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[177361_s_at]

Description ZK20.4 /REP_DB=WormBase Gene ID /WP=CE06607 /TR=Q23450 /GB=CAA93779.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 91      6grp_total > 67      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 430
1 2 3 4 range
A 186.6
(-3)
P
219.4
(-2.4)
P
192.3
(-2.9)
P
492.8
(3)
P
306
B 156.9
(-2)
P
93.8
(-3)
P
131
(-2.4)
P
460.8
(3)
P
367
C 185
(-2.9)
P
182.9
(-2.9)
P
179.4
(-3)
P
523.5
(3)
P
344
avr 176.2
(-2.8)
165.4
(-3)
167.6
(-3)
492.4
(3)
327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 199.4
(-1.3)
P
216
(-0.3)
P
270.2
(3)
P
223.7
(0.1)
P
172.5
(-3)
P
181.4
(-2.5)
P
98
B 175
(-2.6)
P
241.6
(-0.2)
P
216.3
(-1.1)
P
162.2
(-3)
P
203.2
(-1.6)
P
334.6
(3)
P
172
C 190.2
(-1.2)
P
204.5
(-0.8)
P
120.5
(-3)
P
220.6
(-0.4)
P
182.2
(-1.4)
P
347.6
(3)
P
227
avr 188.2
(-2.9)
220.7
(-1)
202.3
(-2)
202.2
(-2)
186
(-3)
287.9
(3)
102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 67.9
(2)
P
47.6
(-3)
P
50.3
(-2.3)
P
72.1
(3)
P
25
B 50
(-0.9)
P
23.2
(-3)
P
30.3
(-2.4)
P
98.2
(3)
P
75
C 61.3
(-1.5)
P
52.6
(-3)
P
62.5
(-1.3)
P
88.3
(3)
P
36
avr 59.7
(-0.5)
41.1
(-3)
47.7
(-2.1)
86.2
(3)
45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 36.8
(-3)
P
76.8
(3)
P
58.8
(0.3)
P
56.7
(-0)
P
52.1
(-0.7)
P
44.2
(-1.9)
P
40
B 60.9
(-1.7)
P
80.4
(-0.3)
P
52.5
(-2.3)
P
43
(-3)
P
82.5
(-0.1)
P
124.8
(3)
P
82
C 70.5
(-0.2)
P
62.4
(-0.8)
P
30.7
(-3)
P
44.4
(-2)
P
59
(-1)
P
116.4
(3)
P
86
avr 56.1
(-1.9)
73.2
(0.3)
47.3
(-3)
48
(-2.9)
64.5
(-0.8)
95.1
(3)
48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177361_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2855
Sequence Type Exemplar sequence
Representative Public ID ZK20.4 NCBI
Target Description ZK20.4 /REP_DB=WormBase Gene ID /WP=CE06607 /TR=Q23450 /GB=CAA93779.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064086(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_064086 NCBI Caenorhabditis elegans ZK20.4 (ZK20.4) mRNA, complete cds. 11/11 None
SNAP00000019229 ENSEMBL cdna:SNAP chromosome:CEL140:II:11649104:11651053:-1 11/11 None
GENEFINDER00000019236 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11649104:11652633:-1 11/11 None
ZK20.4 ENSEMBL cdna:known chromosome:CEL140:II:11649066:11651053:-1 gene:ZK20.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11649100-11651050(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK20.4
Entrez Gene 174784 Entrez gene
SwissProt Q23450 EMBL-EBI
WormBase CE06607 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6536.1.S1_AT similar to CG13865-PA.3 cfa
CHICKEN:GGA.7949.2.S1_A_AT similar to RIKEN cDNA 2010305A19 gga
HG-U133A:219420_S_AT chromosome 1 open reading frame 163 hs
HG-U133_PLUS_2:219420_S_AT chromosome 1 open reading frame 163 hs
HG-U133A_2:219420_S_AT chromosome 1 open reading frame 163 hs
U133_X3P:G12751490_3P_A_AT chromosome 1 open reading frame 163 hs
U133_X3P:HS.164371.0.S2_3P_AT chromosome 1 open reading frame 163 hs
HG-U133_PLUS_2:222883_AT chromosome 1 open reading frame 163 hs
HG-U133B:222883_AT chromosome 1 open reading frame 163 hs
HG-U95E:71112_AT chromosome 1 open reading frame 163 hs
HG-U95D:84703_AT chromosome 1 open reading frame 163 hs
MG-U74BV2:108499_AT RIKEN cDNA 2010305A19 gene mm
MG-U74BV2:164086_AT RIKEN cDNA 2010305A19 gene mm
MOE430A:1424520_AT RIKEN cDNA 2010305A19 gene mm
MOUSE430A_2:1424520_AT RIKEN cDNA 2010305A19 gene mm
MOUSE430_2:1424520_AT RIKEN cDNA 2010305A19 gene mm
MOE430B:1440769_AT RIKEN cDNA 2010305A19 gene, mRNA (cDNA clone MGC:8003 IMAGE:3585966) mm
MOUSE430_2:1440769_AT RIKEN cDNA 2010305A19 gene, mRNA (cDNA clone MGC:8003 IMAGE:3585966) mm
MU19KSUBB:TC27531_AT RIKEN cDNA 2010305A19 gene, mRNA (cDNA clone MGC:8003 IMAGE:3585966) mm
MU19KSUBB:TC27531_G_AT RIKEN cDNA 2010305A19 gene, mRNA (cDNA clone MGC:8003 IMAGE:3585966) mm
RG-U34B:RC_AI058886_AT similar to RIKEN cDNA 2010305A19 (predicted) rn
RAE230A:1372611_AT similar to RIKEN cDNA 2010305A19 (predicted) rn
RAT230_2:1372611_AT similar to RIKEN cDNA 2010305A19 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA93779 Hypothetical protein ZK20.4 [Caenorhabditis elegans] ref|NP_496487.1| ZK20.4 [Caenorhabditis elegans] 1.0E-136
blast CAE59460 Hypothetical protein CBG02840 [Caenorhabditis briggsae] 1.0E-126
blast CAA93780 Hypothetical protein ZK20.3 [Caenorhabditis elegans] ref|NP_496488.1| ZK20.3 [Caenorhabditis elegans] 1.0E-123
blast EAA00460 ENSANGP00000015777 [Anopheles gambiae str. PEST] ref|XP_320499.2| ENSANGP00000015777 [Anopheles gambiae str. PEST] 4.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 4.3E-20
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 6.8E-11
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 3.7E-6
Sequence
Target Sequence
>C. ELEGANS:177361_S_AT
ggcgaatacatggaagctctgcaacaaaattttcaagcggccgtagcgattttcaagaag
aactgtgacgagaacagttatccaaagtcatgtttcaagtacggaatgtacatgttagcc
ggaaaagttggagacgatgcaagtctgtcaaaaatgattcgaccgatgaaaatcgcatgt
gatgcgaatattccacaaggatgtcgatatttatcacttgttcattggaatggagaaaaa
gatcggaaagctgattcagaaaaggctgaacgatacatgagaagagcctgtgaactggaa
gatggtgaagcttgctggttgttgagtacctggtatatgggaaataaggagaaatttaag
acgaatgctgcaggagaggctaaggagctggaccgtgccgaacttggcagtctgtcacgt
gatatggacaaaagcctccagtttgcgattcgagc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GGCGAATACATGGAAGCTCTGCAAC 231 543 142 Antisense
GCAACAAAATTTTCAAGCGGCCGTA 193 315 162 Antisense
CAAGCGGCCGTAGCGATTTTCAAGA 498 185 175 Antisense
CAGTTATCCAAAGTCATGTTTCAAG 172 207 216 Antisense
GGATGTCGATATTTATCACTTGTTC 275 515 340 Antisense
GAAGAGCCTGTGAACTGGAAGATGG 293 339 422 Antisense
GTGAAGCTTGCTGGTTGTTGAGTAC 516 481 446 Antisense
GAGGCTAAGGAGCTGGACCGTGCCG 143 401 517 Antisense
GACCGTGCCGAACTTGGCAGTCTGT 256 383 532 Antisense
CTTGGCAGTCTGTCACGTGATATGG 561 217 544 Antisense
AAAGCCTCCAGTTTGCGATTCGAGC 131 125 572 Antisense
Sequence Source Affymetrix Proprietary Database