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[177350_at]

Description M176.4 /REP_DB=WormBase Gene ID /WP=CE12466 /TR=Q21552 /GB=CAB01651.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 62      6grp_total > 60      4grp_total > 51      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 432.7
(3)
P 		331.7
(-3)
P 		382
(-0)
P 		353.1
(-1.7)
P 		101
B 409.6
(0.7)
P 		459.4
(3)
P 		459.9
(3)
P 		331.1
(-3)
P 		129
C 351.4
(-3)
P 		467
(3)
P 		370.6
(-2)
P 		426.7
(0.9)
P 		116
avr 397.9
(0.4) 		419.4
(3) 		404.2
(1.1) 		370.3
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 446.5
(-1.4)
P 		415
(-2.1)
P 		540.7
(0.7)
P 		643.8
(3)
P 		471.2
(-0.8)
P 		373.9
(-3)
P 		270
B 523.5
(0.3)
P 		478
(-1.3)
P 		550
(1.1)
P 		606
(3)
P 		461.9
(-1.8)
P 		425.5
(-3)
P 		181
C 469.7
(-1.2)
P 		454.4
(-1.6)
P 		651.8
(3)
P 		603.4
(1.9)
P 		391.8
(-3)
P 		392.2
(-3)
P 		260
avr 479.9
(-0.7) 		449.1
(-1.6) 		580.8
(2) 		617.7
(3) 		441.6
(-1.8) 		397.2
(-3) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 157.5
(3)
P 		72
(-1.8)
P 		99.9
(-0.3)
P 		51.7
(-3)
P 		106
B 130.6
(3)
P 		113.5
(1.3)
P 		106.6
(0.6)
P 		70.6
(-3)
P 		60
C 116.3
(1.3)
P 		134.3
(3)
P 		129.2
(2.5)
P 		71.9
(-3)
P 		62
avr 134.8
(3) 		106.6
(0.6) 		111.9
(1) 		64.7
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 82.3
(-3)
P 		147.6
(1.8)
P 		117.8
(-0.4)
P 		163.3
(3)
P 		142.2
(1.4)
P 		91
(-2.4)
P 		81
B 182.2
(2.4)
P 		159.1
(-0.2)
P 		133.6
(-3)
P 		160.5
(-0)
P 		187.7
(3)
P 		158.7
(-0.2)
P 		54
C 174.1
(3)
P 		138.6
(-1)
P 		165.9
(2.1)
P 		121.3
(-3)
P 		126.8
(-2.4)
P 		131.3
(-1.9)
P 		53
avr 146.2
(1.6) 		148.4
(2.1) 		139.1
(-0.1) 		148.4
(2.1) 		152.2
(3) 		127
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177350_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2751
Sequence Type Exemplar sequence
Representative Public ID M176.4 NCBI
Target Description M176.4 /REP_DB=WormBase Gene ID /WP=CE12466 /TR=Q21552 /GB=CAB01651.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182267(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182267 NCBI Caenorhabditis elegans M176.11 (M176.11) mRNA, complete cds. 11/11 None
SNAP00000025003 ENSEMBL cdna:SNAP chromosome:CEL140:II:9422182:9423003:1 11/11 None
GENEFINDER00000025012 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9420391:9423003:1 11/11 None
M176.11 ENSEMBL cdna:known chromosome:CEL140:II:9422182:9423003:1 gene:M176.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9420387-9423000(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M176.11
Entrez Gene 353402 Entrez gene
SwissProt Q86NF6 EMBL-EBI
WormBase CE33288 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE67813 Hypothetical protein CBG13393 [Caenorhabditis briggsae] 0.0
blast CAB01651 Hypothetical protein M176.4 [Caenorhabditis elegans] ref|NP_496013.2| M176.4 [Caenorhabditis elegans] 1.0E-129
blast CAD62041 Hypothetical protein M176.11 [Caenorhabditis elegans] ref|NP_872067.1| M176.11 [Caenorhabditis elegans] 1.0E-118
blast CAD62041 Hypothetical protein M176.11 [Caenorhabditis elegans] ref|NP_872067.1| M176.11 [Caenorhabditis elegans] 1.0E-117
blast CAE67813 Hypothetical protein CBG13393 [Caenorhabditis briggsae] 1.0E-111
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_872067.1 6 30-49,69-91,98-120,125-147,167-189,199-216
M176.11 6 30-49,69-91,98-120,125-147,167-189,199-216
SNAP00000025003 6 30-49,69-91,98-120,125-147,167-189,199-216
GENEFINDER00000025012 14 5-24,34-56,63-80,84-103,115-134,144-166,173-195,226-248,260-277,297-319,326-348,353-375,395-417,427-444
Sequence
Target Sequence 
>C. ELEGANS:177350_AT
tcttctcagtgcacctctagtgatcttgtctattttgacacttgcaacaacaatgaatcc
aaaagcccgattcgccactgccatgtcatttttaatgtccgccatcgcgacatacttcca
atcagtcaaccgtactggcccagcttccgctatcttttacacaattgccaatgtgttcta
ttacttctcatatagagacattgtaacttcagtttcttctccaattgtgttcctagccgc
gtgcgtttcattcggtcaattcctccaccttcttcaagatcttctcgttgccatcccatt
cctggctacaatcctcactatccttctagcttctcacgttgtgatcctagccacttctgc
aagtctctgccaaaatggccagcatggagattacgatgctcgtcaagcatctactgttcg
cttgattggtgcagttttggcatggctcagcgctttccttctgctgatcaactcgttcca
aactcataccaaagaacttcactctgtctctcgtattatcttctacctcggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCTCAGTGCACCTCTAGTGATC 197 617 789 Antisense
TCGCCACTGCCATGTCATTTTTAAT 295 599 860 Antisense
TTCCGCTATCTTTTACACAATTGCC 320 699 933 Antisense
TAACTTCAGTTTCTTCTCCAATTGT 166 639 992 Antisense
TTCTTCAAGATCTTCTCGTTGCCAT 197 691 1058 Antisense
AGCTTCTCACGTTGTGATCCTAGCC 252 79 1116 Antisense
CACTTCTGCAAGTCTCTGCCAAAAT 458 197 1140 Antisense
GCTCGTCAAGCATCTACTGTTCGCT 593 299 1186 Antisense
TTCTGCTGATCAACTCGTTCCAAAC 52 695 1247 Antisense
ACCAAAGAACTTCACTCTGTCTCTC 578 83 1276 Antisense
TCTCGTATTATCTTCTACCTCGGAA 407 611 1297 Antisense
Sequence Source Affymetrix Proprietary Database