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[177394_at]

Description M01F1.6 /REP_DB=WormBase Gene ID /WP=CE01034 /TR=Q21454 /GB=CAA86518.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 02      4grp_high_8_8 > 45      6grp_highest_nonorm > 67      6grp_total > 51      4grp_total > 50      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1428
1 2 3 4 range
A 1986.3
(-2.6)
P 		2781.4
(3)
P 		2450.8
(0.7)
P 		1936.4
(-3)
P 		845
B 1754.8
(-2.1)
P 		2572.5
(1.3)
P 		2969.3
(3)
P 		1540.9
(-3)
P 		1428
C 2141.3
(-3)
P 		2627.7
(3)
P 		2197
(-2.3)
P 		2219.7
(-2)
P 		486
avr 1960.8
(-2.5) 		2660.5
(3) 		2539
(2) 		1899
(-3) 		762

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1046
1 2 3 4 5 6 range
A 2142.2
(1.7)
P 		1528.2
(-1.9)
P 		2359.4
(3)
P 		1537
(-1.8)
P 		1668.5
(-1.1)
P 		1340.6
(-3)
P 		1019
B 1580.9
(-2.5)
P 		1848.6
(-0.2)
P 		2080.9
(1.8)
P 		1964
(0.8)
P 		2227.6
(3)
P 		1521.4
(-3)
P 		706
C 1488.1
(-2.4)
P 		2032.1
(0.9)
P 		1529.4
(-2.1)
P 		2387
(3)
P 		1383.4
(-3)
P 		1607.6
(-1.7)
P 		1004
avr 1737.1
(-0) 		1803
(0.8) 		1989.9
(3) 		1962.7
(2.7) 		1759.8
(0.2) 		1489.9
(-3) 		500
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 482
1 2 3 4 range
A 723.2
(3)
P 		603.8
(1.4)
P 		640.7
(1.9)
P 		283.5
(-3)
P 		440
B 559.6
(0.9)
P 		635.4
(2.1)
P 		688.1
(3)
P 		328.4
(-3)
P 		360
C 708.8
(2.1)
P 		755.5
(2.8)
P 		765.7
(3)
P 		374.3
(-3)
P 		391
avr 663.9
(2.4) 		664.9
(2.5) 		698.2
(3) 		328.7
(-3) 		370

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 579
1 2 3 4 5 6 range
A 394.9
(-1.1)
P 		543.5
(3)
P 		513.8
(2.2)
P 		389.8
(-1.2)
P 		503.5
(1.9)
P 		326.4
(-3)
P 		217
B 550.1
(-2.3)
P 		615.3
(-1.3)
P 		505.3
(-3)
P 		520.2
(-2.8)
P 		905
(3)
P 		567.3
(-2.1)
P 		400
C 551.6
(1.2)
P 		620
(3)
P 		389.3
(-3)
P 		480
(-0.6)
P 		447.7
(-1.5)
P 		538.2
(0.9)
P 		231
avr 498.9
(-1.6) 		592.9
(2) 		469.5
(-2.8) 		463.3
(-3) 		618.7
(3) 		477.3
(-2.5) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177394_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7568
Sequence Type Exemplar sequence
Representative Public ID M01F1.6 NCBI
Target Description M01F1.6 /REP_DB=WormBase Gene ID /WP=CE01034 /TR=Q21454 /GB=CAA86518.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065323(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065323 NCBI Caenorhabditis elegans M01F1.6 (M01F1.6) mRNA, complete cds. 10/11 None
SNAP00000008223 ENSEMBL cdna:SNAP chromosome:CEL140:III:3490057:3513842:1 10/11 None
GENEFINDER00000008233 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3510228:3510904:1 10/11 None
M01F1.6.2 ENSEMBL cdna:known chromosome:CEL140:III:3510218:3510912:1 gene:M01F1.6 10/11 None
M01F1.6.1 ENSEMBL cdna:known chromosome:CEL140:III:3510220:3510932:1 gene:M01F1.6 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3510113-3510904(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M01F1.6
Entrez Gene 187375 Entrez gene
SwissProt Q21454 EMBL-EBI
WormBase CE37982 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86518 Hypothetical protein M01F1.6 [Caenorhabditis elegans] ref|NP_497724.2| M01F1.6 [Caenorhabditis elegans] sp|Q21454|RM35_CAEEL Probable 39S ribosomal protein L35, mitochondrial precursor (L35mt) 4.0E-92
blast CAA86518 Hypothetical protein M01F1.6 [Caenorhabditis elegans] ref|NP_497724.2| M01F1.6 [Caenorhabditis elegans] sp|Q21454|RM35_CAEEL Probable 39S ribosomal protein L35, mitochondrial precursor (L35mt) 2.0E-87
blast CAE64946 Hypothetical protein CBG09777 [Caenorhabditis briggsae] 4.0E-85
blast CAE64946 Hypothetical protein CBG09777 [Caenorhabditis briggsae] 1.0E-80
Sequence
Target Sequence 
>C. ELEGANS:177394_AT
atgctgaccgtagtcgctttgaaaattcaatttttattttgtatcaatttttttcctgat
tttttctcaatttcacatgaaatttacctttactttttattttcagccaaaaaaatgttg
ggaaaaacggcgttgagcacattatgtgcatcaagacaggctccgatcacattggccgcc
cgtggaatcgccaatattccggctcacgagtatcacattcgattcgatcagaaagtcgga
cggaaacggccggcgcaagatgttttagacagattcaaacgtctaaacaatggaatgtgg
attcacgcgcatccaggacgtcataagctgagatatatgaaagatgaaacgtggcagaag
actagtctttattatgagacctgtaccaaggaacagtgcgaaattctcgacaaactgatg
actccatattggctccgtccaaagcattatccaaatgatccatattctgcctataacgtc
cgccataacgtcacctcaccacgtgtcgatgatcgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTGACCGTAGTCGCTTTGAAAA 275 41 13 Antisense
AAGACAGGCTCCGATCACATTGGCC 471 153 165 Antisense
ATCGCCAATATTCCGGCTCACGAGT 489 37 199 Antisense
GGCTCACGAGTATCACATTCGATTC 62 539 213 Antisense
CGGCCGGCGCAAGATGTTTTAGACA 637 213 259 Antisense
TTCACGCGCATCCAGGACGTCATAA 200 683 314 Antisense
ACTGATGACTCCATATTGGCTCCGT 564 97 426 Antisense
TGGCTCCGTCCAAAGCATTATCCAA 500 545 442 Antisense
TGATCCATATTCTGCCTATAACGTC 27 567 468 Antisense
CTATAACGTCCGCCATAACGTCACC 231 213 483 Antisense
ACCTCACCACGTGTCGATGATCGTG 461 85 505 Antisense
Sequence Source Affymetrix Proprietary Database