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[177357_at]

Description M176.3 /REP_DB=WormBase Gene ID /WP=CE12464 /TR=Q21551 /GB=CAB01650.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 12      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 23      6grp_total > 23      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1891
1 2 3 4 range
A 2247.6
(1.5)
P 		2709.9
(3)
P 		2645.7
(2.8)
P 		869.4
(-3)
P 		1841
B 2355.5
(2.2)
P 		2509.1
(2.8)
P 		2563.9
(3)
P 		911.1
(-3)
P 		1653
C 2702.6
(3)
P 		2405.2
(2.1)
P 		2582.7
(2.6)
P 		819.3
(-3)
P 		1883
avr 2435.2
(2.4) 		2541.4
(2.8) 		2597.4
(3) 		866.6
(-3) 		1731

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1306
1 2 3 4 5 6 range
A 2411.2
(2.6)
P 		2473.6
(3)
P 		2359.8
(2.3)
P 		1850.6
(-0.6)
P 		1664.7
(-1.6)
P 		1425.6
(-3)
P 		1048
B 2572.9
(3)
P 		2323.2
(1.7)
P 		2560.7
(2.9)
P 		1870.4
(-0.5)
P 		1788.3
(-0.9)
P 		1376
(-3)
P 		1197
C 2681.9
(3)
P 		2337.4
(1.2)
P 		2270.8
(0.8)
P 		1845.3
(-1.4)
P 		1751
(-1.9)
P 		1540.9
(-3)
P 		1141
avr 2555.3
(3) 		2378.1
(2) 		2397.1
(2.1) 		1855.4
(-0.8) 		1734.7
(-1.4) 		1447.5
(-3) 		1108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 773
1 2 3 4 range
A 818.4
(3)
P 		588.3
(1)
P 		691.6
(1.9)
P 		127.3
(-3)
P 		691
B 751.2
(3)
P 		619.7
(1.6)
P 		594.1
(1.3)
P 		194.2
(-3)
P 		557
C 894.6
(3)
P 		691.5
(1.4)
P 		900.2
(3)
P 		138.1
(-3)
P 		762
avr 821.4
(3) 		633.2
(1.3) 		728.6
(2.2) 		153.2
(-3) 		668

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 647
1 2 3 4 5 6 range
A 444.5
(-1.9)
P 		879.7
(3)
P 		513.9
(-1.1)
P 		469.4
(-1.6)
P 		502.3
(-1.3)
P 		347.1
(-3)
P 		533
B 895.2
(3)
P 		773.3
(1.2)
P 		621.8
(-1.1)
P 		495.4
(-3)
P 		726.6
(0.5)
P 		513.1
(-2.7)
P 		400
C 994.1
(3)
P 		713.2
(0.3)
P 		578
(-1)
P 		371.1
(-3)
P 		566.6
(-1.1)
P 		515.8
(-1.6)
P 		623
avr 777.9
(2.8) 		788.7
(3) 		571.2
(-0.8) 		445.3
(-3) 		598.5
(-0.3) 		458.7
(-2.8) 		343
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177357_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2615
Sequence Type Exemplar sequence
Representative Public ID M176.3 NCBI
Target Description M176.3 /REP_DB=WormBase Gene ID /WP=CE12464 /TR=Q21551 /GB=CAB01650.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063611(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063611 NCBI Caenorhabditis elegans M176.3 (M176.3) mRNA, complete cds. 11/11 None
SNAP00000025001 ENSEMBL cdna:SNAP chromosome:CEL140:II:9416925:9418028:1 11/11 None
GENEFINDER00000025013 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9416925:9418028:1 11/11 None
M176.3.2 ENSEMBL cdna:known chromosome:CEL140:II:9416921:9418231:1 gene:M176.3 11/11 None
M176.3.1 ENSEMBL cdna:known chromosome:CEL140:II:9416925:9418231:1 gene:M176.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9416921-9418025(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M176.3
Entrez Gene 174494 Entrez gene
SwissProt Q21551 EMBL-EBI
WormBase CE12464 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01650 Hypothetical protein M176.3 [Caenorhabditis elegans] ref|NP_496012.1| M176.3 [Caenorhabditis elegans] 6.0E-79
blast CAE67814 Hypothetical protein CBG13394 [Caenorhabditis briggsae] 3.0E-70
Sequence
Target Sequence 
>C. ELEGANS:177357_AT
gggagcatcccagagtgcagaacaagaggcacgtcccgaagtggtgcgcattgaccgaaa
tgagattcccgaggagtacaaaaccgtcggtgtatcatcggacgttgtgagccgagtcaa
tgcgaccagagttgccggaaacgacggagaaagtgaccgtctcagacaagaattggctcg
agagcgtgaagaaaaagctagattaagagaagatatggctaagctcagtcagctgcaaca
gagaaagaccgctggaatttccgccgccccagtttcgatttccggaaatgatttggagga
gagaaagaaaattttcgatgacactgttgaacgcgttcagaagcaattcttcgcttatca
ccgtgaaaatgtgtgccaggataatgaaaatgagattgtaagatgtcttcaagaaaatcc
aggacgtgttttgaagtgtgctccacttactgaggcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGAGCATCCCAGAGTGCAGAACAA 587 493 15 Antisense
CAGAACAAGAGGCACGTCCCGAAGT 378 141 32 Antisense
GTCCCGAAGTGGTGCGCATTGACCG 499 473 47 Antisense
AACCGTCGGTGTATCATCGGACGTT 494 147 96 Antisense
CGGACGTTGTGAGCCGAGTCAATGC 310 243 113 Antisense
GAGTCAATGCGACCAGAGTTGCCGG 305 395 128 Antisense
GGCTAAGCTCAGTCAGCTGCAACAG 219 527 231 Antisense
GAGAAAGACCGCTGGAATTTCCGCC 222 391 255 Antisense
GCCCCAGTTTCGATTTCCGGAAATG 424 283 280 Antisense
GAAGCAATTCTTCGCTTATCACCGT 661 339 354 Antisense
AGTGTGCTCCACTTACTGAGGCATT 391 59 449 Antisense
Sequence Source Affymetrix Proprietary Database