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[177353_s_at]

Description M199.4 /REP_DB=WormBase Gene ID /WP=CE20021 /TR=Q9NAP0 /GB=CAB70254.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 25      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 15.1
(-3)
A 		27.7
(-1.1)
A 		41.2
(0.9)
A 		55.6
(3)
A 		41
B 19.9
(-3)
A 		36.3
(3)
P 		23.5
(-1.7)
A 		28
(-0)
A 		16
C 21.6
(-2.3)
A 		38.6
(-0.3)
A 		16.1
(-3)
A 		65.4
(3)
P 		49
avr 18.9
(-3) 		34.2
(-0) 		26.9
(-1.4) 		49.7
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 77.5
(3)
A 		1.3
(-3)
A 		12.6
(-2.1)
A 		9.6
(-2.3)
A 		39.8
(0)
P 		2.3
(-2.9)
A 		76
B 16.3
(0.5)
A 		20.5
(1.6)
A 		3.4
(-3)
A 		25.6
(3)
A 		12.5
(-0.5)
A 		22.4
(2.1)
A 		22
C 36.2
(3)
P 		13.5
(-1.7)
A 		20.8
(-0.2)
A 		7.2
(-3)
A 		28
(1.3)
M 		10.9
(-2.2)
A 		29
avr 43.3
(3) 		11.8
(-3) 		12.3
(-2.9) 		14.1
(-2.6) 		26.8
(-0.1) 		11.9
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 5.5
(-3)
A 		6
(-2.4)
A 		10.8
(3)
A 		8.1
(-0.1)
A 		5
B 6.4
(-1.3)
A 		9
(3)
P 		5.4
(-3)
A 		6
(-2)
A 		4
C 7.2
(-1.3)
A 		11.1
(3)
A 		5.6
(-3)
A 		11
(2.9)
P 		6
avr 6.4
(-3) 		8.7
(3) 		7.3
(-0.7) 		8.4
(2.2) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 14.3
(3)
A 		0.5
(-3)
A 		2.7
(-2)
A 		2.4
(-2.2)
A 		12
(2)
P 		0.6
(-3)
A 		14
B 5.7
(0.9)
A 		6.8
(1.8)
A 		0.8
(-3)
A 		6.8
(1.8)
A 		5.1
(0.4)
A 		8.3
(3)
A 		8
C 13.4
(3)
P 		4.1
(-1.7)
A 		5.3
(-1.1)
A 		1.5
(-3)
A 		9.1
(0.8)
M 		3.6
(-1.9)
A 		12
avr 11.1
(3) 		3.8
(-2.3) 		2.9
(-3) 		3.6
(-2.5) 		8.7
(1.2) 		4.2
(-2) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177353_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9111
Sequence Type Exemplar sequence
Representative Public ID M199.4 NCBI
Target Description M199.4 /REP_DB=WormBase Gene ID /WP=CE20021 /TR=Q9NAP0 /GB=CAB70254.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070425(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070425 NCBI Caenorhabditis elegans M199.4 (M199.4) mRNA, complete cds. 11/11 None
GENEFINDER00000027576 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15124942:15127743:-1 11/11 None
M199.4 ENSEMBL cdna:known chromosome:CEL140:IV:15124942:15126070:-1 gene:M199.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027571 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15124947-15126076(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M199.4
Entrez Gene 178423 Entrez gene
SwissProt Q9NAP0 EMBL-EBI
WormBase CE20021 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70254 Hypothetical protein M199.4 [Caenorhabditis elegans] ref|NP_502826.1| M199.4 [Caenorhabditis elegans] 1.0E-115
Sequence
Target Sequence 
>C. ELEGANS:177353_S_AT
tgaacgcgggccgcgagggcgtgaaggacgtgaaggaccacgtggagctcaaggtgcccc
agggccaaacggaaccggatttttctggccaggaacatgcgagcaatccgattgtccaga
cggttggatctcggttaatcgaaaaatcaataatgatgtagaaaagaaatggtgcatcaa
catgttctggaaccaagcaaccaacgatttatataaaacctcggaaaaagtgtgcaacag
ctatggtggtgtgatgactggattcaaggacaagggggaattcatttctgtttttgctct
actcaaaaatcgattcccgaacattaaacctgtatcacaagttatgattggggcacgcag
attggagacatgtgcttcgatatcgaattttggcaattgtacgataacaaagggaacaaa
atggactgatcttgtaatgtcgattgattcctttttcaatgaattgcagcaatctgcccg
tttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACGCGGGCCGCGAGGGCGTGAA 101 573 99 Antisense
TCAAGGTGCCCCAGGGCCAAACGGA 492 629 147 Antisense
GAGCAATCCGATTGTCCAGACGGTT 678 389 199 Antisense
CAGACGGTTGGATCTCGGTTAATCG 482 197 215 Antisense
GGAACCAAGCAACCAACGATTTATA 683 529 287 Antisense
ATTCATTTCTGTTTTTGCTCTACTC 697 11 378 Antisense
GATTCCCGAACATTAAACCTGTATC 708 429 410 Antisense
ATTGGGGCACGCAGATTGGAGACAT 141 3 445 Antisense
TGGAGACATGTGCTTCGATATCGAA 59 549 461 Antisense
GTAATGTCGATTGATTCCTTTTTCA 272 463 532 Antisense
GAATTGCAGCAATCTGCCCGTTTCT 234 323 559 Antisense
Sequence Source Affymetrix Proprietary Database