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[177387_at]

Description M153.2 /REP_DB=WormBase Gene ID /WP=CE03512 /TR=Q21545 /GB=CAA91944.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 57      4grp_total > 10      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 28.6
(-3)
A 		54
(-0.1)
A 		43.7
(-1.3)
M 		81.9
(3)
A 		53
B 76.4
(1.6)
P 		64
(-3)
A 		68.8
(-1.2)
P 		80.2
(3)
P 		16
C 46.9
(-1.8)
A 		48
(-1.6)
A 		39.4
(-3)
A 		76.1
(3)
P 		37
avr 50.6
(-3) 		55.3
(-2) 		50.6
(-3) 		79.4
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 66.6
(2.8)
P 		42.5
(-0.5)
A 		51.8
(0.8)
P 		23.5
(-3)
A 		57.9
(1.6)
A 		68.4
(3)
M 		45
B 48.8
(1.4)
P 		37.3
(-0.6)
A 		22.7
(-3)
A 		41.7
(0.2)
A 		23.2
(-2.9)
A 		58.7
(3)
P 		36
C 25.4
(-3)
A 		44.9
(-1.3)
P 		46.7
(-1.1)
P 		93.9
(3)
A 		33
(-2.3)
M 		49.3
(-0.9)
A 		69
avr 46.9
(-0.4) 		41.6
(-2) 		40.4
(-2.3) 		53
(1.3) 		38
(-3) 		58.8
(3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 10.4
(-3)
A 		11.7
(1.9)
A 		11.4
(0.8)
M 		12
(3)
A 		2
B 24.4
(3)
P 		15.8
(-3)
A 		15.9
(-2.9)
P 		17.1
(-2.1)
P 		9
C 15.5
(3)
A 		13.8
(-0.8)
A 		13.7
(-1)
A 		12.8
(-3)
P 		3
avr 16.8
(3) 		13.8
(-2.8) 		13.7
(-3) 		14
(-2.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 12.3
(0.3)
P 		15.1
(1.7)
A 		11.3
(-0.2)
P 		6
(-3)
A 		17.5
(3)
A 		16.7
(2.6)
M 		12
B 17
(1.2)
P 		12.4
(-0.5)
A 		5.5
(-3)
A 		11
(-1)
A 		9.4
(-1.6)
A 		21.9
(3)
P 		16
C 9.4
(-3)
A 		13.7
(-0.3)
P 		11.9
(-1.4)
P 		18.9
(3)
A 		10.7
(-2.2)
M 		16.5
(1.5)
A 		10
avr 12.9
(-0.7) 		13.7
(-0.2) 		9.6
(-3) 		12
(-1.4) 		12.5
(-1) 		18.4
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177387_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16132
Sequence Type Exemplar sequence
Representative Public ID M153.2 NCBI
Target Description M153.2 /REP_DB=WormBase Gene ID /WP=CE03512 /TR=Q21545 /GB=CAA91944.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077632(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077632 NCBI Caenorhabditis elegans M153.2 (M153.2) mRNA, complete cds. 11/11 None
SNAP00000043669 ENSEMBL cdna:SNAP chromosome:CEL140:X:12151532:12154000:-1 11/11 None
GENEFINDER00000043674 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12151532:12154008:-1 11/11 None
M153.2 ENSEMBL cdna:known chromosome:CEL140:X:12151280:12154000:-1 gene:M153.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12151531-12154000(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M153.2
Entrez Gene 187483 Entrez gene
SwissProt Q21545 EMBL-EBI
WormBase CE36657 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584348_AT similar to CG18549-PA cfa
CANINE_2:CFA.5250.1.A1_AT similar to CG18549-PA cfa
CANINE_2:CFAAFFX.8682.1.S1_S_AT similar to CG18549-PA cfa
DROSOPHILA_2:1625324_AT dm
DROSGENOME1:149779_AT dm
CHICKEN:GGA.8281.1.S1_AT similar to RIKEN cDNA 2600014M03 gga
CHICKEN:GGAAFFX.1165.1.S1_S_AT similar to RIKEN cDNA 2600014M03 gga
CHICKEN:GGAAFFX.25015.1.A1_AT similar to RIKEN cDNA 2600014M03 gga
CHICKEN:GGAAFFX.25015.1.A1_S_AT similar to RIKEN cDNA 2600014M03 gga
HG-U133_PLUS_2:221192_X_AT hypothetical protein ET hs
HG-U133A:221192_X_AT hypothetical protein ET hs
HG-U133A_2:221192_X_AT hypothetical protein ET hs
HU35KSUBA:RC_AA609117_AT hypothetical protein ET hs
HG-U133B:223242_S_AT hypothetical protein ET hs
HG-U133_PLUS_2:223242_S_AT hypothetical protein ET hs
U133_X3P:G2708504_3P_A_AT hypothetical protein ET hs
MG-U74BV2:106520_AT RIKEN cDNA 2600014M03 gene mm
MG-U74BV2:115017_AT RIKEN cDNA 2600014M03 gene mm
MOE430B:1455487_AT RIKEN cDNA 2600014M03 gene mm
MOUSE430_2:1455487_AT RIKEN cDNA 2600014M03 gene mm
MOE430B:1457836_AT RIKEN cDNA 2600014M03 gene mm
MOUSE430_2:1457836_AT RIKEN cDNA 2600014M03 gene mm
RG-U34B:RC_AI058390_AT similar to hypothetical protein ET (predicted) rn
RG-U34B:RC_AA956530_AT similar to hypothetical protein ET (predicted) rn
RAE230B:1391819_AT similar to hypothetical protein ET (predicted) rn
RAT230_2:1391819_AT similar to hypothetical protein ET (predicted) rn
RAE230B:1382196_AT Similar to hypothetical protein ET (predicted) rn
RAT230_2:1382196_AT Similar to hypothetical protein ET (predicted) rn
RAE230A:1375210_AT Similar to hypothetical protein ET (predicted) rn
RAT230_2:1375210_AT Similar to hypothetical protein ET (predicted) rn
RG-U34B:RC_AA955448_AT Similar to hypothetical protein ET (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA91944 Hypothetical protein M153.2 [Caenorhabditis elegans] ref|NP_510033.2| M153.2 [Caenorhabditis elegans] 0.0
blast CAE69879 Hypothetical protein CBG16219 [Caenorhabditis briggsae] 0.0
blast CAE69879 Hypothetical protein CBG16219 [Caenorhabditis briggsae] 1.0E-172
blast CAE69879 Hypothetical protein CBG16219 [Caenorhabditis briggsae] 1.0E-171
blast CAE60772 Hypothetical protein CBG04461 [Caenorhabditis briggsae] 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 1.0E-105
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 1.0E-105
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510033.2 12 9-31,51-72,79-98,103-125,138-160,175-197,226-248,263-285,297-316,331-353,373-392,396-418
M153.2 12 9-31,51-72,79-98,103-125,138-160,175-197,226-248,263-285,297-316,331-353,373-392,396-418
SNAP00000043669 10 9-31,51-72,79-98,103-125,138-160,175-197,226-248,263-285,297-316,331-353
GENEFINDER00000043674 10 12-34,54-75,82-101,106-128,141-163,178-200,229-251,266-288,300-319,334-356
Sequence
Target Sequence 
>C. ELEGANS:177387_AT
tgatccttctcgcatttgtttttgcgtacaccggaattgagcaatctttttggacagcaa
tctacccaacttgcatttcatttaccaaacagctgggtaataacacgaacgctcttttgg
ctcttaacgcaatttgtactgggtttggacaaatcgcagctggagtattctttggtctac
ttggtgacaaatccagaaaaattggccgtgatgccatcattctctgtggtacaattgttc
atcttgtggtttatgccttgtgctatattaatttccctcaagattcatctctaaagaaaa
ctgacgaaatgggaggacttattcagccaaatttggcaatcgctttgattgccggaggcc
ttttgggatttggcgatgcaatttggaacactcaaatttactctttcttgtgtgatacct
tttccaaacaaagtgctcaagcgttttccctcttcaaattgtatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATCCTTCTCGCATTTGTTTTTGC 603 565 686 Antisense
CTACCCAACTTGCATTTCATTTACC 452 225 747 Antisense
TAACACGAACGCTCTTTTGGCTCTT 521 637 786 Antisense
GTGATGCCATCATTCTCTGTGGTAC 708 483 893 Antisense
CATCTTGTGGTTTATGCCTTGTGCT 371 213 925 Antisense
GCCTTGTGCTATATTAATTTCCCTC 343 279 940 Antisense
TAATTTCCCTCAAGATTCATCTCTA 253 635 954 Antisense
ATTGCCGGAGGCCTTTTGGGATTTG 663 5 1033 Antisense
TACTCTTTCTTGTGTGATACCTTTT 528 645 1084 Antisense
ACAAAGTGCTCAAGCGTTTTCCCTC 41 113 1113 Antisense
CGTTTTCCCTCTTCAAATTGTATCA 397 245 1127 Antisense
Sequence Source Affymetrix Proprietary Database