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[177349_at]

Description M199.3 /REP_DB=WormBase Gene ID /WP=CE20020 /TR=Q9NAP1 /GB=CAB70253.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 72      4grp_total > 21      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 38.9
(1.5)
A 		5.5
(-2.8)
A 		3.8
(-3)
A 		50.4
(3)
A 		47
B 42.1
(2.7)
A 		43.4
(3)
A 		17.9
(-3)
A 		36.8
(1.4)
A 		26
C 16.5
(-3)
A 		32.3
(1.2)
A 		27.3
(-0.2)
A 		39.3
(3)
A 		23
avr 32.5
(0.8) 		27.1
(-0.5) 		16.3
(-3) 		42.2
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 7.5
(-2.1)
A 		1.8
(-3)
A 		37.2
(2.4)
A 		22.1
(0.1)
A 		40.8
(3)
A 		7.8
(-2.1)
A 		39
B 25.3
(0.8)
A 		6.5
(-2.5)
A 		38
(3)
A 		35.7
(2.6)
A 		3.8
(-3)
A 		28.1
(1.3)
A 		34
C 3.1
(-3)
A 		23.3
(1.3)
A 		3.9
(-2.8)
A 		31.5
(3)
A 		18
(0.1)
A 		15.2
(-0.4)
A 		28
avr 12
(-2.5) 		10.5
(-3) 		26.4
(1.9) 		29.8
(3) 		20.9
(0.2) 		17
(-1) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 14.1
(3)
A 		1.2
(-2.9)
A 		1
(-3)
A 		7.4
(-0.1)
A 		13
B 13.4
(3)
A 		10.7
(1.2)
A 		4.2
(-3)
A 		7.8
(-0.7)
A 		9
C 5.5
(-3)
A 		9.3
(2.7)
A 		9.5
(3)
A 		6.6
(-1.4)
A 		4
avr 11
(3) 		7.1
(-0.8) 		4.9
(-3) 		7.3
(-0.6) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 1.4
(-2.6)
A 		0.6
(-3)
A 		8.1
(0.8)
A 		5.6
(-0.4)
A 		12.3
(3)
A 		1.9
(-2.3)
A 		12
B 8.8
(1.9)
A 		2.2
(-2.5)
A 		9.2
(2.1)
A 		9.5
(2.3)
A 		1.5
(-3)
A 		10.5
(3)
A 		9
C 1.2
(-2.8)
A 		7.1
(3)
A 		1
(-3)
A 		6.3
(2.2)
A 		5.8
(1.7)
A 		5.1
(1)
A 		6
avr 3.8
(-2.2) 		3.3
(-3) 		6.1
(1.4) 		7.1
(3) 		6.5
(2.1) 		5.8
(0.9) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177349_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8515
Sequence Type Exemplar sequence
Representative Public ID M199.3 NCBI
Target Description M199.3 /REP_DB=WormBase Gene ID /WP=CE20020 /TR=Q9NAP1 /GB=CAB70253.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070424(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070424 NCBI Caenorhabditis elegans M199.3 (M199.3) mRNA, complete cds. 11/11 None
SNAP00000027567 ENSEMBL cdna:SNAP chromosome:CEL140:IV:15117534:15117785:1 11/11 None
GENEFINDER00000027577 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:15114586:15122510:1 11/11 None
M199.3 ENSEMBL cdna:known chromosome:CEL140:IV:15117534:15117785:1 gene:M199.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:15117539-15117791(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M199.3
Entrez Gene 187501 Entrez gene
SwissProt Q9NAP1 EMBL-EBI
WormBase CE20020 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE17891 Hypothetical protein M199.6 [Caenorhabditis elegans] ref|NP_001023323.1| M199.6 [Caenorhabditis elegans] 1.0E-174
blast CAE17892 Hypothetical protein M199.7 [Caenorhabditis elegans] ref|NP_001023324.1| M199.7 [Caenorhabditis elegans] 2.0E-66
blast CAB70253 Hypothetical protein M199.3 [Caenorhabditis elegans] ref|NP_502825.1| M199.3 [Caenorhabditis elegans] 3.0E-33
blast AAF36070 Hypothetical protein Y77E11A.9 [Caenorhabditis elegans] ref|NP_500080.2| Y77E11A.9 [Caenorhabditis elegans] 5.0E-32
blast CAE68116 Hypothetical protein CBG13759 [Caenorhabditis briggsae] 4.0E-14
blast CAE74034 Hypothetical protein CBG21684 [Caenorhabditis briggsae] 1.0E-13
Sequence
Target Sequence 
>C. ELEGANS:177349_AT
caaaaacagttgctccttacacttggaagactgatgcaggagcccgtgcaacctcaacat
ccgactgcattgttttggtactcaacggaaataacccaattgtggcagatgttcagagct
gcaacgcggatacaactcttcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAAACAGTTGCTCCTTACACTTG 361 189 32 Antisense
CAGTTGCTCCTTACACTTGGAAGAC 605 205 38 Antisense
GCTCCTTACACTTGGAAGACTGATG 211 299 43 Antisense
AAGACTGATGCAGGAGCCCGTGCAA 662 153 58 Antisense
AACCTCAACATCCGACTGCATTGTT 194 147 81 Antisense
ATCCGACTGCATTGTTTTGGTACTC 428 37 90 Antisense
ACTGCATTGTTTTGGTACTCAACGG 300 97 95 Antisense
GTACTCAACGGAAATAACCCAATTG 266 457 109 Antisense
ACCCAATTGTGGCAGATGTTCAGAG 32 91 125 Antisense
GATGTTCAGAGCTGCAACGCGGATA 690 407 139 Antisense
TGCAACGCGGATACAACTCTTCCTG 250 577 151 Antisense
Sequence Source Affymetrix Proprietary Database