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[177379_at]

Description ZC8.6 /REP_DB=WormBase Gene ID /WP=CE28170 /TR=Q23083 /GB=AAB52625.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 03      4grp_highest_8_8 > 07      4grp_high_8_8 > 29      6grp_total > 05      4grp_total > 26      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 233.6
(-3)
P 		289.8
(3)
P 		253.8
(-0.8)
P 		272.4
(1.1)
P 		56
B 229.4
(-3)
P 		304
(1.5)
P 		268.8
(-0.6)
P 		329.6
(3)
P 		100
C 251.4
(-0.7)
P 		296.9
(1.3)
P 		196.2
(-3)
P 		338.2
(3)
P 		142
avr 238.1
(-3) 		296.9
(1.7) 		239.6
(-2.9) 		313.4
(3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 234.7
(2.3)
P 		184.8
(-0.7)
P 		148
(-3)
A 		245.7
(3)
M 		172.7
(-1.5)
P 		162.9
(-2.1)
P 		98
B 202.4
(1.6)
P 		222.8
(2.8)
P 		225.4
(3)
P 		157.3
(-1)
M 		201.5
(1.6)
A 		123.8
(-3)
A 		102
C 261.9
(3)
P 		182
(-0.9)
P 		210.9
(0.5)
P 		140
(-3)
P 		180.6
(-1)
A 		158
(-2.1)
A 		122
avr 233
(3) 		196.5
(0.4) 		194.8
(0.3) 		181
(-0.7) 		184.9
(-0.4) 		148.2
(-3) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 85.1
(3)
P 		62.9
(0.1)
P 		66.3
(0.5)
P 		39.9
(-3)
P 		45
B 73.1
(2.1)
P 		75.1
(3)
P 		62.3
(-3)
P 		70.2
(0.7)
P 		13
C 83.2
(2.5)
P 		85.4
(3)
P 		68.4
(-0.6)
P 		57
(-3)
P 		28
avr 80.5
(3) 		74.5
(1.5) 		65.7
(-0.6) 		55.7
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 43.3
(-1)
P 		65.7
(3)
P 		32.2
(-3)
A 		62.3
(2.4)
M 		52.1
(0.6)
P 		39.7
(-1.7)
P 		34
B 70.4
(1.3)
P 		74.2
(1.9)
P 		54.7
(-1.1)
P 		41.7
(-3)
M 		81.9
(3)
A 		46.2
(-2.3)
A 		40
C 97.1
(3)
P 		55.5
(-0.6)
P 		53.7
(-0.8)
P 		28.2
(-3)
P 		58.5
(-0.4)
A 		52.9
(-0.8)
A 		69
avr 70.3
(3) 		65.1
(1.8) 		46.9
(-2.4) 		44.1
(-3) 		64.2
(1.6) 		46.3
(-2.5) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177379_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16920
Sequence Type Exemplar sequence
Representative Public ID ZC8.6 NCBI
Target Description ZC8.6 /REP_DB=WormBase Gene ID /WP=CE28170 /TR=Q23083 /GB=AAB52625.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076448(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076448 NCBI Caenorhabditis elegans ZC8.6 (ZC8.6) mRNA, complete cds. 9/11 A 
SNAP00000002219 ENSEMBL cdna:SNAP chromosome:CEL140:X:4993196:4995278:-1 9/11 None
GENEFINDER00000002227 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4993271:4997546:-1 10/11 None
ZC8.6 ENSEMBL cdna:known chromosome:CEL140:X:4972568:4997565:-1 gene:ZC8.6 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028629 1/11 Cross Hyb Matching Probes None
GENEFINDER00000029684 1/11 Cross Hyb Matching Probes None
GENEFINDER00000031499 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4993786-4995108(-) 66.51 66.51
Public Domain and Genome References
Gene Title
Ensembl ZC8.6
Entrez Gene 180770 Entrez gene
SwissProt Q23083 EMBL-EBI
WormBase CE38988 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508849 ZC8.6 [Caenorhabditis elegans] 0.0
blast AAM69079 Hypothetical protein ZC8.6 [Caenorhabditis elegans] 1.0E-140
blast CAE68673 Hypothetical protein CBG14577 [Caenorhabditis briggsae] 1.0E-121
blast NP_508849 ZC8.6 [Caenorhabditis elegans] 1.0E-104
blast AAM69079 Hypothetical protein ZC8.6 [Caenorhabditis elegans] 1.0E-103
blast NP_508849 ZC8.6 [Caenorhabditis elegans] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 3.0E-18
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 5.4E-29
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 3.0E-18
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 3.0E-18
Sequence
Target Sequence 
>C. ELEGANS:177379_AT
cgctccggaggatcctcgaggacaatttattttattttttttttccaaaaaaatgtgtct
ttttaattattttttttcaaaaatgaacaattttaatgttaaatttatttcttggcagtc
gtcggccatttctccaagtcgccgtaacattcgaatgtcgtcgtctggagcgtctgaatc
agaagtagaaacttgcgatgagaacgttggattgttgggatcccgtgggaagaaagtgaa
gcatgagagatcgaacagagctgagcaaactgctccacttcatagacctcttggaagcca
cgccgatgaagcaacaattgaatttggtggcagaagcgactatcacgatccatacacttc
tgatgacgaattgttgcacacaggaagcgatgcatatggatcaacagtcggagcaacgat
ctctttgaagttcaacgaagcgtgtcgtgccattcacaatggtcactacccacagagaat
tgctcagggttccagtggtagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCTCCGGAGGATCCTCGAGGACAA 433 255 117 Antisense
ATTTCTTGGCAGTCGTCGGCCATTT 342 15 223 Antisense
ATTTCTCCAAGTCGCCGTAACATTC 279 17 244 Antisense
TTCGAATGTCGTCGTCTGGAGCGTC 20 693 266 Antisense
GAGCAAACTGCTCCACTTCATAGAC 212 391 379 Antisense
TTCATAGACCTCTTGGAAGCCACGC 511 685 395 Antisense
GAAGCCACGCCGATGAAGCAACAAT 625 343 410 Antisense
GAAGCGACTATCACGATCCATACAC 686 343 449 Antisense
AGCGTGTCGTGCCATTCACAATGGT 640 81 555 Antisense
GGTCACTACCCACAGAGAATTGCTC 619 501 577 Antisense
AATTGCTCAGGGTTCCAGTGGTAGT 440 177 594 Antisense
Sequence Source Affymetrix Proprietary Database