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[177393_at]

Description F58G1.1 /REP_DB=WormBase Gene ID /WP=CE17921 /TR=O62275 /GB=CAB04524.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 41      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 24.9
(-1.5)
A 		45.4
(0.5)
A 		70.6
(3)
A 		10.3
(-3)
A 		60
B 33.9
(-2.8)
A 		50.2
(3)
P 		33.2
(-3)
M 		45.5
(1.3)
A 		17
C 8.5
(-3)
A 		60.5
(3)
M 		35.9
(0.2)
P 		45.6
(1.3)
A 		52
avr 22.4
(-3) 		52
(3) 		46.6
(1.9) 		33.8
(-0.7) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 10.9
(-2.8)
A 		5.7
(-3)
A 		28.3
(-2.3)
A 		70.3
(-1)
P 		133.3
(0.9)
P 		200.6
(3)
P 		195
B 6.9
(-3)
A 		25
(-2.2)
A 		27.4
(-2)
A 		53.1
(-0.8)
P 		90.9
(0.9)
P 		135.8
(3)
P 		129
C 19.9
(-3)
A 		50.7
(-2.1)
A 		42.6
(-2.3)
A 		65.9
(-1.6)
A 		218.5
(3)
P 		127.9
(0.3)
P 		199
avr 12.6
(-3) 		27.1
(-2.4) 		32.8
(-2.1) 		63.1
(-0.9) 		147.6
(2.7) 		154.8
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 9.1
(-0.3)
A 		9.9
(-0)
A 		18.4
(3)
A 		1.5
(-3)
A 		17
B 10.8
(1)
A 		12.4
(3)
P 		7.7
(-3)
M 		9.7
(-0.4)
A 		5
C 2.8
(-3)
A 		17.4
(3)
M 		12.5
(1)
P 		7.7
(-1)
A 		15
avr 7.6
(-1.9) 		13.2
(3) 		12.9
(2.7) 		6.3
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 2
(-3)
A 		2
(-3)
A 		6.2
(-2.5)
A 		17.8
(-1)
P 		40.2
(1.9)
P 		48.8
(3)
P 		47
B 2.4
(-3)
A 		8.3
(-2.3)
A 		6.7
(-2.5)
A 		14.1
(-1.5)
P 		36.9
(1.3)
P 		50.6
(3)
P 		48
C 7.4
(-3)
A 		15.5
(-2.2)
A 		10.8
(-2.7)
A 		13.3
(-2.4)
A 		70.7
(3)
P 		42.8
(0.4)
P 		63
avr 3.9
(-3) 		8.6
(-2.4) 		7.9
(-2.5) 		15.1
(-1.5) 		49.3
(3) 		47.4
(2.7) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177393_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2763
Sequence Type Exemplar sequence
Representative Public ID F58G1.1 NCBI
Target Description F58G1.1 /REP_DB=WormBase Gene ID /WP=CE17921 /TR=O62275 /GB=CAB04524.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064350(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064350 NCBI Caenorhabditis elegans F58G1.1 (F58G1.1) mRNA, complete cds. 11/11 A 
SNAP00000003685 ENSEMBL cdna:SNAP chromosome:CEL140:II:12919474:12922829:1 11/11 None
GENEFINDER00000003700 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12919474:12922829:1 11/11 None
F58G1.1 ENSEMBL cdna:known chromosome:CEL140:II:12919474:12923072:1 gene:F58G1.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011345 1/11 Cross Hyb Matching Probes None
GENEFINDER00000011347 1/11 Cross Hyb Matching Probes None
C06A1.4 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12919470-12922826(+) 100.0 100.0
chrII:10574749-10578101(-) 92.03 92.03
Public Domain and Genome References
Gene Title
Ensembl F58G1.1
Entrez Gene 174932 Entrez gene
SwissProt O62275 EMBL-EBI
WormBase CE17921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04524 Hypothetical protein F58G1.1 [Caenorhabditis elegans] ref|NP_496751.1| F58G1.1 [Caenorhabditis elegans] 0.0
blast T18974 hypothetical protein C06A1.4 - Caenorhabditis elegans 0.0
blast AAF60414 Paz/piwi domain-containing protein 2 [Caenorhabditis elegans] ref|NP_491535.1| PAZ/PIWI domain-containing family member (ppw-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.5E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.5E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:177393_AT
cctacgagattcctcttgctcgtgctgctatgagagatttctcacctgatattcaacttg
tatacattgtggtgtcaaaggatcacagcttcagattcttcaagcccgatttggcaagtc
tagcttcccgtccacaagcgacttccagcaccgcaagcaggcattctgcaatgccagccg
ccccaaaagcatgggatctgaatattgcacccggtattttggtcgattccatcgtgacaa
atccggcttgcaagcagtttttcctgaattcccacatcactctgcaaggaactgcgaaga
ctccattgtacacagttctcgccgatgacgccaaagtcagcatgacagctttggaggata
tcacgtacaagctgtgccatcttcaccagatcgttggacttccgacgtcgcttccaactc
cattgtacgtcgccaacgagtacgccaagagaggacgcaatctgtggaacgaggcagtgg
ccttgaacaacgttccaactgtttctggcccggaagcagatcgtctcaaggagttgacca
agagcatctgctacaaagcgtccgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTACGAGATTCCTCTTGCTCGTGC 488 267 2315 Antisense
TCTTGCTCGTGCTGCTATGAGAGAT 298 617 2328 Antisense
GAGAGATTTCTCACCTGATATTCAA 624 395 2346 Antisense
AGCCCGATTTGGCAAGTCTAGCTTC 235 83 2417 Antisense
TGCACCCGGTATTTTGGTCGATTCC 649 577 2520 Antisense
CCGGCTTGCAAGCAGTTTTTCCTGA 554 263 2557 Antisense
ACTCCATTGTACGTCGCCAACGAGT 163 97 2731 Antisense
GAGGCAGTGGCCTTGAACAACGTTC 47 405 2785 Antisense
GAACAACGTTCCAACTGTTTCTGGC 484 351 2799 Antisense
TTTCTGGCCCGGAAGCAGATCGTCT 495 669 2816 Antisense
AGAGCATCTGCTACAAAGCGTCCGG 22 69 2855 Antisense
Sequence Source Affymetrix Proprietary Database