
(2.5)
P 		282.8
(-3)
P 		1214
B 1056.8
(1.9)
P 		1104.6
(2.3)
P 		1208.4
(3)
P 		370.6
(-3)
P 		838
C 1348.6
(1.6)
P 		1268.7
(1.2)
P 		1675.6
(3)
P 		280.9
(-3)
P 		1395
avr 1300.8
(2.3) 		1201.5
(1.8) 		1428.2
(3) 		311.4
(-3) 		1117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1110
1 2 3 4 5 6 range
A 642.2
(-3)
P 		1393.7
(3)
P 		1312.8
(2.4)
P 		1313.9
(2.4)
P 		1122
(0.8)
P 		656
(-2.9)
P 		752
B 1247.8
(-1.6)
P 		1423.7
(-0)
P 		1244.4
(-1.6)
P 		1206.4
(-2)
P 		1751.8
(3)
P 		1095.7
(-3)
P 		656
C 1491.9
(3)
P 		1339.3
(1.1)
P 		1280.7
(0.3)
P 		1146.3
(-1.4)
P 		1285.8
(0.4)
P 		1016.3
(-3)
P 		476
avr 1127.3
(-0.4) 		1385.6
(3) 		1279.3
(1.6) 		1222.2
(0.9) 		1386.5
(3) 		922.7
(-3) 		464
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177398_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.449
Sequence Type Exemplar sequence
Representative Public ID W01A8.4 NCBI
Target Description W01A8.4 /REP_DB=WormBase Gene ID /WP=CE06534 /TR=Q23098 /GB=CAA95851.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059600(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059600 NCBI Caenorhabditis elegans W01A8.4 (W01A8.4) mRNA, complete cds. 11/11 None
SNAP00000018611 ENSEMBL cdna:SNAP chromosome:CEL140:I:7076368:7077307:-1 11/11 None
GENEFINDER00000018615 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7076368:7077307:-1 11/11 None
W01A8.4 ENSEMBL cdna:known chromosome:CEL140:I:7076244:7077320:-1 gene:W01A8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7076298-7076562(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W01A8.4
Entrez Gene 172438 Entrez gene
SwissProt Q23098 EMBL-EBI
WormBase CE32507 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA95851 Hypothetical protein W01A8.4 [Caenorhabditis elegans] ref|NP_492001.2| W01A8.4 [Caenorhabditis elegans] 2.0E-99
blast CAE65706 Hypothetical protein CBG10781 [Caenorhabditis briggsae] 2.0E-98
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492001.2 1 111-133
W01A8.4 1 111-133
SNAP00000018611 1 111-133
GENEFINDER00000018615 1 111-133
Sequence
Target Sequence 
>C. ELEGANS:177398_AT
atggatccagcaatgttccgttggtattctgctgatatgactcaagccgagttcttccgt
ttcacaccacgcaccgtattcctgtacgtcggaaccgttttcgctctcttctacatttac
actcgtcttatgtttgtcccaatggacaagtcgaatgaagcatgtttggatggaaagctt
ctttggtgggatcgtcaacaaggaagaactctacaaaccggaggatccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGATCCAGCAATGTTCCGTTGGT 8 53 13 Antisense
CAATGTTCCGTTGGTATTCTGCTGA 338 183 23 Antisense
GTTGGTATTCTGCTGATATGACTCA 391 437 32 Antisense
TATGACTCAAGCCGAGTTCTTCCGT 624 651 48 Antisense
TGTACGTCGGAACCGTTTTCGCTCT 445 559 95 Antisense
CTCTTCTACATTTACACTCGTCTTA 174 229 118 Antisense
TACACTCGTCTTATGTTTGTCCCAA 171 643 130 Antisense
TCGTCTTATGTTTGTCCCAATGGAC 201 589 135 Antisense
GGACAAGTCGAATGAAGCATGTTTG 218 527 156 Antisense
GAAAGCTTCTTTGGTGGGATCGTCA 597 359 185 Antisense
AGAACTCTACAAACCGGAGGATCCG 456 63 217 Antisense
Sequence Source Affymetrix Proprietary Database